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Vliv stupně příbuzenské plemenitby na reprodukční výkonnost ovcí plemene Zwartbles
Pospíšil, Jakub
The bachelor thesis is focused on the influence of inbreeding degree on reproductive performance in Zwartbles sheep. A brief description of the Zwartbles breed, its history and performance is given in the literature search. Furthermore, a general description of reproduction and the evaluation of reproductive performance. Finally, the thesis discusses breeding and the determination of the degree of inbreeding. The practical part of the bachelor thesis is focused on the analysis of the influence of inbreeding on the reproductive performance of the Zwartbles herd bred on the Mohelno sheep farm in 2006 - 2021. The herd was divided according to the degree of inbreeding into four groups. For ewes, the percentage of born and weaned pups was evaluated. For lambs, gain, height of the longest lumbar muscle and chest (MLLT), loin fat height, conformation, and weight of lambs at 100 days of age were evaluated.
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Populační a ochranářská genetika rysa ostrovida na okraji Západních Karpat
Stehlíková, Monika
The diploma thesis deals with the population and conservation genetics of the Eurasian lynx on the edge of the Western Carpathians, including the territory of the Beskydy Protected Landscape Area on the Czech side and the Kysuce Protected Landscape Area on the Slovak side (total ?1500 km2). The aim of the study was to evaluate using non-invasive genetic samples the temporal changes of the genetic diversity and abundance of this marginal lynx subpopulation inhabiting the Moravskoslezské Beskydy Mountains, Vsetínské Beskydy Mountains and Javorníky Mountains during the last five seasons from 2016/17 to 2020/21. Relatedness and spatial activity of selected individuals were also evaluated. The obtained genotypes were supplemented with previously genotyped individuals. In total, 37 individuals (12 females and 25 males) were detected over the period of five seasons. The estimated mean population size was 14 individuals and the effective population size varied between 3.7–7 individuals. The obtained results demonstrate the gradually decreasing genetic diversity of this subpopulation and increasing inbreeding; the inbreeding coefficient in the last season of 2020/21 recorded the highest value of FIS= 0.169. The results also showed that individuals actively reproduced there, at least 15 offspring were identified during this period, some of them settled and reproduced in the area. The spatial activity of the lynx confirmed the interconnectedness of the mountain ranges and the connection of this subpopulation to the West-Carpathian population, which is confirmed by the arrival of several unrelated individuals. In the monitored period, four individuals were also recorded to disperse westwards outside the study area, but all except of one died due to a collision with a vehicle.
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Conservation genetics of Galápagos mockingbirds: from immune genes to genomes
VLČEK, Jakub
In this thesis I have dealt with the population genetic processes of mockingbirds in the Galápagos Islands ( Mimus ) in relation to the limited island area, from the perspective of two types of immune genes and the genome-wide approach. The thesis starts with an introduction to population genetic concepts relevant to conservation genetics followed by description of immune genes: the major histocompatibility complex (MHC) and the Toll-like receptors (TLR). In the final part of the introduction, I address how genetic drift, selection and inbreeding shape genome-wide genetic patterns in small populations. The introduction is followed by four chapters, beginning with an examination of MHCII polymorphism in populations of mockingbirds in the Galápagos Islands. The study of the relationship between MHCII and the abundance of ectoparasites is the content of the second chapter. The third chapter shows how polymorphism of TLRs is shaped by interaction of genetic drift and natural selection. The last chapter deals with the effects of limited island size on inbreeding and genetic load, supported by inferences of the past demography of mockingbirds. The thesis concludes with a summary of the results and their potential implications in the field of conservation genetics.
