|
|
Sanatorium with Speleotherapy for Childern Ostrov u Macochy
Tran, Hoang Long ; Kotek, arch Jakub (referee) ; Odvárka, Antonín (advisor)
The master thesis Sanatorium with Speleotherapy for Children - Ostrov u Macochy is an architectonical concept which consist of the establishment of a sanatorium with various functions for patients and the public. The compound offers a medical block, administrative and economic blocks, a school, a cafeteria for visitors, a guests house for adult clients and complement. The building site is located on an inclined terrain near Císařské jeskyně (Caesar cave) and it is surrounded by forest from three sides. There is a scenic view to the south from the construction site. The neighboring buildings consist only of family houses. The compound of sanatorium aimed to have the closest interaction with surroundings and the terrain, therefore the estate is divided into three different segments based on the three most important functions of the construction program.
|
|
|
Early phase of anti-Leishmania immunity in the host skin
Máčalíková, Bára ; Leštinová, Tereza (advisor) ; Kolářová, Iva (referee)
Leishmania parasites are parasitic protozoans that cause disease called leishmaniasis, which primarily affects mammals. Throughout evolution, Leishmania has adapted to the host's immune system, using it to its advantage. This bachelor's thesis describes the relationship between Leishmania and early immune components in the host's skin, as well as the parasite's ability to inhibit the microbicidal activities of cells. The infection begins with the inoculation of infectious promastigotes into the skin, and before reaching their target cells, Leishmania primarily interacts primarily with the complement system, keratinocytes, fibroblats, eosinophils, neutrophils, mast cells and dendritic cells. Understanding the mutual interaction between the host and the parasite is essential for vaccine development and the treatment of leishmaniasis. KEYWORDS: leishmania, skin, early imunity, complement system, keratinocytes, fibroblasts, eosinophils, neutrophils, mast cells, dendritic cells
|
|
|
Interaction of the human pathogen Bordetella pertussis with blood serum
Štipl, Daniel ; Večerek, Branislav (advisor) ; Kamanová, Jana (referee)
Bordetella pertussis is a Gram-negative strictly human pathogen and the major causative agent of whooping cough or pertussis. The incidence of this highly contagious respiratory disease in developed countries has increased in the last decades. One of the less characterized virulence factors of B. pertussis is the type three secretion system (TTSS) which is responsible for the secretion of the effector proteins into host eukaryotic cells. This diploma thesis sheds light onto factors influencing TTSS in vitro activity. Although TTSS of laboratory strain Tohama I was induced by biologically active compounds present in blood (e. g. complement proteins), TTSS of recent clinical isolate B1917 seems to be induced permanently. Furthermore, BB0302 encoding a GntR family transcription regulator in B. bronchiseptica RB50 (homologous to BP0209 of Tohama I) was studied, however, the deletion of this gene did not affect the TTSS functionality. Serum resistance is a factor that plays a key role in the pathogenesis of B pertussis. We show that Czech recent isolates (2008-2015) are significantly more resistant to serum killing in vitro than the original vaccine strains (1954-1965). This phenomenon seems to result from the adaptation of global B. pertussis population to its human host. In addition, this diploma...
|
|
|
Interaction of the human pathogen Bordetella pertussis with blood serum
Štipl, Daniel ; Večerek, Branislav (advisor) ; Kamanová, Jana (referee)
Bordetella pertussis is a Gram-negative strictly human pathogen and the major causative agent of whooping cough or pertussis. The incidence of this highly contagious respiratory disease in developed countries has increased in the last decades. One of the less characterized virulence factors of B. pertussis is the type three secretion system (TTSS) which is responsible for the secretion of the effector proteins into host eukaryotic cells. This diploma thesis sheds light onto factors influencing TTSS in vitro activity. Although TTSS of laboratory strain Tohama I was induced by biologically active compounds present in blood (e. g. complement proteins), TTSS of recent clinical isolate B1917 seems to be induced permanently. Furthermore, BB0302 encoding a GntR family transcription regulator in B. bronchiseptica RB50 (homologous to BP0209 of Tohama I) was studied, however, the deletion of this gene did not affect the TTSS functionality. Serum resistance is a factor that plays a key role in the pathogenesis of B pertussis. We show that Czech recent isolates (2008-2015) are significantly more resistant to serum killing in vitro than the original vaccine strains (1954-1965). This phenomenon seems to result from the adaptation of global B. pertussis population to its human host. In addition, this diploma...
|
|
|
Sanatorium with Speleotherapy for Childern Ostrov u Macochy
Tran, Hoang Long ; Kotek, arch Jakub (referee) ; Odvárka, Antonín (advisor)
The master thesis Sanatorium with Speleotherapy for Children - Ostrov u Macochy is an architectonical concept which consist of the establishment of a sanatorium with various functions for patients and the public. The compound offers a medical block, administrative and economic blocks, a school, a cafeteria for visitors, a guests house for adult clients and complement. The building site is located on an inclined terrain near Císařské jeskyně (Caesar cave) and it is surrounded by forest from three sides. There is a scenic view to the south from the construction site. The neighboring buildings consist only of family houses. The compound of sanatorium aimed to have the closest interaction with surroundings and the terrain, therefore the estate is divided into three different segments based on the three most important functions of the construction program.
