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GENE EXPRESSION AND IMMUNOLOGICAL RESPONSE IN MICE EXPOSED TO ZnO NANOPARTICLES
Rössner ml., Pavel ; Vrbová, Kristýna ; Strapáčová, S. ; Rössnerová, Andrea ; Ambrož, Antonín ; Brzicová, Táňa ; Líbalová, Helena ; Javorková, Eliška ; Zajícová, Alena ; Holáň, Vladimír ; Kulich, P. ; Večeřa, Zbyněk ; Mikuška, Pavel ; Coufalík, Pavel ; Křůmal, Kamil ; Čapka, Lukáš ; Dočekal, Bohumil ; Šerý, Omar ; Machala, M. ; Topinka, Jan
We analyzed gene expression changes in the lungs and the immunological response in splenocytes of mice exposed by inhalation of ZnO nanoparticles - NP. Adult female ICR mice were treated for three days and three months, respectively. Analysis of differential expression in genes involved in oxidative stress was conducted using quantitative RT-PCR. The potential immunotoxic and immunomodulatory effects of ZnO NP were analyzed by phenotyping and cytokine production by splenocytes after three months exposure. Three days exposure resulted in down-regulation of GCLC, GSR, HMOX-1, NQO-1, NF-kB2, PTGS2 and TXNRD1 mRNA expression, three months exposure increased the expression of these genes. Three months exposure caused a significant decrease in the percentage of granulocytes in the spleen cells, and affected the production of IL-10 and IL-6 by lipopolysaccharide-stimulated leukocytes. In summary, our study revealed changes in the expression of genes involved in the oxidative stress response following acute ZnO NP exposure. Subchronic ZnO NP exposure induced immunomodulatory effects in the spleen.
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Effect of inhaled metal oxide nanoparticles on organs of exposed mice
Mikuška, Pavel ; Večeřa, Zbyněk ; Dočekal, Bohumil ; Čapka, Lukáš ; Křůmal, Kamil ; Coufalík, Pavel ; Moravec, Pavel ; Buchtová, Marcela ; Míšek, Ivan ; Vrlíková, Lucie ; Smutná, Tereza ; Dumková, J. ; Hampl, A. ; Fictum, P.
Adult mice were continuously exposed to metal oxide nanoparticles for different time intervals. At the end of experiment, mice organs (lung, brain, kidney, liver and spleen) were collected for chemical, biochemical, histological and electron microscopical analyses.
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Old - new urban spa
Hrušková, Ludmila ; Šindlar, Jiljí (referee) ; Hrabec, Josef (advisor)
The bachelor thesis deals with the town spa in Zábrdovice by architect Bohuslav Fuchs. Spaces were inserted into the spa, which did not burden their construction with their functions. The urbanistic study deals with the Zábrdovice area, which will become the Military Campus.
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Study of organs of mice in inhalation experiments
Vrlíková, Lucie ; Diviš, Pavel (referee) ; Dočekal, Bohumil (advisor)
The diploma thesis is devoted to study potential risks for living organisms resulting from inhalation of nanoparticles of chosen heavy metals. The main aim of this work is the investigation of organs from experimental white mice after their guided inhalation of lead nanoparticles. The samples of liver and lungs from these animals are taken in time periods during the inhalation experiments and processed by specific methods for their evaluation. After pressurized microwave assisted decomposition of selected samples of mice organs in high purity concentrated nitric acid the content of lead is determined by atomic absorption spectrometry with electrothermal atomization. Furthermore, the changes are monitored using the microscopy of histological slides stained with hematoxylin-eosin. The results of both approaches are evaluated and compared within the frame of this work.
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Influence of PbO and CdO nanoparticles on particular physiological functions in mouse.
Svozilová, Eva ; Vrlíková, Lucie (referee) ; Večeřa, Zbyněk (advisor)
The aim of this bachelor´s thesis is to assess the long-term effects of inhalation of nanoparticles of lead oxide and cadmium oxide on the weight of selected organs of experimental white mice. The selected organs (spleen, liver, kidney, lungs, brain) were successively collected during a period of thirteen weeks. The effect of inhalation of both metal oxides was statistically evaluated. In both study groups of the experiment (PbO and CdO), the relation between organs weight and the length of inhalation and the relation between organs weight and inhalation of differing metal concentrations were evaluated, and results both of the study groups were compared to each other.
