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Validity of the VO2 Master Pro device for maximal oxygen consumption prediction and the measurement of resting metabolism
Goldschmidt, Tobiáš ; Nohel, Michal (referee) ; Chlíbková, Daniela (advisor)
Measurements of maximal oxygen uptake and resting metabolic rate are one of the most important things in testing athletes and madical patients. For these measurements are used breath analyzers. These analyzers are usually non-portable costly devices. VO2 Master Pro is on the other hand portable breath analyzer with much lower prize. It is biggest disadvantage is that it contains only O2 sensor and not CO2 sensor as madically aproved devices. Aim of this study is to determinate validity and difference of measurements between VO2 Master Pro and reference device for measuring maximal oxygen uptake and resting metabolic rate. Difference for measuring maximal oxygen uptake was 4,15 ± 4,41 %. For measuring of resting metabolic rate difference was 41,20 ± 11,44 %. For measuring of maximal oxygen uptake is VO2 Master Pro valid but for measuring of resting metabolic rate it is not. It was also confirmed, that size of measured value have non-linear corelation with size of difference in measuring maximal oxygen uptake.
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Validity of the VO2 Master Pro device for maximal oxygen consumption prediction and the measurement of resting metabolism
Goldschmidt, Tobiáš ; Nohel, Michal (referee) ; Chlíbková, Daniela (advisor)
Measurements of maximal oxygen uptake and resting metabolic rate are one of the most important things in testing athletes and madical patients. For these measurements are used breath analyzers. These analyzers are usually non-portable costly devices. VO2 Master Pro is on the other hand portable breath analyzer with much lower prize. It is biggest disadvantage is that it contains only O2 sensor and not CO2 sensor as madically aproved devices. Aim of this study is to determinate validity and difference of measurements between VO2 Master Pro and reference device for measuring maximal oxygen uptake and resting metabolic rate. Difference for measuring maximal oxygen uptake was 4,15 ± 4,41 %. For measuring of resting metabolic rate difference was 41,20 ± 11,44 %. For measuring of maximal oxygen uptake is VO2 Master Pro valid but for measuring of resting metabolic rate it is not. It was also confirmed, that size of measured value have non-linear corelation with size of difference in measuring maximal oxygen uptake.
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Effect of body composition on resting metabolic rate in obese patients
Staňová, Alžběta ; Sadílková, Aneta (advisor) ; Mikeš, Ondřej (referee)
Introduction: Changes in eating habits and incorporation of physical activities remain to be the main methods in treating obesity. To set an adequate calorie intake, which would lead to weight loss, it is necessary to determine energy needs of an individual. For that energy intake and energy expenditure need to be determinate. However, energy expenditure is difficult to determine since it is contributed of three components. Basal, respectively resting metabolic rate (RMR), which is affected by a body composition, has the greatest influence on a total energy expenditure. Indirect calorimetry (IC) is considered to be a gold standard to determine RMR. When RMR is calculated by prediction equations instead, such as Harris-Benedict (H-B) equation, energy needs are overestimate or underestimate for people with abnormal body composition. Objectives: The aim of this study is to analyse data of RMR obtained using IC in connection to body composition. The main aim is to determine how fat free mas (FFM) and fat mass (FM) are affecting RMR in obese patients. There is also a comparation of RMR measured by the IC and RMR calculated using H-B equation as a part of this study, while IC is considered to be a reference method. Methods: Patients new to ambulance of obesitology at General University Hospital in Prague...
