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The characterization of Pseudomonas syringae biofilm and its interaction with antibacterial agents - biophysical approaches
Budil, Jakub ; Lišková, Petra (advisor) ; Mackuľak, Tomáš (referee) ; Viktorová, Jitka (referee)
4 Abstract The objectives of this thesis are the application of Attenuated Total Reflectance Fourier Transform Infrared (ATR FT-IR) spectroscopy for the analysis of Pseudomonas syringae biofilms grown in a flow cell and its subsequent interaction with antibacterial agents. Based on the combination of the recorded ATR spectra with the biofilm morphology analysis via light and fluorescent microscopy, I suggested a model for the development of Pseudomonas syringae pathovar morsprunorum CCM 2534 biofilm in a flow cell with 2% Luria-Bertani (LB) complex medium. This model consists of the initial colonization of the ZnSe prism (adhesion), subsequent washing of weakly attached cells due to the replacement of the inoculation suspension with the fresh 2% LB medium, which is followed by a brief substrate recolonization phase. Subsequently, the biofilm undergoes a restructuration with a motile subpopulation of Pseudomonas syringae migrating on top of already existing early microcolonies, where the availability of nutrients including oxygen is higher. The last developmental stage, a maturation of Pseudomonas syringae biofilms, is occurring predominantly outside the ATR FT-IR detection range and leads to a formation of large mushroom-shaped microcolonies. I also investigated the changes to the development of Pseudomonas...
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Antibakteriální povrchové úpravy nábytkových dílců
Frkalová, Veronika
This thesis deals with the determination and evaluation of antibacterial properties of coatings and surface finishing. The thesis verified antibacterial, physico-mechanical and chemical properties of water-based coatings themselves with added antibacterial particles. For better orientation in issue of this thesis are generally described coatings, antibacterial substances and tested bacteria. The aim is to determine the effect on the properties of coatings with added antibacterial substances. How effective is the resistance of the tested coatings with added ingredients to the action of bacteria and fungi as well as to assess whether they are suitable for use in surface finishing furniture parts and whether they have the expected properties.
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