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The influence of thyroid hormones and adrenal glands on mental function
Augustinová, Jana ; Šivicová, Gabriela (advisor) ; Špinka, Marek (referee)
This thesis focuses on effects of thyroid and adrenal hormones on mental functions, especially on cognitive functions and emotions. In the case of adrenal hormones it will also focus on stress and burnout syndrome, which are not considered as mental functions, but are connected to both psyche and the above mentioned hormones. The text is divided into two parts. The first, theoretical part deals with the anatomy and histology of these two organs, hormones that they produce and their influence on overall physical condition and pathology. The main section of this theoretical part deals with the description of influence of thyroid hormones and adrenal glands on the above mentioned mental functions. This work is based on both earlier and contemporary research, but predominantly on foreign research. In the second, empirical part, a proposal for longitudinal study of cortisol and thyroxine levels during depression treatment is presented. Klíčová slova: Hormones, thyroid glang, adrenal gland, mental functions, cognitive functions, emotions, stress, cortisol, thyroxine
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The stress response to cardiac catheterisation. The concentration of stress markers of the hypothalamic-pituitary-adrenal axis
Skarlandtová, Hana ; Kittnar, Otomar (advisor) ; Gregor, Pavel (referee) ; Svoboda, Miroslav (referee)
In our study, we analyzed four stress markers (cortisol, cortisone, DHEA and DHEAS) in blood serum in young sows using minimally invasive heart catheterisation as the stress factor. The marker levels were assessed in four defined periods of the experiment, beginning with the baseline level on the day before intervention (1), the second period was after the introduction of anaesthesia (2), the third was after conducting tissue stimulation or ablation (3), and the final period was after the end of the catheterisation (4). Cortisol and cortisone were detected using HPLC method, DHEA(S) by commercial kits. For statistical analyses non-parametric tests were used (due to non standard Gaussian data distribution). In our study we arranged these experiments: 1. Diurnal variability in these markers concentration during heart catheterisation was tested. 2. Are there differences between stress markers concentration the day before experiment (sampling 1) and in the day of the catheterisation (samplings 2, 3, 4)? 3. Are there differences between these markers concentration during operation (the first sampling was excluded)? 4. The cortisol/cortisone ratio was calculated. We found only minimal statistical differences in studied markers between the morning and afternoon group (p > 0.05) in experiment 1. For tested...
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Physiologic and pathophysiologic aspects of selected endocrinopathies. Their relationship to adipose tissue matebolism and insulin resistance
Ďurovcová, Viktória ; Kršek, Michal (advisor) ; Saudek, František (referee) ; Štich, Vladimír (referee)
The pathogenesis of insulin resistance is a complex and still intensively studied issue. Endocrine and paracrine activity of the adipose tissue together with mi- tochondrial dysfunction are the most discussed potential factors included in the development of insulin resistance. In the first part of our study we examined the involvement of the adipose tissue and its secretory products in the etiopathogenesis of insulin resistance in patients with Cushing's syndrome, acromegaly and simple obesity. We focused on three important regulators of metabolic homeostasis - fibroblast growth factors 21 and 19 (FGF-21 and FGF-19) and adipocyte fatty acid binding protein (FABP-4). We found significantly elevated circulating levels of FGF-21 and FABP-4 ac- companying insulin resistance in both patients with simple obesity and patients with obesity connected to Cushing's syndrome, as compared to healthy controls. The concentrations of both substances were comparable between hypercortisolic and obese patients. This finding together with the absence of correlation be- tween the levels of FGF-21 resp. FABP-4 and cortisol suggest that the reason for elevation of their concentrations is obesity and its metabolic consequences themselves rather then the effect of hypercortisolism on FGF-21 and FABP-4 production. We found no...
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Use of cell phone in liquids analysis
Křístek, Tomáš ; Sekora, Jiří (referee) ; Čmiel, Vratislav (advisor)
Technological development, broad availability and ubiquity of mobile phones made it possible in recent years to start a progression leading to the integration of mobile phones into biomedical analytical methods, the development of telemedicine and mobile personal health applications. This paper deals with the literature review of the mobile phone usage in biomedicine, with emphasis on the methods of optical analysis and technical solutions using 3D printing technology. It provides a draft and practical implementation of salivary cortiol level measuring system using a mobile phone, ELISA analytical methods, 3D printing technology and a set of custom Matlab functions connected with Matlab Mobile application and Mathworks Cloud. Also provides critical evaluation of results and method possibilities, and presents set of proposals and findings for quality of measurment optimization.