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Use of indirect calorimetry in nutritional therapy
Pokorná, Veronika ; Sadílková, Aneta (advisor) ; Žůrková, Pavla (referee)
Introduction: Indirect calorimetry (IC) is a very accurate and non-invasive method which is used in clinical practice to measure the resting metabolic rate. This method is based on the assumption that oxygen consumption, carbon dioxide production and nitrogen metabolite waste are in definite relation to energy consumption. Based on the measurement of resting metabolic rate, a nutritional therapist can individually determine daily energy intake of a respective individual, the optimal amount of specific nutrients and thus prevents excessive or inadequate intake of energy and nutrients. Objective: The main aim of this thesis is to compare for group of overweight patients the results of the measurement of the resting metabolic rate (RMR), values of the resting metabolic rate calculated according to the Harris-Benedict (HB) equation and the actual energy intake calculated from the diet report obtained from the patients. In addition, the body composition of the examined group with a focus on the amount of muscle tissue is also marginally evaluated. Methods: The research sample includes in total 50 persons-36 women and 14 men. These persons are patients of the General Faculty Hospital in Prague. The average age is 46 years for women and 51 years for men. The average BMI for women is 38,2 kg/m2 and for men...
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Mammalian energetic savings in subterranean environment. The case of African mole-rats.
OKROUHLÍK, Jan
Mole-rats are placental mammals which are perfectly adapted to subterranean life. In this thesis I present novel findings on working metabolism and thermoregulatory physiology of mole-rats. These animals cope with low availability of food and have thus employed multiple strategies how to conserve energy and/or use it more effectively. Among other adaptations this resulted in lower resting body temperature, tolerance to increase in body temperature during exercise or while at rest, surprisingly efficient cooling while digging and precise diurnal and seasonal timing of activity with regards to environmental conditions. My focus in this work is on the digging metabolic rate and thermoregulation of social Fukomys mechowii and solitary Heliophobius argenteocinereus in soft and hard substrate, thermoregulatory abilities of Fukomys darlingi, seasonal changes of activity in free living Heliophobius argenteocinereus measured as daily energy expenditure and, finally, energetic consequences of the daily activity patterns of Fukomys anselli.
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Poikilothermic traits in Mashona mole-rat (Fukomys darlingi). Reality or myth?
ZEMANOVÁ, Milada
The African mole-rats is a mammalian family well known for a variety of ecophysiological adaptations for strictly belowground life. The Mashona mole-rat (Fukomys darlingi) is supposed to have strong poikilothermic traits, because it is not able to maintain a stable body temperature at ambient temperatures below 20°C. In our study, we measured resting metabolic rate and body temperature of Mashona mole-rats across a gradient of ambient temperatures to test its poikilothermic traits. Tested mole-rats show typical mammalian pattern in resting metabolic rates. And their body temperature was stable at ambient temperatures from 10 to 25°C. We thus cannot confirm poikilothermic traits in this species.
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Bioenergetics of reproduction and postnatal development of two species of social mole-rat of genus \kur{Fukomys}
ZEMANOVÁ, Milada
The resting metabolic rate of mole-rats was measured with a close-flow respirometry system and body temperature was recorded with a rectal thermometer at an ambient temperature within and below adult thermoneutrality range. The development of ability to thermoregulate was monitored for pups of Fukomys sp. Huddling and presence of adults reduced the body heat loss of pups of all monitored age, but no effect was observed on the metabolic rate of the pups until they were two months old. Huddling had no effect on resting metabolic rate and body temperature in adult giant mole-rats Fukomys mechowii. The maternal cost of reproduction was evaluated in females from two species of the genus Fukomys. The energetic expenditure of females increased with pup´s age.
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The Thermoregulatory Abilities in a Mole-rat \kur{Fukomys darlingi} and its Development in Pups
ZEMANOVÁ, Milada
The oxygen consumption and body temperature were measured in adults and pups of a social Mashona mole-rat(Fukomys darlingi)to test poikilothermic traits in this species and effect of presence of adults on pup´s thermoregulation abilities. The adult´s resting metabolic rate was 0.76 {$\pm$} 0.20 mlO2g-1hod-1 in the thermoneutral zone 27-34°C. We did not confirm poikilotermic traits in this species, because body temperature was stable (33.0 {$\pm$} 0.5°C) at low ambient temperatures. The pups started to thermoregulate in age of one month and they are able to maintain stable body temperature very late in age of three months. My results indicate that presence of adults is necessary for thermoregulation of pups.
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