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The in vitro effects of selected isoflavonoids on isolated rat aorta
Novýsedláková, Alena ; Pourová, Jana (advisor) ; Vopršalová, Marie (referee)
Charles University in Prague Faculty of Pharmacy in Hradec Králové Department of Pharmacology and Toxicology Student: Alena Novýsedláková Supervisor: PharmDr. Jana Pourová, PhD. Title of Thesis: In vitro effects od selected isoflavonoids on isolated rat aorta Abstract: The aim of this study was to determine whether the selected isoflavonoids are able to vasodilate aorta, what structural features might be responsible for a relaxing activity and estimate the mechanism of action. The experiment we carried out the classical in vitro method on the isolated rat aorta, and the results evaluated by the computer program GraphPad. Only glycitin did not show statistically significant relaxation of blood vessels, which can be attributed to ineffective glycosidic form. Other izoflavonoids- glycitein, daidzein and tectorigenin seem to be promising in terms of potential vasodilatation. Further studies would be needed to verify also the in vivo effects. Likely structural features include the hydroxy group at 7-position and a methoxy group at position 8 of the core od isoflavonoids. The mechanism of action is not yet known, but most likely appears endothelium-dependent mechanisms, alpha 1 receptor antagonism and agonism of muscarinic M3 receptor. It may be also involved in the effect of the antioxidant and...
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Secondary metabolites of plant cultures in vitro I
Zlochová, Pavlína ; Kašparová, Marie (advisor) ; Siatka, Tomáš (referee)
Charles University, Faculty of Pharmacy in Hradec Králové Department of Pharmacognosy Candidate: Pavlína Zlochová Supervisor: PharmDr. Marie Kašparová, Ph.D. Title of diploma thesis: Secondary metabolites of plant cultures in vitro I. Key words: Suspension cultures, Trifolium pratense, phytoestrogens, flavonoids, isoflavonoids Explant cultures are source of plant secondary metabolites. Nevertheless the production of secondary metabolites is usually low in explant cultures. This production can be increased by elicitation metods. Suitable elicitor is added into the cultivate medium and leads to gene expression and production of secondary metabolites. The aim of this study was to observe the influence of sodium chloride on the production of flavonoids and isoflavonoids by the Trifolium pratense L. suspension culture (Tempus variety). The culture was cultivated on Gamborg nutriet medium with addition of 2 mg.l-1 2,4- dichlorophenoxyacetic acid and 2 mg.l-1 6-benzylaminopurine. Cultivation proceed in 25řC temperature and 16 hours light/8 hours dark period. Then determination of flavonoids in according to Czech Pharmacopoeia 2009 and the determination of isoflavonoids by HPLC method was performed. The best elicitation effect on the production of flavonoids was 175 mmol.l-1 of sodium chloride after a 3...
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Metabolism of isoflavonoids in human organism
Šramarová, Pavlína ; Karlíčková, Jana (advisor) ; Jahodář, Luděk (referee)
Charles University in Prague Faculty of Pharmacy in Hradec Králové Department of Pharmaceutical Botany and Ecology Candidate: Bc. Pavlína Šramarová Diploma thesis supervisor: PharmDr. Jana Karlíčková, Ph.D. Title of thesis 2015/2016: Metabolism of isoflavonoids in human organism, pp. 54 Isoflavonoids are subgroup of flavonoids, belonging to the phytoestrogenic compounds. These compounds occur especially in leguminous, mainly in soya (Glycine max (L.) Merr). They have a positive impact on human health, especially for its estrogenic effects, due to which are applied in the fight against osteoporosis, cardiovascular diseases, atherosclerosis and for moderation of menopausal symptoms. Furthermore they exhibit antibacterial activity and protect cells against damage of DNA. This thesis deals with their metabolism and action in human and animal organism, also with the influence of intestinal microflora to transform these isoflavonoids into metabolites, as well as the effects of these metabolites. There are also mentioned these isoflavonoids: daidzein, genistein, biochanin A, formononetin, calycosin, prunetin, puerarin, cladrin, tectorigenin and kakkalid. Key words: isoflavonoids, metabolism, human organism
