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Microplastics in soil
Dvořáková, Alena ; Kučerík, Jiří (referee) ; Doležalová Weissmannová, Helena (advisor)
This bachelor thesis deals with the appraisal of the suitability of infrared spectroscopy for the identification of plastics and microplastics in soils. Available techniques were DRIFT (diffuse reflectance) and ATR (attenuated total reflectance). Mixtures of model soil and pure PET were prepared for analysis in various concentrations. Furthermore, a total of 21 samples of soil and 3 samples of compost were evaluated. The obtained data were used for interpretation of infrared spectra, creation of calibrations of PET using ATR and DRIFT methods. Part of the work was the evaluation of validation models using spectra and basic soil parameters.
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The development of a TGA-MS based method for determination of microplastics in soils
Šilhánková, Lenka ; Mravcová, Ludmila (referee) ; Kučerík, Jiří (advisor)
Plasty, pronikající do životního prostředí ať už neúmyslně či záměrně, mohou být fragmentovány za vzniku částic v řádu mikrometrů (tzv. mikroplastů) a znečišťovat tak půdní systémy. Doposud vyvinuté analýzy pro stanovení mikroplastů v půdách jsou především zaměřeny na kvalitativní stanovení, nicméně i jejich provedení se zatím stále potýká s různými úskalími. Navíc, téměř všechny tyto analýzy vyžadují časově náročnou předúpravu vzorku. V této studii jsme se zaměřili na vývoj nové analytické metody pro kvantitativní stanovení mikroplastů polyvinylchloridu (PVC) a polystyrenu (PS) v půdách bez předchozí úpravy vzorku pomocí termogravimetrické analýzy spojené s hmotnostní spektrometrií (TGA-MS). Pro analýzu byly použity vzorky modelové půdy s nízkým obsahem organického uhlíku, které byly spikovány na výslednou koncentraci 0,23–7 hm% PVC či PS. Vzorky byly pyrolyzovány s teplotním krokem 5 K min–1 až na teplotu 1000 °C. Pyrolýzní plynné produkty byly následně analyzovány s využitím hmotnostního spektrometru. Získaná data byla normalizována pomocí externího standardu (šťavelan vápenatý). Limity detekce se pohybovaly v rozmezí 0,08–5,3 hm% pro PVC a 0,005–0,7 hm% pro PS v závislosti na zvoleném m/z. Limity kvantifikace pak byly 0,3–17,7 hm% pro PVC a 0,002–2,2 hm% pro PS. Výsledky dokazují, že spojení TGA-MS může být konkurující semikvantitativní metodou pro stanovení mikro-PVC a mikro-PS v půdě s nízkým obsahem organického uhlíku.
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Elimination of nanoplastics by cavitation and advanced oxidation processes
Kluknavská, Júlia ; Burda, Radim (referee) ; Rudolf, Pavel (advisor)
Táto práca sa zaoberá znečistením životného prostredia mikro a nanoplastami a ich možnou likvidáciou. Práca pozostáva z rešeršnej a experimentálnej časti. V rámci rešerše je opísaná problematika mikro a nanoplatov z hľadiska ich vzniku, disperzie do životného prostredia a živých organizmov. V závere rešeršnej časti sú opísané súčasné možnosti eliminácie mikro a nanoplastov pri čistení odpadových vôd. Súčasné riešenia sú založené prevažne na filtrácii, ktorá však zo svojej podstaty nemôže byť absolútna. Bol vyslovený predpoklad, že pomocou zariadenia CaviPlasma by bolo možné eliminovať mikro a nanoplasty z odpadových vôd. Cieľom práce bolo experimentálne overiť tento predpoklad. Skúšobnou vzorkou bola suspenzia vody a polyamidových častíc, ktorá bola následne vystavená pôsobeniu kavitácie a plazmového výboja. Pri experimente nedošlo ku zničeniu častíc a ani ich k viditeľnému porušeniu. Ukázalo sa však, že plazmový výboj môže iniciovať zhlukovanie sa častíc až do takej miery, že sú pozorovateľné kontinuálne štruktúry mikroplastov. Tento jav by mohol byť potenciálne prospešný pri zachytávaní mikročastíc filtráciou.
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Study of the medium influence on the pollutants sorption on microplastics
Hamplová, Marie ; Kučerík, Jiří (referee) ; Mravcová, Ludmila (advisor)
With the increasing production of plastic waste, the pollution of the environment by persistent microplastics is increasing simultaneously. Other environmental pollutants, such as various pharmaceuticals, can be sorbed on microplastics. This increases the toxicity of microplastics and their risk to living organisms. Nowadays, the use of so-called biodegradable polymers, which are supposed to degrade spontaneously, is expanding. However, their degradation can also produce microplastics. Therefore, this bachelor thesis deals with the sorption of drugs onto microplastics from biodegradable polyhydroxybutyrate (PHB) and with the influence of the surrounding aqueous environment on the rate and mechanism of sorption. Sorption of drugs onto microplastics made of synthetic polyethylene terephthalate (PET) was also carried out for comparison. The quantity of drugs was analysed by UPLC-ESI-TQ. Sorption was observed from the drug mixture for antibiotics tetracyclines. The pH value of the surrounding environment influenced the sorption rate of tetracyclines, the highest sorption occurred at acidic pH 4. According to the results of evaluation of sorption by Langmuir and Freundlich adsorption isotherms, PHB microplastics have a higher sorption capacity than PET microplastics. Therefore, PHB microplastics could be a risk for living organisms.
