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Kidney Transplantation: Donor-Recipient Pairing in University Hospital Hradec Králové
Moravcová, Lucie ; Řeháček, Vít (advisor) ; Jílek, Petr (referee)
8 ABSTRACT Author: Bc. Lucie Moravcová Supervisor: MUDr. Vít Řeháček Charles University, Faculty of Pharmacy in Hradec Králové Title of master's thesis: Kidney transplantation: donor-recipient pairing in University Hospital Hradec Králové Background: The aim of this study was to determine HLA, blood group, age and sex match in donor-recipient pairing in kidney transplantation. HLA alleles of deceased donors were typed in Transfusion Department of the University Hospital Hradec Králové. Methods: Donor's HLA-A*, HLA-B* and HLA-DRB1* alleles were typed by PCR - SSP method. Complex data evaluating and processing was then performed. Results: 97 deceased donors were tested between 2013 and 2018. A total of 98 kidneys received from them were subsequently transplanted to 98 recipients in University Hospital Hradec Králové. 60,2 % of the donors were men, 63,3 % of the recipients were men. Most of the donors, as well as the recipients, were 51-70 years old (50,0 % and 59,2 %, respectively). The most common diagnoses in the group of deceased donors were associated with brain damage (66,3 %), the most common cause of renal failure in the group of recipients was chronic inflammatory kidney disease (41,8 %). All 98 transplantations (100,0 %) were AB0 compatible. 74 transplantations (75,5 %) were RhD compatible. 5...
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The Frequency of HLA Antigens in the Group of Plasma Donors of the Transfusion Department, University Hospital, Hradec Králové
Nováková, Petra ; Řeháček, Vít (advisor) ; Jílek, Petr (referee)
Author: Petra Novakova Title: The frequency of HLA antigens in the group of plasma donor of the Transfusion Departmen, University Hospital, Hradec Králové Bachelor thesis Charles University, Faculty of Pharmacy in Hradec Kralove Field of study: Medical Laboratory Technician Background: The major objective of this study is the statistical data processing in order to determine the frequency of HLA antigens in the group of plasma donors. Methods: The serological typing of HLA antigens using the lymphocytotoxic test was performed among plasma donors of the Transfusion Department of the University Hospital Hradec Kralove. On a sample group of 200 plasma donors was determined the frequency of HLA antigens by blood group and gender on the basis of a serological examination. The collected data of the observed sample of aforementioned group were processed using Microsoft Excel in relation to the gender and blood group of the donor. Results: It was discovered that within the observed sample there is the highest frequency of HLA-A antigens 2, 1 and 3; HLA-B antigens 7, 44, 35 and HLA-C antigens 7, 6, 4. The most frequented combination of HLA antigens within the observed sample of 200 plasma donors regardless blood group and gender are combinations of HLA-A 1,2 (15x) HLA-A 2,3 (15x), HLA-B 8,44 (8x), HLA-B 7,...
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The role of immune system in autism
Niederlová, Veronika ; Otáhal, Jakub (advisor) ; Hájková, Michaela (referee)
Autism is a complex neurodevelopmental disorder with yet unknown etiology. The research point out abnormalities in the immune system of pacients with autism, which could be related to the development of the disease. This thesis reviews current scientific knowledge about the role of the immune system in the development of autism. The leading chapters include family history of autoimmune diseases, specific HLA alleles, the most common autoantibodies and maternal antibodies, lowered NK cell activity and cytokine levels in pacients with autism. Powered by TCPDF (www.tcpdf.org)
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Occurrence of HLA-B27 antigen in patients examined for suspected rheumatological diseases
Lipenská, Zuzana ; Řeháček, Vít (advisor) ; Jílek, Petr (referee)
Author: Zuzana Lipenská Title: Occurrence of HLA-B27 antigen in patients examined for suspected rheumatological diseases Bachelor's Thesis Charles University in Prague, Faculty of Pharmacy in Hradec Kralove Field of study: Medical Laboratory Technician Background: The aim of the study of HLA B27 antigen in patients investigated for suspected rheumatological disease in 2015. Methods: Patient samples examined for the presence of antigen HLA-B27 in the HLA laboratory of the Transfusion Department, University Hospital Hradec Kralove are processed using the serological method. First is used the isolation of peripheral blood lymphocytes using a density gradient, thereafter lymphotoxical test with peripheral lymphocytes. Results: In 2015 were examined 1,329 patients. 207 persons had the HLA-B27, 1122 persons did not. Of the total number of examined patients were examined 754 women and 575 men. Positive was found 108 women and 99 men. The highest incidence of HLA-B27 was observed in the group of 20-29 years old, another slight increase was in the group of 10-19 years old and 40-49 years old. The examined persons had mainly a group diagnosis of disease of the musculoskeletal system with the most frequently diagnosed dorsalgia (M54). Conclusions: The association between HLA-B27 antigen and the deseases has...
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