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Optimization of isolation and PCR methods for the identification of CMS in onion
PETERKA, Václav
This bachelor thesis deals with the optimization of different methods of DNA isolation from different types of onion (Allium cepa L.) samples and the subsequent detection of the Ms locus and the detection of cytoplasmic male sterility (CMS). The different types of DNA extractions performed and their results were compared and evaluated. The best quality DNA was isolated from leaves using the CTAB-PVP. These test samples were also used for PCR for the detection of CMS and Ms locus using several different markers, which were compared with each other. The most suitable markers were then used in the PCR for the main samples. DNA isolation by CTAB-PVP was also used for further DNA isolation from the main samples of onion, which were two sterile lines and four fertile lines. The extracted DNA from these samples was first used to detect the Ms locus. Detection was performed using the marker AcSKP1. The samples were collected over several weeks. The Ms locus was detected in all samples (C1C6), regardless of the age of the plant. However, the Ms locus was detected in various forms of zygosity in samples from lines designated C2, C4 and C5. Furthermore, the DNA samples were used to detect CMS in plants and the type of CMS was determined. The markers cob and orfA501were used for this detection. Using these markers, it was found that the lines that were declared sterile (lines designated C1 and C4) were indeed sterile and had the CMS-T sterility type. The lines C2, C3 and C6 that were labelled as fertile were also detected as sterile samples. This means that these lines are not 100% fertile. In the line designated as C5, no sterility was detected in any of the samples.
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Production of cooling cabinet
Halabrín, Marek ; Peterková, Eva (referee) ; Císařová, Michaela (advisor)
The thesis deals with presenting an assembly proposal of a cooling cabinet. The cooling cabinet is made from an austenitic steel plate of the ČSN 17 240 grade with a thickness of 1.5 millimetres. The assembly proposal is composed according to the design documentation. The number of the assembled pieces is 200. The manufacturing shall be made with the use of bending and punching. These manufacturing techniques form a part of this thesis. Finn power C5 with a nominal force of 300 kN and Trubend 5170 with a nominal force of 1700kN are the machines chosen for the manufacturing process. These are standardized machines made from a quenched steel and a steel of the 42CrMo4 type. A technological-economic evaluation comparing expenses of the manufacturing also forms a part of the thesis as well.
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Vliv vazného ustájení na parametry mléčné užitkovosti dojnic ve vybraném chovu
Skoupý, Roman
The aim of this work was to find out the influence of bonded housing on the parameters of milk productivity of dairy cows in a selected farm. The work took place in one calendar year 2022 from January to December. Among the monitored parameters were fat content (%), protein (%), lactose (%), amount of milk produced (kg), stage of lactation (days) and order of lactation (n). The samples were obtained from our own breeding and evaluated in the Laboratory of Applied Lactology at the Institute of Animal Breeding and Breeding, Faculty of Agriculture, Mendel University in Brno using the Julie C5 Automatic device. A total of 12 samples from 4 cows of two breeds, namely Holstein and Czech Spotted cattle, were processed. The results show that bonded housing has a significant effect on the milk production of a dairy cow. The fat content is influenced by the method of breeding but also the feeding technique and the health status of dairy cows, when the fat content was very different in individual months. The protein content was influenced by the order of lactation, where the higher the lactation, the lower the protein content. Lactose was not affected by any factor and was constant.
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Effects of proprioceptive neuromuscular facilitation on the upper limb function of people with C4-C5 spinal cord injury
Halamová, Adéla ; Zvelebilová, Pavlína (advisor) ; Melicharová, Hana (referee)
Title: Effects of proprioceptive neuromuscular facilitation on the upper limb function of people with C4-C5 spinal cord injury The main objective: The aim of the work was to find out what effect the use of the proprioceptive neuromuscular facilitation method will have on the function, muscle volume and ranges of movements of the upper limb in people who are affected by a spinal cord lesion in the C4 and C5 region. Furthermore, the work dealt with the subjective feeling of the patients, whether the upper extremity improved after the therapy and if it was possible to involve it more in ADL activities. Methods: 5 probands took part in the study - all men with an average age of 41 years. A total of 3 measurements were performed for the study participants - approx. 2 months before the start of the intervention, before the start of the therapy and after the end. Values such as the volume of the arm muscles in contraction, the range of motion of abduction in the shoulder joint and flexion in the elbow joint were recorded. Another measured subject was the forearm endurance test. Participants also filled out the SCIM form before and after graduation. Probands practiced the method of proprioceptive neuromuscular facilitation of the 1st diagonal in addition to their regular therapy. They were also instructed...
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Methodology for specific cloud certifications
Vyšatová, Simona ; Adam,, Kučínský (referee) ; Sedlák, Petr (advisor)
The master thesis deals with the topic of cloud certifications. Its main goal is to create a methodological material for the National Cyber and Information Security Agency (NUKIB) and the general public, which summarizes the basic differences between the most important cloud certifications and compares with the European Cloud Certification Scheme – Cybersecurity Certification Scheme for Cloud Services (EUCS). The thesis defines basic concepts related to cloud computing, introduces individual cloud schemes (EUCS, SOC 2, C5, eGovernment cloud), key players in the field of cloud computing regulation and certification and the most important cloud service providers. Subsequently, a practical comparison of the presented cloud certifications is made and some impacts of the adoption of EUCS are analysed. The selected differences will serve as a source for further decision making for the NUKIB when working with the EUCS scheme.
