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Magnetic Nanoparticles for Targeted Delivery System of Plasmid into HEK293 cells
Bílek, Ondřej ; Svoboda, Ondřej (referee) ; Fohlerová, Zdenka (advisor)
This bachelor’s thesis deals with using of magnetic nanoparticles, SPIO nanoparticles and nanoparticles of commercial product MATra for transfection of ASAP plasmid into HEK293 cells and subsequent optimization of the process. The experimental part is enclosed with theorethical part that summarizes findings from the fields of synthesis of magnetic nanoparticles and their biomedical applications, theory dealing with possibilities of the insertion of foreign DNA or plasmids into the cell and of its efficiency evaluation.
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The support of czech nonprofit sector by dutch foundations and organizations
Jamrichová, Hana ; Moree, Dana (advisor) ; Dohnalová, Marie (referee)
Diploma thesis "The support of Czech nonprofit sector by Dutch foundations and organizations" deals with a help and support of transforming society in 1989. It describes the civil sector in 1989, forms of financing non-profit organizations and deals with the role of foreign aid. Theoretical part contains a chapter about the Netherlands, the history of relations with this country as well as a focus on Dutch development assistance and its evaluation. The list of Dutch foundations and organizations that supported Czech civil sector is included. The research focuses on the MATRA/KAP program. The aim of research was to find out, which organizations were supported and what criteria have been established for obtaining support. I was also interested about experiences of donor and supported organizations.
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The support of czech nonprofit sector by dutch foundations and organizations
Jamrichová, Hana ; Moree, Dana (advisor) ; Dohnalová, Marie (referee)
Diploma thesis "The support of Czech nonprofit sector by Dutch foundations and organizations" deals with a help and support of transforming society in 1989. It describes the civil sector in 1989, forms of financing non-profit organizations and deals with the role of foreign aid. Theoretical part contains a chapter about the Netherlands, the history of relations with this country as well as a focus on Dutch development assistance and its evaluation. The list of Dutch foundations and organizations that supported Czech civil sector is included. The research focuses on the MATRA/KAP program. The aim of research was to find out, which organizations were supported and what criteria have been established for obtaining support. I was also interested about experiences of donor and supported organizations.
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Magnetic Nanoparticles for Targeted Delivery System of Plasmid into HEK293 cells
Bílek, Ondřej ; Svoboda, Ondřej (referee) ; Fohlerová, Zdenka (advisor)
This bachelor’s thesis deals with using of magnetic nanoparticles, SPIO nanoparticles and nanoparticles of commercial product MATra for transfection of ASAP plasmid into HEK293 cells and subsequent optimization of the process. The experimental part is enclosed with theorethical part that summarizes findings from the fields of synthesis of magnetic nanoparticles and their biomedical applications, theory dealing with possibilities of the insertion of foreign DNA or plasmids into the cell and of its efficiency evaluation.
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