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Use of cell phone in liquids analysis
Křístek, Tomáš ; Sekora, Jiří (referee) ; Čmiel, Vratislav (advisor)
Technological development, broad availability and ubiquity of mobile phones made it possible in recent years to start a progression leading to the integration of mobile phones into biomedical analytical methods, the development of telemedicine and mobile personal health applications. This paper deals with the literature review of the mobile phone usage in biomedicine, with emphasis on the methods of optical analysis and technical solutions using 3D printing technology. It provides a draft and practical implementation of salivary cortiol level measuring system using a mobile phone, ELISA analytical methods, 3D printing technology and a set of custom Matlab functions connected with Matlab Mobile application and Mathworks Cloud. Also provides critical evaluation of results and method possibilities, and presents set of proposals and findings for quality of measurment optimization.
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Electromyography, measurement of muscle electric activity
Kořínek, Peter ; Provazník, Ivo (referee) ; Kolářová, Jana (advisor)
The task of this thesis was to become acquainted with the issue of measuring of muscle electric activity.In the theoretical part we became familiar with the computer measuringsystem BIOPAC and its options to measure monitored magnitudes. This continue with practical part, in which we watch the problematic according to a theory in a group of people. We learned data with BIOPAC according to protocol of the result of physicaleffort influence on EMG. In the end the results were statistically analysed. The data as a product offallowing research were verified by hypothesis. In thelast step, the data are presented in graphical user interface development environment. After loading of the EMG of measured person, the process are displayed-without, with and after the power with onus. It is possible to choose from different analyses.
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Impact of long-term storage on fish welfare
PECHER, Petr
The literature review is completely described by the issue of fish welfare and whether fish feel pain. Then there are describes the basic procedures for measuring fish welfare. Among which belongs for example a cortisol and glucose in fish blood capillary. The aim of the study was to evaluate the effect of different stress factors during storage on fish welfare and to changes in production, exterior and length and weight indicators. For the experiment was selected carp (Cyprinus carpio) divided into two groups by weight 2.5 kg to 2.5 kg and above. The results are the values of visceral fat, capillary blood lactate and changes in production, exterior and length-weight indicators during the application and the end of the experiment.
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Risk preferences under acute stress
Cingl, L. ; Cahlíková, Jana
Many important decisions are made under stress and they often involve risky alternatives. There has been ample evidence that stress impairs decision making in cognitive as well as in affective domains, but still very little is known about whether individual attitudes to risk change with exposure to acute stress. To directly evaluate the causal effect of stress on risk attitudes, we adopt an experimental approach in which we randomly expose participants to a psychosocial stressor in the form of a standard laboratory stress-induction procedure: the Trier Social Stress Test for Groups. Risk preferences are elicited using an incentive compatible task, which has been previously shown to predict risk-oriented behavior out of the laboratory.
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Fish Welfare in Aquaculture.
MORAVEC, Pavel
Welfare of fish in aquaculture is a review. This study is focused on full evaluation of the welfare. In the first part of the study is dedicated to the welfare expression and to the gradual historic development of the problems. In next parts, the study deals with the fish welfare, which has been rapidly developed recently. The fundamental question for the welfare of fish is disussed - if fish can feel pain and suffering. Next chapter turns attention to stress, which serves as one of the indicators for the welfare quality. The thesis sums basic ways of fish welfare measurement among which belongs, for example, a cortisol and glucose coefficient in fish blood, which is changing due to the size of stress factors. There are mentioned some different approaches to measuring the welfare. A large part of the thesis is devoted to the potential places of threat to welfare. There are described critical points in fish handling and transportation. This study also analyzes water quality, stocking density and killing fish. The last part is devoted to the welfare of fish in different systems. Welfare of fish is here observed in different areas such as sport fishing, aquaculture, fish in aquariums, scientific research and fish in open waters. The end of the thesis provides some suggestions for further scientific research into fish welfare.
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Personality, social hierarchy and hormones in primates
KONEČNÁ, Martina
This thesis deals with two main issues: personality (stable individual differences in behavior) and behavioral endocrinology (or socioendocrinology) in nonhuman primates. The first part of the thesis comprises of two primate personality studies of two species: Hanuman langurs and Barbary macaques. Two basic methods of animal personality research (behavioral coding and trait rating) were compared. Stability of personality assessments has been demonstrated. Social rank of individuals was used to validate the questionnaire ratings as well as to illustrate the independence and stability of personality assessment. The second part of the thesis is based on two studies investigating hormone levels in female Barbary macaques. The relationship between cortisol, testosterone, dominance hierarchy and behavior was investigated. And the possible effect of maternal hormone levels around the time of conception on the sex of an infant was evaluated.
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