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Procalcitonin as an early marker of bacterial infection
Mikulová, Lenka ; Beránek, Martin (advisor) ; Drastíková, Monika (referee)
Introduction: Procalcitonin (PCT) is a 13 kD protein with a chain of 116 amino acids. It consists of parafollicularcells of the thyroid gland. Inflammatory procalcitonin is made up of 114 amino acids. Procalcitonin from plasma in the course of inflammatory process, does not consistof C-cells of the thyroid gland but it is generatedin the liver, and, to a lesser extent, in the lungs, kidneys, testes, prostate and small intestine. Objectives: 1. To compare the levels and the dynamics of C-reactive protein (CRP) and procalcitonin in the inflammatory process. in three patients with different diagnoses. To compare the number of examined PCT according to indicated VFN (General University Hospital) departments in the years 2009, 2012 and 2015. 3. To study the dynamics of the number of examined PCT in the years 2001- 2016. Methods: The levels of CRP and PCT were measured in the ÚLBLD laboratory of the General University Hospital in Prague in the period from May 20to June 17, 2016. The CRP was measured by immunoturbidimetricanalyzerUnicelDxC 880i. The PCT was measured by theCobas analyser using the electrochemiluminescence technology. In order to evaluate the data, graphs were processed with levels of bothanalytesfor 3 patients. Further, data needed for the evaluation of the above mentioned objectives no. 2...
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Procalcitonin as an early marker of bacterial infection
Mikulová, Lenka ; Beránek, Martin (advisor) ; Drastíková, Monika (referee)
Introduction: Procalcitonin (PCT) is a 13 kD protein with a chain of 116 amino acids. It consists of parafollicularcells of the thyroid gland. Inflammatory procalcitonin is made up of 114 amino acids. Procalcitonin from plasma in the course of inflammatory process, does not consistof C-cells of the thyroid gland but it is generatedin the liver, and, to a lesser extent, in the lungs, kidneys, testes, prostate and small intestine. Objectives: 1. To compare the levels and the dynamics of C-reactive protein (CRP) and procalcitonin in the inflammatory process. in three patients with different diagnoses. To compare the number of examined PCT according to indicated VFN (General University Hospital) departments in the years 2009, 2012 and 2015. 3. To study the dynamics of the number of examined PCT in the years 2001- 2016. Methods: The levels of CRP and PCT were measured in the ÚLBLD laboratory of the General University Hospital in Prague in the period from May 20to June 17, 2016. The CRP was measured by immunoturbidimetricanalyzerUnicelDxC 880i. The PCT was measured by theCobas analyser using the electrochemiluminescence technology. In order to evaluate the data, graphs were processed with levels of bothanalytesfor 3 patients. Further, data needed for the evaluation of the above mentioned objectives no. 2...
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Gene expression profiling in diabetic nephropathy
Nepomucká, Kateřina ; Pavlínková, Gabriela (advisor) ; Černá, Věra (referee)
Diabetic nephropathy (DN) is induced by both type 1 and type 2 diabetes mellitus and it is one of the most serious complications associated with diabetes. Despite increasing incidence of diabetes, the exact pathogenesis remains unclear. Hypoxia is regarded as a crucial factor for the progression of renal disease. The responses to hypoxia are mainly regulated by hypoxia-inducible factor 1 (HIF1). We thus considered a possible link between HIF1-regulated pathways and the susceptibility to DN and the disease progression. We hypothesize that the exposure of renal tissue to diabetes causes gene expression changes in HIF1-regulated pathway and the altered expression profile is decisive for the development of DN. Using mouse model, we analyzed cellular and molecular changes in HIF1 heterozygous-null (Hif1α+/- ) and wild type (wt) littermates exposed to diabetic environment. Our histological analysis showed early pathological changes associated with DN in both diabetic wt and Hif1α+/- compared to non-diabetic controls. The morphological analysis did not demonstrate the effect of Hif1α+/- genotype in comparison to wt. For our molecular analysis with qRT-PCR method, we selected several genes, which were previously associated with pathological processes in kidney diseases. We identified statistically...
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