|
|
Study of disassembly/reassembly mechanisms of ferritin protein cages and their utilization in nanomedicine
Krausová, Kateřina ; Fohlerová, Zdenka (referee) ; Heger, Zbyněk (advisor)
Diploma thesis deals with the study of dissociation and reassociation of ferritin protein cages and their use in nanomedicine. Most studies that are focused on targeted transport of pharmaceuticals using ferritin cages work with horse spleen ferritin. It is, however, its origin, which leads to increasingly frequent questions about possible immunogenicity in the patient's organism, which also provides the main motivation to test the possibility of encapsulation of low-molecular drugs into ferritins originating from alternative organisms. In the practical part the method for the study of dissociation was experimentally designed. Native polyacrylamide gel electrophoresis was used to study dissociation of equine ferritin composed of different subunit, human ferritin, and archeal Pyrococcus furiosus ferritin. The obtained subunit dissociation results were used to encapsulate the low molecular chemotherapeutic drug doxorubicin and for further characterization of the ferritin-doxorubicin complex. The efficacy of the designed nanoformulations has been verified in the treatment of malignant breast cancer. Human ferritin proves to be the optimal one. Its composition of heavy subunits corresponds to a lower protein stability, thus a more efficient opening of the structure and consequent encapsulation of the cytostatics occurs. With its 60% encapsulation efficiency of doxorubicin, low polydispersity index, effective cytotoxicity of ferritin-doxorubicin complex and minimal risk of immune response to the patient's organism, human ferritin achieves better results than commonly used horse spleen ferritin.
|
|
|
Vliv expozice těžkých kovů na expresi mRNA isoforem metalothioneinu u adherentních buněčných linií
Jordanová, Lucie
Metallothionein is a small cysteine-rich protein that plays an important role in metal homeostasis and protection against heavy metal toxicity, DNA damage and oxidative stress. This bachelor thesis was conducted to investigate the effect of heavy metal exposure on the mRNA expression of metallothionein isoforms in adherent cell lines. The effect of zinc exposure in the form of ZnSO4 on the mRNA expression of MT-1A, MT-1X and MT-2A isoforms in adherent breast cancer cell lines MCF-7, MDA-MB-231 and T-47D was investigated. Using MTT viability assay, the cell viability changes at different ZnSO4 concentrations and RNA integrity is verified using denaturing agarose gel. Polymerase chain reaction combined with reverse transcription qRT-PCR is used to study mRNA levels.
|
|
|
Traits of the "cancer-prone personality" in women with breast cancer
Svatošová, Ludmila ; Šivicová, Gabriela (advisor) ; Hrachovinová, Tamara (referee)
The thesis is dedicated to the psychosocial aspects of women with breast cancer, such as stressful events, coping strategies, social support and personal and emotional factors and their possible specificity for this group of women. Aim of the theoretical part is a summary of the findings of type C personality, personality disposed to cancer and the overview of researches and particular results. The empirical part of the thesis is based on retrospective quantitative research with a focus on the number of stressful events, coping strategies, social support and features of alexithymia in women diagnosed with breast cancer (N = 70). These results were compared with a control group of women whose cancer have not been diagnosed (N = 81). The results show a significant difference in the amount of stressful events. Namely women with breast cancer have demonstrated significantly more stressful events prior to diagnosis than women without cancer. In addition, a significant difference has been found in the use of negative coping strategies which women without cancer experience using negative coping strategies more frequently than women with breast cancer. A significant difference has been found in the use of strategy "self-aggrandizement by comparison with others" which women with breast cancer use more than...
|
|
|
The role of iron in the regulation of proteins connected with tamoxifen-resistance
Potomová, Petra ; Truksa, Jaroslav (advisor) ; Balušíková, Kamila (referee)
Cancer cells are highly dependent on nutrient uptake to sustain their increased proliferation, one of these nutrients being iron. In recent years, a heightened dependency on iron was observed in cancer cells, allowing for the proper function of numerous enzymes, DNA synthesis and mitochondrial respiration. Here, we further delve into the iron metabolism of malignant cells, attempting to understand the differences between tamoxifen-sensitive and resistant (Tam5R) ones using two breast cancer cell lines of luminal A origin, MCF7 and T47D. These cells show numerous changes in iron homeostasis and iron-dependent mechanisms. Based on alterations in proteomes of Tam5R cell lines, we focused on iron regulation of proteins that are deregulated in tamoxifen resistance - assessing their regulation on transcriptional (mRNA) and post-transcriptional level (protein) as well as comparing their responsiveness to their sensitive parental cell line. We assessed two main types of regulation - iron-responsive element interaction with iron-regulatory proteins (IRE-IRP pathway) and tristetraprolin (TTP) driven mRNA degradation via AU-rich elements (ARE). Using iron loading and chelation, we challenged the cells - confirming the known IRE-IRP regulation of ferritin heavy chain (FTH), transferrin receptor 1 (TfR1),...
