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Pet-Keeping and its Importance
Šlajchrtová, Kristýna ; Hanel, Lubomír (advisor) ; Andreska, Jan (referee)
Title: Pet-Keeping and its Importance Autor: Bc. Kristýna Šlajchrtová Supervisor: Prof.RNDr. Lubomír Hanel, CSc. The thesis deals with the issue of the importance of domesticated animal breeding in households focusing in Central Bohemia and Prague. The theoretical part discussed the issue of domesticated animal breeding in our country and abroad, history of breeding, venomous and dangerous species breeding and breeding of endangered species. The thesis also describes issues of animal shelters, the negatives of transmittable diseases to humans and the positives of human contact with nature (canistherapy, felistherapy). That research was collected via anonymous questionnaires. In the research part the obtained conclusions were evaluated by questionnaire survey. The aim was to determine which domestic animals are the most popular and most frequently breeded in certain types of families (children's influence on breeding, comparison of housing and animal husbandry in the town and village). Keywords Pets, home, family, livestock, standardized questionnaires, zootherapy
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Validation of PCR-RFLP method for determination of lactose intolerance
PROSOVÁ, Denisa
The lactose intolerance is the most common food intolerance occurring worldwide. Individuals having the lactose intolerance cannot produce the laktase enzyme in thein small intestine. This enzyme is able to digest the lactose contained in milk. There are several wals for this disabilitty. The practical part of this bachaleor thesis is focused on the genetic origin of the lactose intolerance. In the population of Europe we can find as the most frequent the two polymorphism of the MCM6 gene that affect the production of laktase C/T13910 and G/A22018. In principle it concerns the new gene station which ensure the production of laktase enzyme even in adult age. The aim of this bachaleor thesis is to creat the research based on the recent knowledge Publisher in the specialized literature. There are gin to be describe all type sof the lactose intolerance and the ways of its diagnostics, futhermore e.g. the princupli of Alleny, the used laboratoř methods, the rule sof validation methods etc.. The pros and cons of every testing approach will be explained. The practical part is aiming at the implementation of the validation of the genetics test used for petting down the lactose intolerance and creating the validation protokol that will serve as a base of my bachaleor thesis. The genetic Cheb-up is based on the PCR-RFLP principle, within the metodology the laboratoř procedures being used in the genetic lab will be described. The validaton will flow into a detection if the applied method is sufficiently valid fot the diagnostics. The genetic analysis will focus on the two selected polymorphism C/T13910 and G/A22018. The sample with a familiar genotype that has already been verifie by sekvenation will be used for validation.
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Pet-Keeping and its Importance
Šlajchrtová, Kristýna ; Hanel, Lubomír (advisor) ; Andreska, Jan (referee)
Title: Pet-Keeping and its Importance Autor: Bc. Kristýna Šlajchrtová Supervisor: Prof.RNDr. Lubomír Hanel, CSc. The thesis deals with the issue of the importance of domesticated animal breeding in households focusing in Central Bohemia and Prague. The theoretical part discussed the issue of domesticated animal breeding in our country and abroad, history of breeding, venomous and dangerous species breeding and breeding of endangered species. The thesis also describes issues of animal shelters, the negatives of transmittable diseases to humans and the positives of human contact with nature (canistherapy, felistherapy). That research was collected via anonymous questionnaires. In the research part the obtained conclusions were evaluated by questionnaire survey. The aim was to determine which domestic animals are the most popular and most frequently breeded in certain types of families (children's influence on breeding, comparison of housing and animal husbandry in the town and village). Keywords Pets, home, family, livestock, standardized questionnaires, zootherapy
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