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Inhalation of nanoparticles MnxOy
Mikuška, Pavel ; Večeřa, Zbyněk ; Dočekal, Bohumil
Contribution deals with inhalation of nanoparticles MnxOy by small experimental animals and with study of Mn allocation in organs of exposed mice. Content of Mn in organs of exposed mice was compared with content of Mn in organs of control mice that were not exposed to inhalation of MnxOy nanoparticles.
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Active Particles at the Temelín NPP, Radiobiological Risks and Protective Precautions
KAŇKOVSKÝ, Josef
Active Particles at the Temelin NPP, Radiobiological Risks and Protective Precautions The term active particle (AC) was applied on Temelín NPP (ETE) in order to denominate small fragments of high radioactive matters, sized up to 1 millimeter, forming into primary circuit. In accordance with latest available know-how, the ACs major contains corrosion products, that were activated during passing through reactor core. After primary circuit opening, due to carry out outage works, the ACs will spread into ETE radiation controlled area. In proportion to their size, the ACs activity is relative high, so that the ACs can jeopard workers, who will contact them. This jeopardy is namely associated with AC penetration into organism - ingestion or inhalation. The main goal of this dissertation is to determine grounds of ACs occurence in Temelín NPP, to review ACs radiobiological risks and to evaluate procedures and protective aids, used for assurance of workers radiation protection. This dissertation is resuming actual know-how about ACs occured and occuring in Temelín NPP, including suggestions for radiation protection procedures and for protective aids utilization, that are to be used for reduction of above mentioned jeopardy. Next areas are concerned: - analysis of ACs forming and matters composition - description of ACs physical-radiation parameters - identification of main ACs sources in primary circuit - assesment and evaluation of radiobiological jeopardies, associated with ACs occurence in Temelín NPP radiation controlled area - assesment of procedures and protective aids used for protection of workers, who can be endangered by ACs
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The role of a nurse in patiens with inserted endobronchial stent.
PEŠKOVÁ, Martina
The goal of this bachelor thesis was to ascertain what role the nurse performs in care for patient with inserted endobronchial stent. The research set consists of ten patients with stenosis of trachea and large airways. Stenosis has a lot of causes but all patients have similar symptoms. The basic problem of such patients stays the same; they come because of breathlessness that restricts them in performance of common daily activities. We asked the research question whether the nursing care for patient with inserted endobronchial stent has influence on improvement of the breathlessness and whether education by the nurse is important for the patient with inserted endobronchial stent. The goal was met and the research questions answered. Three hypotheses were set on the base of the result. H1: Insertion of endobronchial stent has influence on improvement of patient{\crq}s breathlessness. H2: Nursing care for patient with inserted endobronchial stent has influence on self-care in patient{\crq}s daily activities. H3: The most important topic of education in patient with inserted endobronchial stent is inhalation. The nurse{\crq}s nursing care is indispensable because the patient needs it for the whole period of the difficulties. The influence of nursing care improves the patient{\crq}s discomfort caused by breathlessness. The nurse{\crq}s care provides the patients with physical, but also with psychic support. The patients admitted because of breathlessness are tired, exhausted and completely dependent on oxygen. They are not able to perform sanitary needs by themselves, are also restricted in movement, feeding, but also in dressing and evacuation. The nurse chooses, individually and by the patients{\crq} possibilities, such techniques that allow satisfaction of all bio-psycho-social needs during provision of nursing care. She provides also psychic support important to reach the goal, i.e. to eliminate the patients{\crq} breathlessness. Not all patients master the breathlessness well; there are patients who need psychic support by the nurse. We also describe the significance of education by the nurse in this bachelor thesis. The role of the nurse is to perform education so that the patients understand that daily hygiene of airways is, because of the inserted endobronchial stent, very important and has its role. Insufficient nurse{\crq}s education and therefore bad hygiene of airways causes complications because of which the patient must seek medical attention and be hospitalized. This bachelor thesis will serve also in practice to familiarize the general public at lay and professional level with the a possibility of therapy of breathlessness of patients with stenosis in large airways.
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