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Sexuální projevy u kanců a prasnic
JELÍNKOVÁ, Lenka
The bachelor thesis deals with sexual manifestations in boars and sows. The aim of this thesis is to monitor and evaluate sexual manifestations in boars and sows in conventional breeding. The bachelor thesis is divided into a theoretical and a practical part. The theoretical part is focused on the importance of breeding, breeding history, pig numbers and the breeding system in the Czech Republic. The focus is then placed on the development of pig genitals, sexual activity management, sexual behavior and sexual reflexes. The practical part covers the principal assessment, which is based on permanent monitoring of pigs during sexual intercourse. Individual sexual reflexes time duration, specific manifestations or caregiver interventions are closely followed. Sexual behavior was monitored in a pig herd numbering 17 boars and 71 sows (of which 10 were gilts) with a total number of 202 matings, of which 102 were successful (need for assistance being 51.8 %) and 100 unsuccessful. The results were formulated in tables and graphs.
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Vlivy působící na kvalitativní a kvantitativní znaky spermatu kanců
KUČERA, Josef
The aim of the thesis was to evaluate the effects of boar´s genotype (breed or breed combination), season, age and artificial insemination centre on sperm volume, sperm concentration, motility percentage, percentage of abnormal spermatozoa and total number of spermatozoa per ejaculate. The data were collected in 2017 and 2018 from 17 794 collections from 504 boars, 6 to 101 month of age, 6 breeds and 5 breed combinations. The boars were bred in 8 artificial insemination centres in the Czech Republic. Each boar´s genotype produced average sperm volume, sperm volume grew up from spring to autumn and to 4 years of age. Sperm concentration was lower. There were a difference 27 ×103/mm3 (P<0.01) between spring and winter. Boars between 2 to3 years of age had higher sperm concentration (14 ×103/mm3 resp. 15.2 ×103/mm3) than boars less 1 year, resp. 2 to 3 years of age (P<0.01). Sperm motility was at good quality in all genotypes and artificial insemination centres, in all seasons and every age group. Total number of spermatozoa per ejaculate was lower for most genotypes, grew to 4 years of age, there were statistically significant differences between spring and autumn 9 bill.
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Isolation and study of proteins with zona pellucida binding activity
Zigo, Michal ; Postlerová, Pavla (advisor) ; Ryšlavá, Helena (referee) ; Antalíková, Jana (referee)
Binding of sperm to the oocyte is mediated by complementary molecules on the surface of both gametes and involves the interaction of sperm protein receptors with the zona pellucida (ZP) saccharide structures. It has been shown that many proteins receptors are involved in the sperm-ZP interaction, and potential primary sperm receptors for ZP glycoproteins have been investigated in various mammals. The majority of proteins with identified sperm-ZP binding activity belong to the plasma membrane proteins. However, the exact methods for isolation of sperm membrane proteins are still to be standardized. This study is focused on investigating how employment of various isolation protocols leads to acquisition of various protein mixtures. Further in the work, two possible approaches towards identification of potential ZP-binding partners are implemented, in order to disclose novel primary ZP-binding receptor candidates. Sperm proteins of ejaculated and in vitro capacitated boar sperms were isolated by: Triton X-100, Triton X-114, acetic acid, sodium dodecyl sulphate (SDS), N-octyl-β-D- glucopyranoside (OBG), rehydration buffer (RHB), and finally by freezing-thawing extraction and they were characterized by 1-D, 2-D protein profiles, glycoprotein staining and substrate zymographic methods. The results have...
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Species-specific structural differences of mammalian sperm and function of their key proteins during fertilization.
