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The role of Whi3p in mitosis in Saccharomyces cerevisiae
Lazarová, Monika ; Schierová, Michaela (advisor) ; Zikánová, Blanka (referee)
RNA binding protein Whi3p in the yeast cell affects a variety of processes, the most important is the regulation of the yeast cell size when entering the S phase. Recently, a link between Whi3p protein and yeast cell ploidy has been discovered. It has been shown that in strains lacking the WHI3 gene (whi3Δ) there is an increase in ploidy. The Whi3p protein affects the expression of genes coding cohesin subunits and also regulates an amount of Nip100p on the post-transcriptional level. Nip100p is an important part of the dynactin complex. Both possibilities of regulation are closely related to the correct chromosome distribution during anaphase of the mitotic division. Microarray and immunoprecipitation analyzes demonstrate Whi3p interaction with more than 400 mRNAs. By translating these target mRNAs both structural and regulatory proteins, that are essential for the standard course of nuclear division, are formed. The specific consequences of Whi3p interactions with target RNAs are not known, however, they point to the unknown role of Whi3p protein in the mitotic division of budding yeast. Key words: Whi3p, RNA binding protein, dynactin complex, regulatory principles in anaphase, Saccharomyces cerevisiae
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