Original title: Post-transcriptional regulation of TbIF1 in life cycle of Trypanosoma brucei
Authors: GRATZL, Sascha
Document type: Bachelor's theses
Year: 2020
Language: eng
Abstract: TbIF1, a protein Inhibitor of F1-ATPase in Trypanosoma brucei, is expressed exclusively in the insect stage of the parasite. In the bloodstream form, TbIF1 is switched off, because its activity interferes with the essential role of the ATP synthase in the maintenance of the mitochondrial membrane potential. Here, we employ a series of reporter genes to study the impact of 3'UTR of TbIF1 on mRNA stability and translatability to get insight into the tight post-transcriptional control of TbIF1. We provide evidence that developmentally regulated RNA binding protein Rbp10 is critical for downregulation of TbIF1 on translation level in bloodstream-form trypanosomes.
Keywords: ATP synthase; Chloramphenicol acetyltransferase; ELISA; post-transcriptional regulation; Rbp10; Rbp6; reporter system; ribosmome profiling; RNA binding protein; RT-qPCR; translation efficiency; Trypanosoma brucei
Citation: GRATZL, Sascha. Post-transcriptional regulation of TbIF1 in life cycle of Trypanosoma brucei. České Budějovice, 2020. bakalářská práce (Bc.). JIHOČESKÁ UNIVERZITA V ČESKÝCH BUDĚJOVICÍCH. Přírodovědecká fakulta

Institution: University of South Bohemia in České Budějovice (web)
Document availability information: Fulltext is available in the Digital Repository of University of South Bohemia.
Original record: http://www.jcu.cz/vskp/56015

Permalink: http://www.nusl.cz/ntk/nusl-514132


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Universities and colleges > Public universities > University of South Bohemia in České Budějovice
Academic theses (ETDs) > Bachelor's theses