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Possibilities of ex situ protection of rare and endangered plant species
Vítová, Jana ; Münzbergová, Zuzana (advisor) ; Dostálek, Tomáš (referee)
The aim of the bachelor's thesis is to present the possibilities, significance and possible problems of ex-situ protection for the future conservation of biodiversity of plants on Earth. In the introductory part of the thesis, the individual processes and programs of ex situ protection are described, with the help of literature search. At the same time, information on international cooperation in the field of seed banks and botanical gardens is presented, which, through their activities and mutual cooperation, create rescue programs to secure as many samples of plant material for possible future use. The work also mentions international plant databases, which provide the accurate and detailed information about the preserved material of rare and endangered plant species for the public. Further in the thesis, the currently known problems of ex situ protection with their impacts on the collected and stored material are mapped. Keywords Ex situ conservation, threatened plants, Ministry of the Environment, database, seed dormancy, inbreeding, outbreed depression, cultivation, seed bank, botanical gardens, tissue cultures, in vitro, genetic drift, mutation, hybridization, wildlife conservation, endangered species, threatened species
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Canaan Dog and Dog Assistant Education
Rutová, Šárka ; Jančaříková, Kateřina (advisor) ; Pavlasová, Lenka (referee)
The topic of this thesis is Canaan dog including dog assistant education. The first part is theoretical and introduces the reader to the Canaan dog breed. The origin of this rare and very old breed is Izrael and first dogs were imported to the Europe and America in 60s of 20th century. The lack of Canaan dogs in Europe causes problems with breeding and transmission of genetic diseases. Especially in the Czech republic there is only few dogs. The teoretical part introduces a transmission of genetic diseases and other diseases. The practical part is focused on a creation of a genealogy of Canaan dogs in the Czech republic. Further I studied inbreeding. In the second part I studied the use of dog in education. The practical part are two education programs, which I created. The first training program is about dogs behaviour, it's also tested in practice and its completed results are part of the appendix. Pupils will learn important information about dogs, how to handle and understand a dog or information about a primitive breed like rare Canaan dog. The second education program is designed for high school students. The program is about inbreeding and includes text and worksheet. Key words: Canaan dog, genetic, heritage, genetic diseases of dogs, inbreeding, animal assistant education.
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Evolution of a dog domestication and genetic diversity of the recent breeds.
Ungrová, Lenka ; Černá Bolfíková, Barbora (advisor) ; Vinkler, Michal (referee)
The origin, timing and the developement of the first stages of domestication are the most frequently studied topics within the evolutionary domestication. Even there are a lot of studies, there are also a lot question that have not been answered. Consequent processes are not less interesting. It is known that the first prototypes of dog breeds were formed much earlier before the first breeding clubs were established. We can say, that the first selection was for the type of work which should be carried by the dog, besides the selection for tameness. The next step of selection included selection for the specific phenotypic traits and often involved targeted hybridization between emerging breeds. Over the past 200 years, more than 400 breeds with a unique combination of traits were recognized. This thesis aims to map the emergence of modern breeds from a genetic point of view. Thesis also summarizes the effect of evolution and domestication on specific traits and genes and how they affected the genetic diversity of dog breeds.
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Inbreeging and its influence on the fitness of animal bread in captivity
Králová, Michala ; Frynta, Daniel (advisor) ; Komárková, Martina (referee)
Habitat loss and fragmentation is now the current problem, which leads to an inevitable reduction in the effective size of populations of many species. The negative effect of reduction further enhance processes such as genetic drift and inbreeding that causes deterioration of fitness (tj.inbreeding depression). Inbreeding and related phenomena may therefore be a threat to the survival of remaining populations of endangered species. Negative effects of inbreeding on population held in captivity that are part of the various emergency programs is even more recent concern (Frankham et al. 2002, O'Grady 2006). The aim of this study was to evaluate the current literature on the effects of inbreeding on populations of fish, reptiles, birds and mammals. Inbreeding affects mainly qualities associated with fitness and sexual selection traits and behavior.
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Genetic variability in the European ground squirrel (Spermophilus citellus) in Czech Republic
Starcová, Magda ; Vohralík, Vladimír (advisor) ; Kotlík, Petr (referee)
The European ground squirrel (Spermophilus citellus) (hereinafter EGS) is under the Law on Nature Protection and is included as a critically endangered into the Red List of Endangered Species. In the Czech Republic, which represents the northwestern border of the species range, EGS currently occurs on 34 localities, which are relatively small and isolated from each other. The spread of this species to central Europe was connected with neolithic deforestation. Disjunctive type of distribution of EGS was caused by strong decline of its abundance during the second half of the 20th century due to changes in field management, fragmentation of landscape and other factors. It can be expected that this process left traces on the genetic structure of EGS populations. The major objective of this study was a detailed analysis of genetic variability of EGS populations in the Czech Republic and searching for corelations with available data about its biology and demography. It was used 13 microsatellite loci and in total were processed 408 samples from 27 localities in CZ and 3 samples from one Hungarian locality as an outgroup. With aid of various methods, details of substructure and diferentiation of individual populations, genetic variability, degree of inbreeding and geographic distribution of genetic variability...