Dobrodinská, Anna ; Frolíková, Michaela (advisor) ; Kuntová, Barbora (referee)
The fertilization is a process during which a male and a female gamete merge so that a new organism may come into being. The sperm-egg fusion is preceded by several essential processes, such as the capacitation, acrosome reaction, the sperm binding to the zona pellucida and oolemma, and membrane fusion of the gametes. Numerous proteins, which are located in both sperm and eggs, are major actors in controlling the listed, essential processes. During the process of fertilization these proteins fulfil one or more functions. In mammalian sperm, significant species-specific differences may be found both in their morphology and at the protein level. A complex understanding of species-specific distinctions in sperm structure and functions of key sperm proteins would contribute to a better insight into the process of fertilization, thereby enabling us to better diagnose and subsequently treat the causes of infertility in humans. This bachelor's thesis summarizes the current knowledge of sperm structure and its key proteins that has been acquired through the studies of the following model mammal species: bull, boar, mouse, and human. Further, this thesis brings an interspecific comparison between the studied species. Keywords: sperm, fertilization, acrosome reaction, capacitation, sperm proteins, bull,...
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Boar vocalizations and their potential role in sexual selection
Roreitnerová, Daniela ; Linhart, Pavel (advisor) ; Schneiderová, Irena (referee)
The aim of this research was to gather existing knowledge about vocalizations of boars and their potential role in sexual selection. Few studies mainly from the 70s and 80s of the last century suggest that the so-called love song, emitted by males during courtship, could act as a stimulant that triggers mating. However, I did not find any evidence that boars significantly use calls in fights. Most studies deal with pig vocalization in a different context. For this reason, I tried to look at the studies of pig relatives - even-toed ungulates - to better evaluate the potential role of boar vocalizations in sexual selection. In their case, the influence of the calls on the sexual behavior of females and competing males was reported. It is possible to conclude that the role of boar vocalization is probably not that important as in red deers, for example, because boars use calls in short range communication. On the other hand, stimulatory effect on females was found in other species too. Further studies are needed to understand possible function of boar vocalizations that could be used for example in pig breeding to improve the effectiveness of artificial insemination of sows. Powered by TCPDF (www.tcpdf.org)
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Isolation and study of proteins with zona pellucida binding activity
Zigo, Michal
Binding of sperm to the oocyte is mediated by complementary molecules on the surface of both gametes and involves the interaction of sperm protein receptors with the zona pellucida (ZP) saccharide structures. It has been shown that many proteins receptors are involved in the sperm-ZP interaction, and potential primary sperm receptors for ZP glycoproteins have been investigated in various mammals. The majority of proteins with identified sperm-ZP binding activity belong to the plasma membrane proteins. However, the exact methods for isolation of sperm membrane proteins are still to be standardized. This study is focused on investigating how employment of various isolation protocols leads to acquisition of various protein mixtures. Further in the work, two possible approaches towards identification of potential ZP-binding partners are implemented, in order to disclose novel primary ZP-binding receptor candidates. Sperm proteins of ejaculated and in vitro capacitated boar sperms were isolated by: Triton X-100, Triton X-114, acetic acid, sodium dodecyl sulphate (SDS), N-octyl-β-D- glucopyranoside (OBG), rehydration buffer (RHB), and finally by freezing-thawing extraction and they were characterized by 1-D, 2-D protein profiles, glycoprotein staining and substrate zymographic methods. The results have...
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Species-specific structural differences of mammalian sperm and function of their key proteins during fertilization.
Dobrodinská, Anna ; Frolíková, Michaela (advisor) ; Kuntová, Barbora (referee)
The fertilization is a process during which a male and a female gamete merge so that a new organism may come into being. The sperm-egg fusion is preceded by several essential processes, such as the capacitation, acrosome reaction, the sperm binding to the zona pellucida and oolemma, and membrane fusion of the gametes. Numerous proteins, which are located in both sperm and eggs, are major actors in controlling the listed, essential processes. During the process of fertilization these proteins fulfil one or more functions. In mammalian sperm, significant species-specific differences may be found both in their morphology and at the protein level. A complex understanding of species-specific distinctions in sperm structure and functions of key sperm proteins would contribute to a better insight into the process of fertilization, thereby enabling us to better diagnose and subsequently treat the causes of infertility in humans. This bachelor's thesis summarizes the current knowledge of sperm structure and its key proteins that has been acquired through the studies of the following model mammal species: bull, boar, mouse, and human. Further, this thesis brings an interspecific comparison between the studied species. Keywords: sperm, fertilization, acrosome reaction, capacitation, sperm proteins, bull,...