|
|
|
The role of nutrition and physical activity in breast cancer patients
Kafková, Tereza ; Matoulek, Martin (advisor) ; Tesařová, Petra (referee)
Introduction: In most countries in the world, breast cancer is the most frequent type of cancer diagnosed for women. A key factor that positively influences the prognosis of patients is physical activity, ideally supported by nutritional intervention. Adequate physical activity has a beneficial effect on increasing overall fitness and prolonging life of patients in the metastasis stage, especially in the subgroup of HER2+ patients. Furthermore, physical activity also improves the mental condition of patients. Aim: The aim of this research is to describe the effect of nutritional and physical exercise intervention for generalized breast cancer patients. The effectiveness of the interventions evaluated was based on the data from body composition measurements and the physical fitness test that was performed for the patients. Methodology: Data for this research was collected as a part of the ONKO-FIT program, which was created in co-operation with the Oncology Clinic of the General Teaching Hospital in Prague and the 1st Faculty of Medicine. The duration of this program was set for 3 months. The program was executed by 24 patients. Data was completely collected and evaluated for 13 of those patients. The patient's age range was from 39 to 57 years, with an average of 47 ± 6 years and the time since...
|
|
|
Non-pharmacological treatment of breast cancer patients
Štěpánková, Kateřina ; Matoulek, Martin (advisor) ; Hovorková, Markéta (referee)
Introduction: According to epidemiological findings,breast cancer is the second most common cancer in women. The typical age of Czech breast cancer patients should be 60-74 years, but it is estimated that up to 35 % of them are younger than 60 years. Rehabilitation, dietary interventions to prevent obesity and psychosocial care are an important part of follow-up care for these patients. The aim of this follow-up care is to improve mental and physical fitness, to facilitate the return to normal life, to prolong survival, to prevent recurrence and to treat various complications associated with both the aggressive treatment and the tumour itself. Aim: Description of changes in nutrition, physical activity and body weight in breast cancer patients who participated in the ONKOFIT - SPA programme. Methodologyand characteristics of the group: ONKOFIT - SPA is a programme that lasts for six months with the aim to determine the effect of the nutritional and physical intervention after this period. Women who were diagnosed with breast cancer were enrolled in the program. It was necessary to include data for patients who have completed 3 months of the program. Currently, 38 patients have started the program. Patients who have not yet been in the program for 3 months number 11 and are continuing the...
|
|
| |
|
|
Diabetes mellitus and breast cancer screening in Czechia in the context of socio-economic factors
Vrchlavská, Eliška ; Kulhánová, Ivana (advisor) ; Brož, Jan (referee)
This thesis focuses on the relationship of diabetes mellitus and participation in mammography screening in women in the Czechia under the influence of socioeconomic status and lifestyle factors, and it is based on the assumption that women with diabetes mellitus participate in secondary prevention of breast cancer less frequently than women without diabetes. Logistic regression analysis methods were used to assess the relationship between participation in mammography screening and diabetes, age, SES (education, household income) and lifestyle (obesity, smoking, alcohol) using European Health Interview Survey (EHIS) data from 2019. The link between SES and lifestyle is assessed for women with diabetes, as well as for women without the diagnosis. The results of this study do not show a statistically significant relationship between diabetes and participation in mammography screening, however, do demonstrate a socioeconomic gradient in participation in screening, both in the diabetes-free and diabetic populations. At the same time, they demonstrate an increased probability of participating in screening for occasional alcohol consumers. Key words: diabetes mellitus, breast cancer, mammography, EHIS, socioeconomic status
|
|
|
Physiotherapy with dynamic neuromuscular stabilization elements in oncological patients after mastectomy
Háblová, Karolina ; Aujezdská, Eva (advisor) ; Plevová, Kristýna (referee)
The bachelor's thesis deals with physiotherapy with elements of dynamic neuromuscular stabilization in oncological patients after breast ablation. The aim of the practical part is to use objective measurements to evaluate the benefit of preoperative and early postoperative physiotherapy on the patient's overall recovery. The evaluation took place three times - as a part of preoperative treatment, after surgery and after the end of the physiotherapy intervention. For this, postural locomotion tests (diaphragm test, intra-abdominal pressure test, quadruped rock forward test and test of a 3-month-old supine position), measurements of inspiratory and expiratory chest circumferences, range of motion measurements on the upper extremities and cervical spine, and measurements of arm circumferences were used. It was found that physiotherapy had a positive effect on the overall recovery of the patients. In addition, the elements of the DNS have been shown to be a suitable adjunct to the analytic exercise and could be used along with other proven methods in post-mastectomy women-patients. Keywords: Dynamic neuromuscular stabilization, breast cancer, muscle dysbalance, physiotherapy, mastectomy
|
|
|
Breast cancer - women's awareness of secondary prevention options
Košáková, Dominika ; Černý, Andrej (advisor) ; Laštůvka, Zdeněk (referee)
The topic of this bachelor thesis is breast cancer, which is one of the most common cancers in women from the Czech Republic. This thesis includes theoretical and practical part. The theoretical part was focused on the description of the issues related to breast cancer. Initially, it guides the reader through the anatomy and development of the female breast. The following chapters then introduce the reader to the different types of breast disease, either benign or malignant. Risk factors, symptoms of the disease, and current methods of diagnosis and treatment of breast cancer are also described. The last chapter elaborates on primary, secondary and tertiary prevention of breast cancer. In the practical part, two main objectives have been set. The first was to find out what is the awareness of women about secondary prevention and the second was to find out whether women are actively involved in it. These two objectives were further explored through nine sub-objectives. The research investigation was carried out using an anonymous, quantitative questionnaire survey conducted at the Gynaecology and Obstetrics Clinic of the First Faculty of Medicine of the Charles University in Prague. Data collection at the clinic took place from 1 November 2022 to 31 March 2023 and was carried out using a...
|
guest ::
