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Possibilities of pathogen identification using MALDI-TOF mass spectrometry
Fusková, Lucie ; Strouhalová,, Dana (referee) ; Šalplachta, Jiří (advisor)
This bachelor thesis focuses on the utilization of MALDI-TOF MS technique for the identification of plant pathogens, specifically targeting molds of the genus Fusarium affecting cereals. Molds pose a serious threat to agriculture and the food industry. Current identification methods are time-consuming and often fail to provide precise results. Mass spectrometry offers a rapid and reliable alternative. Identification via MALDI is characterized by its high accuracy, sensitivity, and speed compared to commonly used methods. The thesis addresses the optimization of the method using cultured fungal strains and the identification of molds in infected cereals. The results of the study demonstrate the potential of utilizing MALDI technique for the identification of plant pathogens.
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Methods of ignition spark gaps
Pekárek, Dominik ; Štěpánek, Jaroslav (referee) ; Krbal, Michal (advisor)
This thesis deals with methods of spark gap ignition. The thesis describes spark gap ignition by external electrode, by Rogowski electrode and by lasers. Advantages and disadvantages of these methods are also discussed. In the final part of the thesis there is described experiment with high voltage spark gap.
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Theoretical - practical view of the levitation of the lifter
Buday, Matej ; Bartlová, Milada (referee) ; Zdražil, Vladimír (advisor)
Levitation and its effects becomes a huge trend of modern physics. It is very important to be accurate and patient in the research. Representative of the theory, which we are examining, is a lifter and its levitation in electrostatic field. Air ionization is produced by high voltage connected to the lifter’s electrodes and a beam of plasma will surround a thin wire electrode. The levitation effect will appear, if there is right magnitude of electric field intensity and right method of lifter construction.
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Optimization of the Colutron Ion Source
Horký, Michal ; Mach, Jindřich (referee) ; Voborný, Stanislav (advisor)
This bachelor’s thesis concerns with design and assembly of Colutron ion source which produces beam of nitrogen ions with energy from tens to thousand eV. Production of ions and the principle of Colutron ion source is described in the first part. The second part of the thesis is related to the modification and assembly of commercial Colutron ion source. Finally the parameters of the ion beam have been measured for different extraction electrodes and operating parameters in testing apparatus.
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Air ion measurement
Lazorka, Martin ; Roubal, Zdeněk (referee) ; Špinka, Jiří (advisor)
This thesis focuses on the basic problems of ions and the ionization itself, what influence ions have to the organism. Further it examines compensation of small negative ions in the residential environment. Introduction to measurement of ion concentrations. First measurements with the aspiration capacitor which is now the most commonly used measurement of ion fields. Measurement is focused on the concentration of air ions in the building, VUT FEKT T-10, then the concentration changes depending on the daily cycle, ventilation and concentration before teaching and after teaching.
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Development of DC arc quenching chamber
Černohorský, Petr ; Valenta, Jiří (referee) ; Šimek, David (advisor)
Seminar thesis focuses on the issues about quenching the electric direct arc by the quanching system. Teoretical part deals with action about quanching the direct arc and with another actions which happens in direc discharge. There is described proposal and construction of the insulating quenching system. Than chamber was testing in high voltage laboratory and the results from testing was evaluated.
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Design of Portable Air Purifier
Žerávková, Iveta ; Buganská, Tamara (referee) ; Sovják, Richard (advisor)
The topic of my Bachelor's project is focused on the design of a air purifier. Specifically product with a content of up to 500 m3 / h and a power consumption of 65 W. I must use materials from the FSI Science fund 2016. The aim of the design is to design a functional model with respect to technical, graphic, ergonomic and especially material requirements.
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Design and development of the electrostatic precipitator
Kvasnička, Karel ; Radil, Lukáš (referee) ; Ptáček, Michal (advisor)
This bachelor thesis deals with the design of an electrostatic precipitator and familiarization with the problems of separation on these separators. The aim of this work is to construct a functional model of an electrostatic precipitator. The principle of operation and design will be described. In addition, the design of a separator is elaborated, which was subsequently realized and experimentally verified in the CVVOZE Power Laboratories Research Infrastructure. The first part deals with the description of the legislation on air protection, the most frequent input material of separators and descriptions of the basic types of separators. In addition, the electric separator, its area of use, construction and individual components, as well as the undesirable operating state of the separator, are further elaborated. In the second part are presented the equations and calculations for the design of the separator. In the next part, the separator designer and his laboratory tests are described. The separator was subjected to basic voltage tests from the viewpoint of its operating voltage. Its functionality fits the realized experiments up to 30 kV. On the basis of this work and the production of the equipment, it is possible in the future to test the properties of flue gases and other gases.
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Mass Spectrometry
Smejkalová, Tereza ; Baumgartnerová, Alena (referee) ; Krhutová, Milena (advisor)
Hmotnostní spektrometrie je metoda analytické chemie používaná k analýze organických i anorganických sloučenin. O tomto tématu se běžně dozvídáme z prací týkajících se chemie, protože tato metoda může být využita k identifikaci mnoha druhů sloučenin. Vlastní fungování přístrojů používaných v hmotnostní spektrometrii ale musíme hledat ve specializovaných knihách zaměřených na tuto metodu a tyto informace jsou obvykle popsané velmi komplikovaně. Proto jsem se rozhodla napsat práci, která uvádí základní informace o hmotnostní spektrometrii z pohledu elektrotechniky, srozumitelně popisuje jednotlivé fáze této metody a která nebude zaměřena na výstup analýzy, ale na technický proces. V druhé části práce se věnuji praktickému použití hmotnostních spektrometrů v průmyslu při testování těsnosti produktů a typům testování těsnosti, vysvětluji důležité termíny a detailně popisuji spektrometr LDS3000. Mým záměrem je ukázat, že tato metoda je skutečně zajímavá a poutavá a chci ji proto představit ostatním.
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The influence of air ionization on the laser beam propagation
Fučík, Milan ; Kubásek, Radek (referee) ; Drexler, Petr (advisor)
The semester project deals with the mechanisms of propagation of electromagnetic wave in ionized environment. The focus is placed on the propagation of light in ionized air. The air ionization influences its properties in the view of its material parameter – permittivity. The change of air permittivity results in the light speed reduction. The theoretical analysis of light propagation is carried out in the work in the view of impact on the light speed. The light speed reduction in ionized air is experimentally verified. The conclusions on the practical utilization of this ionization measurement method are given.
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