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Analýza morfologických změn spermií kanců a jejich vliv na plodnost prasnic
ŠTVERÁK, Martin
The aim of the work was to evaluate the quality of boar ejaculate in terms of sperm morphology and the influence on fertility of sows. The data came from 58 sperm collections from 8 boars of one line. The boars were housed in the semen collection centre under the same conditions and were in age from 11 to 21 months. In the ejaculate analysis, the evaluation of the pathologically changed sperms was performed by determining the frequency of the finding of individual morphological changes. To evaluate the effect of semen on the litter size, data from 123 successful inseminations and subsequent births were processed. The results showed that boars in most cases produced ejaculate with an average volume with a lower sperm concentration. The incidence of morphologically abnormal sperms was normal for almost all boars. The most common sperm abnormalities were immature sperms and defects of sperm flagella. It was confirmed that more piglets were born after using insemination doses made from sperm ejaculate with the higher sperm count. Furthermore, when using ejaculate with a morphologically abnormal sperm count below 15%, more piglets were born than when the sperm count was 1525%. In the case of immature sperms, a negative correlation with the number of born piglets has been proven.
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Boar vocalizations and their potential role in sexual selection
Roreitnerová, Daniela ; Linhart, Pavel (advisor) ; Schneiderová, Irena (referee)
The aim of this research was to gather existing knowledge about vocalizations of boars and their potential role in sexual selection. Few studies mainly from the 70s and 80s of the last century suggest that the so-called love song, emitted by males during courtship, could act as a stimulant that triggers mating. However, I did not find any evidence that boars significantly use calls in fights. Most studies deal with pig vocalization in a different context. For this reason, I tried to look at the studies of pig relatives - even-toed ungulates - to better evaluate the potential role of boar vocalizations in sexual selection. In their case, the influence of the calls on the sexual behavior of females and competing males was reported. It is possible to conclude that the role of boar vocalization is probably not that important as in red deers, for example, because boars use calls in short range communication. On the other hand, stimulatory effect on females was found in other species too. Further studies are needed to understand possible function of boar vocalizations that could be used for example in pig breeding to improve the effectiveness of artificial insemination of sows. Powered by TCPDF (www.tcpdf.org)
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Isolation and study of proteins with zona pellucida binding activity
Zigo, Michal ; Postlerová, Pavla (advisor) ; Ryšlavá, Helena (referee) ; Antalíková, Jana (referee)
Binding of sperm to the oocyte is mediated by complementary molecules on the surface of both gametes and involves the interaction of sperm protein receptors with the zona pellucida (ZP) saccharide structures. It has been shown that many proteins receptors are involved in the sperm-ZP interaction, and potential primary sperm receptors for ZP glycoproteins have been investigated in various mammals. The majority of proteins with identified sperm-ZP binding activity belong to the plasma membrane proteins. However, the exact methods for isolation of sperm membrane proteins are still to be standardized. This study is focused on investigating how employment of various isolation protocols leads to acquisition of various protein mixtures. Further in the work, two possible approaches towards identification of potential ZP-binding partners are implemented, in order to disclose novel primary ZP-binding receptor candidates. Sperm proteins of ejaculated and in vitro capacitated boar sperms were isolated by: Triton X-100, Triton X-114, acetic acid, sodium dodecyl sulphate (SDS), N-octyl-β-D- glucopyranoside (OBG), rehydration buffer (RHB), and finally by freezing-thawing extraction and they were characterized by 1-D, 2-D protein profiles, glycoprotein staining and substrate zymographic methods. The results have...
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