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Plastics and microplastics in water
Halašta, Tomáš ; Kučera, Tomáš (referee) ; Biela, Renata (advisor)
This bachelor thesis deals with the occurrence of plastics and microplastics in water and also is mainly devoted to microplastic in water treatment plants. The beginning of the work is devoted to plastics, their history, description of properties and what are the most commonly used plastics, which can be found in the form of microplastics. Next topic is devoted to the occurrence of plastics in waters, mainly seas and oceans, and especially in freshwater systems. Then the definition of microplastics and their sources and occurrence is given in the work. Furthermore, the work deals with studies on the occurrence of microplastics in drinking water and their removal at water treatment plants and the analysis of plastics microparticles. The conclusion is a summary of all available studies on microplastics in drinking water and water treatment plants.
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Problems of plastics and microplastics in FTIR analysis of soils
Stanislavová, Veronika ; Kučerík, Jiří (referee) ; Doležalová Weissmannová, Helena (advisor)
Microplastics are formed as the result of the fragmentation of plastics. The microparticles are easier to distribute and thus can contaminate the environment. Recently, there has been an increasing interest in the research of microplastics present in the soil system, using different methodologies. The focus of this thesis is the analysis of microplastics in soil using infrared spectroscopy – ATR (attenuated total reflectance) and DRIFT (diffuse reflectance) techniques. This work aimed to evaluate the suitability of the method for the identification of microplastics in soils. Polymers such as polyethylene terephthalate (PET), poly-3-hydroxybutyrate (PHB) and polylactic acid (PLA) were used. In the first part of experimental analysis were microplastics mixed in different concentrations with 3 different types of model soils. The obtained infrared spectra of samples of mixtures (model soil and plastics) were used for statistical evaluation. The second part of the measurement was focused on the monitoring of changes in the spectra of real soils spiked with PET, after exposure to the degradation. After the interpretation of infrared spectra of samples of plastics with real and model soil, it was possible to confirm the presence of the functional groups of each polymer. Based on the measurements, the used techniques were evaluated as inappropriate for the qualitative determination of PLA. The results of the experimental part confirm the accuracy of FTIR (both ATR and DRIFT) for the analysis of microplastics in soil for samples containing PET and PHB.
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Microplastics in solid matrices of the environment
Pospíchalová, Eliška ; Cajthaml, Tomáš (advisor) ; Innemanová, Petra (referee)
Microplastics, plastic particles of 5 mm-1 nm in size, are now widespread across different ecosystems. The increasing amount of microplastic contamination of aquatic and terrestrial environments requires the development of standardized methods for their separation and analysis. One of the main vectors of microplastic input to terrestrial ecosystems is through sewage sludge. The sludge is recycled for fertilization and reclamation due to its high organic matter content. Scientists have already been able to quantify and identify a range of microplastics in sewage sludge of different sizes, polymer types, shapes, etc. This thesis focuses on the optimization of existing methodologies to extract microplastics from solid environmental samples and their subsequent qualitative and quantitative analysis. The steps required for sample pre-treatment prior to analysis, namely reduction in organic matter content by the Fenton reaction process and density-based separation of microplastics by flotation in saturated NaI solution (1.8 g/cm3 ) using centrifugation, were optimized. Microplastics in size fractions of 20-40 μm and 150-300 μm were fabricated and introduced into flotation and total recovery experiments of the methodology to determine the recovery. Fluorescence microscopy was chosen for quantitative...
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Mikroplasty a jejich vliv na životní prostředí
Kopicová, Jana
This bachelor thesis deals with plastic pollution in the environment predominantly focusing on microplastics. The aim of the theoretical part is to acquaint readers with the terminological background of plastics and their microscopic parts. This part also contains an overview of current information about sources, presence, and possible impact of microplastics in the environment centring on the water component. The theoretical part further discusses the effect of microplastics on living organisms and one’s health. In the practical part of the thesis, a questionnaire survey was carried out to find out respondents’ opinions and awareness of the given matter. The results of the survey confirmed that most of the respondents, despite being familiar with the term microplastics, are not well informed about their impact on the environment. The survey also indicates that this area lacks public awareness. Furthermore, a comparative analysis shows that more than 80% of the respondents would be in favour of refundable plastic bottles. Finally, the survey appears to confirm that respondents have poorer knowledge in legal regulations dealing with plastic waste.
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