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Plants as source of isoflavonoids
Raabová, Karin ; Karlíčková, Jana (advisor) ; Vytlačilová, Jitka (referee)
CHARLES UNIVERSITY IN PRAGUE PHARMACEUTICAL FACULTY IN HRADEC KRÁLOVÉ DEPARTMENT OF PHARMACEUTICAL BOTANY AND ECOLOGY Title of the Bachelor thesis: PLANTS AS SOURCE OF ISOFLAVONOIDS Candidate: Karin Raabová Supervisor: PharmDr. Jana Karlíčková, Ph.D. Bachelor thesis 2014/2015, pp. 65 Isoflavonoids belong to the low molecular weight secondary metabolites of plants produced by many plant families, particularly plants of the family Fabaceae. They are a common part of the human diet (peas, beans, soybeans) and have many biological effects, such as the estrogenic and antioxidant effects, but also can exhibit larvicidal activity. To subsequent establish the presence of these substances in plants are most often used special instrumental (HPLC, MS, DAD) or immunological (ELISA, RIA) methods. This thesis is a literature review, which aimed to gather information about plant sources of isoflavonoids. Among their significant sources of the Kingdom Plantae belong for example Pueraria, Dalbergia, Astragalus, Belamcanda and Gynerium. Keywords: isoflavonoids, plants, biological activity, identification
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Secondary metabolites of plant tissue culture of Trifolium pratense L.
Novotná, Hana ; Kašparová, Marie (advisor) ; Siatka, Tomáš (referee)
Charles University in Prague, Faculty of Pharmacy in Hradec Králové Department of Pharmacognosy Candidate: Hana Novotná Supervisor: PharmDr. Marie Kašparová, Ph.D. Title of diploma thesis: Secondary metabolites of plant tissue culture of Trifolium pratense L. Explant cultures are perspective sources of secondary metabolites. Nevertheless production of flavonoids and isoflavonoids by the suspension culture of Trifolium pratense L. is not high. Elicitation is one of the methods used to enhance the biosynthesis of secondary metabolites. Elicitation induces physiological changes, stimulates defensive or stress-induced reactions in plants and subsequently triggers the synthesis of secondary metabolites. The objective of this study was to observe the influence of two elicitors - abscisic acid and ascorbic acid - on the production of flavonoids and isoflavonoids by the Trifolium pratense L. suspension culture (Sprint variety). The culture was cultivated in Gamborg medium to which 2 mg.l-1 of 2,4- dichlorophenoxyacetic acid and 2 mg.l-1 of 6-benzylaminopurine were added, at the temperature of 25 řC and 16 hours light / 8 hours dark period. The best elicitation effect of abscisic acid on the production of flavonoids and isoflavonoids was observed after a 6-hour application of the highest 500 µmol.l-1...
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Copper reducing effects of isoflavonoids
Ambrožová, Kamila ; Karlíčková, Jana (advisor) ; Martin, Jan (referee)
Charles University in Prague Faculty of Pharmacy in Hradci Králové Department of Pharmaceutical Botany and Ecology Candidate: Kamila Ambrožová Supervisor: PharmDr. Jana Karlíčková, Ph.D. Title of Thesis: Copper reducing activity of isoflavonoids Copper in our body appears only in trace amounts. Despite this fact is an important component of many enzymes, which are responsible for right function of the whole organism. The most important are antioxidant activity, collagen and conective tissue synthesis, formation of pigmentation and cell respiration. Main target of the organism is control the homeostasis of copper, because excess and deficiency of the element could be pathological. Increase level of copper can cause many of civilization diseases. Isoflavones are the plant metabolites of polyfenolic structure which can positively affect different processes in our body. Research is dedicated to anti-inflammatory and estrogenic effects, antioxidant and anticancer aktivity. The elimination and inactivation of radical elements is performed becase of ability of isoflavones to reduce transition elements or chelate them. The aim of this thesis was to determine the reducing activity of seven isoflavonoids - daidzein, genistein, biochanin A, genistin, glycitein, formononetin and prunetin. The highest reduction...
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