|
|
|
Complement-binding antibodies in patients after organ transplantation and their clinical relevance
Kovandová, Barbora ; Slavčev, Antonij (advisor) ; Krulová, Magdaléna (referee)
The diagnosis of antibody-mediated rejection after liver transplantation is complicated, due to the fact that the clinical and pathological signs of this life- threatening complication are often overlapping with non-immunological symptoms, like biliary obstruction, ischemia, thrombosis and others. Furthermore, the transplanted liver is to a great extent resistant to this type of rejection. Like in the transplanted kidney and heart, the main pathological factors of graft injury are antibodies directed to the mismatched HLA antigens of the organ donor, i.e. donor- specific antibodies. Besides analysis of HLA specificity of antibodies, research lately has been directed to define whether these antibodies are complement-binding or not. Literature data on this are however up till now limited. Therefore, the aim of this diploma thesis was to study the clinical relevance of complement-binding antibodies against HLA antigens in patients after liver transplantation. Our preliminary results suggest that there might be a correlation between the presence of complement-binding antibodies and the development of antibody-mediated rejection. This finding may play a role for improvement of the prognosis of patients after liver transplantation. Key words HLA, antibodies, C4d, complement, liver transplantation
|
|
|
The complement of the Spanish adjective
Koťátková, Adéla ; Čermák, Petr (advisor) ; Kratochvílová, Dana (referee)
The aim of this thesis is to analyse and describe the complement of the Spanish adjective. We clarify the heterogeneity of linguistic theories that approach this topic. We select the theory of valency in order to delineate the complement of adjective as a valence complement. We review its characteristics and compare the information in the quoted literature with the results of linguistic corpuses CREA and InterCorp. In the practical part, we analyse the corpuses for the combinations of adjective valency, obligation, the possibility of ellipsis, the influence of quantification on the complement and collocations. Key words: Spanish - linguistic corpus - InterCorp - CREA - valency - complement - adjective
|
|
|
Validation of Utility Theory of a Consumer in Practice
Kašpárková, Ivana ; Svoboda, Roman (advisor) ; Šrédl, Karel (referee)
This bachelor thesis is focused on validating of Utility Theory of a Consumer in practice. The aim of this work is to confirm or disprove the theory of decreasing marginal utility on the two test groups of respondents coresponding to a pre-prepared questionnaire.
The work is divided into two main parts first is a theoretical and second is a practical. The theoretical part is devoted to the authors, who were stadied to the issue of consumer behavior from the past until present.
These findings are used to develop questionnaires and subsequent elaboration of the practical part. In the practical part are analyzed using a graphical representation individual responses to the questionnaire from two interviewees groups. These data lead to the evaluation of whether the theory of diminishing marginal utility confirmed or not. Average utility are further processed in statistical calculations to compare whether the average utility in the two groups tested statistically significantly different or are the same average utility.
|
|
|
Citlivost spirochet Lymské boreliózy ke komplementu různých druhů zvířat chovaných v zoologických zahradách: potenciál rezervoárových hostitelů vybraných exotických obratlovců
TICHÁ, Lucie
Reaction of vertebrate serum complement with different Borrelia burgdorferi sensu lato species was used as a basis in determining reservoir hosts among domesticated and wild animals. Borrelia burgdorferi sensu stricto, Borrelia garinii and Borrelia afzelii were tested for their sensitivity to serum of exotic vertebrate species housed in 5 zoos located in the Czech Republic. We confirmed that different Borrelia species have different sensitivity to host serum. We found that different tolerance to Borrelia infection possessed by hosts might alter among the individuals of the same genera or species and is not affected by host's age or sex. From all zoo animals included in our study, carnivores demonstrated the highest apparent reservoir competency for Lyme borreliosis spirochetes.
|
|
|
Sensitivity of spirochetes from \kur{Borrelia burgdorferi} sensu lato complex to human complement: infection potential of selected species
TICHÁ, Lucie
Sensitivity of spirochetes from Borrelia burgdorferi sensu lato complex to serum complement of humans of different age and sex was analyzed. Complement-mediated Borrelia killing was observed in different combination of serum and selected Borrelia genospecies. The obtained results confirmed that age itself does not influence the sensitivity of human to Borrelia infection. However, the females seem to be more vulnerable to it. Each of ten tested Borrelia species was proved to be potentially infective for human in different ratio. The clear separation of all ten checked Borrelia species into two groups was revealed after the reaction with human sera: species with low sensitivity to human serum complement (mortality below 1 percent) and species with higher sensitivity (mortality over 3-4 percent).
|
guest ::