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Genetic structure of local domestic breeds
Neradilová, Silvie ; Baranyiová, Eva (advisor)
A switch from hunting to beginnings of farming and agriculture was a crucial step for humans and for developing civilization. This period was suitable for domestication processes. Dogs were the first domesticated animals at all. Their breeding was connected with settlement and cattle protection and they were used as shepherd and hunting dogs. In some cultures they are even used as food source. Recent trends lead to breeding of new breeds and division of original gene pool into smaller closed groups which lead to an increase of homozygosity and to display recessively inherited diseases. These effects are mostly visible in small local breeds that have a small population size. Global protection of genetic diversity in cattle, poultry and pigs was already addressed in many studies, probably because of the high economic value of these animals. Impact of domesticated carnivores to humans is comparable and due to the growing market in veterinary services it is needed to protect sufficient genetic variability of various unique races. The aim of this work is to compare selected local breeds of dogs from different regions of origin, to evaluate their overall genetic diversity, to determine the occurrence of candidate types of diseases and to compare the frequency of these diseases in selected breeds. Main output is to recommend appropriate methods to protect the gene pool of these unique races. Central European breed (e.g. Český Fousek), subtropical breed (e.g. Sarplaninec), tropical continental breed (eg. Basenji), tropical island breed (e.g. The New Guinea Singing Dog) will be selected. The material will be collected at exhibitions and in cooperation with local farmers. The samples will be processed by classical methods of population genetics in the laboratory of molecular genetics FTZ ČZU.
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Populatio analysis sheep breeds included in the gene resources in the Czech republic
Benešová, Jaroslava ; Vostrý, Luboš (advisor) ; Karel, Karel (referee)
Genetic variability reflects the degree of diversity of the population breeds of livestock and seed objectives can greatly decrease. As a result of the reduction of the genetic variability of the population experiencing adverse effects or to the complete extinction of the breed. Adverse effects are manifested primarily on the fitness of animals and their commercial properties. When sufficient population size and when are these influences mating panmictic minimum, however, in populations where there is a reduction in the number of individuals, these effects are essential. In connection with inbreedingem is acting negatively influences called inbred depression. The degree of inbreeding in populations is necessary to monitor and on the basis of creating mating plans, so that was the greatest genetic gain achieved in the conservation of genetic variability, which will have a negative effect on fitness and utility properties.
Population analysis was prepared based on data from the herd-books in 1985 up to 2013. The data contained the pedigree of the Bohemian Forest sheep, which is placed between the genetic resources in the Czech Republic. Using the program SAS 5.8 (Statistical Analysis Software) the value of the coefficient of inbreeding have been established for each individual and the average value of the entire population. The other trend was the evaluation of the parameters of the vyhodnocenými coefficient of inbreeding in the reference period and the effective population size, and the generational interval for the four paths of transmission of genes and two transmission paths of genes. The trend of development of the coefficient of inbreeding (Fx) was evaluated as a kolísavě rising with an average of around 0.6%. The average generation interval (L) for the four paths of gene transfer was calculated for two years and 3.83 journey 3.55 years gene transfer.
The value of the generation interval, the shorter the period considered, resulting in the intensification of genetic gain. The effective population size (Ne) was estimated in two ways. The total effective population size (Ne) taking into account the kinship was estimated to number of 85 individuals and effective population size involving only males and females was estimated at 3070 individuals. On the basis of the analysis of the coefficient of inbreeding, the generation interval and effective size of the population it is possible to sort the sheep from the Šumava with the low degree of coefficient of inbreeding and the average generation interval. On the basis of the value of effective population size 85 individuals may be experiencing the sheep breed known as endangered.
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