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Functional independence measures in wheelchair rugby players
Moudrá, Kristýna ; Vařeková, Jitka (advisor) ; Pavlová, Ilona (referee)
Title: Functional independence measures in wheelchair rugby players Objectives: The aim of the thesis is to investigate the relationship between the sports activity of wheelchair rugby players and the degree of functional independence in daily activities in people with spinal cord injury. Methods: 20 male wheelchair rugby players aged between 20 and 51 years, in the range of spinal cord injury level C5 - Th8, were selected for the empirical mixed research using the purposive sampling method. The methods of data collection include an interview and a self-constructed questionnaire that elicits anamnestic data, subjective assessment of self- sufficiency and player's experiences with health complications associated with wheelchair rugby. Furthermore, an evaluation of the SCIM III functional degree of independence was carried out, the results of which were compared with the results of two previous studies (EMSCI and assumptions of Occupational Therapists from the Czech Republic). Results: The results confirm a positive relationship between the player's functional independence and the sports activity of wheelchair rugby players. Respondents at levels of complete spinal cord injury C6 and C7-8 with the SCIM III assessment demonstrated a higher functional independence rate compared to the overall average...
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Investigation of monomolecular organic layers and their interaction with atmospheric oxidants and pollutants
Habartová, Alena ; Jungwirth, Pavel (advisor) ; Jedlovszky, Pal (referee) ; Klán, Petr (referee)
Title: Investigation of monomolecular organic layers and their interaction with atmospheric oxidants and polutants Author: Mgr. Alena Habartová Institute: Institute of Organic Chemistry and Biochemistry, Academy of Sciences of the Czech Republic Advisor: prof. Mgr. Pavel Jungwirth, DSc. Abstract: Using classical molecular dynamics simulations, we studied interac- tions between Langmuir monolayers of palmitic acid (PA) and haloalkanes as serious atmospheric pollutants, to model naturally occurring and atmospherical- ly relevant complex surfaces of extended water bodies and aqueous aerosols. We investigated partitioning, orientation, solvation, as well as structure and morphol- ogy of the individual components and their mixture at water/vapor interfaces at different temperatures in order to validate our computational model and com- plement experimental data with molecular-level details. First, we have shown that halocarbon molecules adsorbed on amorphous ice nanoparticles at 100 K remain mostly isolated and do not form aggregates, in contrast to their cluste- ring on cryogenic argon nanoparticles. Next, we report that chloro-, bromo-, and iodoalkanes with short alkyl chains (up to C5) exhibit high preference for the outermost region of the water/vapor interface. Their average residence times of several...
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Direct Activation of the C-H Bond in Tetrazoles
Lubojacký, Richard ; Pour, Milan (advisor) ; Hrabálek, Alexandr (referee)
Direct activation of C-H bond in tetrazoles Richard Lubojacký, 5th grade Supervisor: Prof. RNDr. Milan Pour, Ph.D. Department of Inorganic and Organic Chemistry, Faculty of Pharmacy in Hradec Králové Within the framework of this diploma thesis, direct arylation and alkenylation of 1- substituted tetrazoles at C5 was developed and optimized. An economical preparation of a range of 1,5-disubstituted tetrazoles was thus made possible. In these reactions, Pd-catalysis in the presence of CuI and Cs2CO3 was found as optimal. Contrary to similar reactions of imidazoles and purines, it was necessary to incorporate phosphine ligands in order to stabilize Pd - intermediates and prevent them from a premature decomposition into cyanamides. A library of 1,5-disubstituted tetrazoles with very promising yields has been prepared using this method.
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MD simulations of complexes between nucleic acids and RNase H
Bartek, Tomáš ; Barvík, Ivan (advisor) ; Burda, Jaroslav (referee)
The aim of this diploma thesis was to study interactions between human Rase H enzyme and a natural and modified substrate using molecular dynamics simulations (altogether 9 MD runs ere produced). Conformational preferences of internucleotide linkages (undergoing contacts with the RNase H enzyme) were studied using several versions of the AMBER force field. Either one or two copies of RNase H were included into the simulated system. As the most important DNA-binding residues were recognized Trp93 and Ser101 in the first DNA binding site and Thr49 and Arg47 in the second DNA binding site. Further, the AMBER force field was re-parameterized slightly using ab initio calculations to produce force constants for the modified phosphonate internucleotide linkage. Biologically active version of the modified internucleotide linkage C3-O3-P-C-O5-C5 was able to bind Arg47 using two hydrogen bonds within the 10 ns MD run (even more effciently than in the case of MD runs with natural internucleotide linkages). On the other hand, the biologically inactive C3-O3-C-P-O5-C5 internucleotide linkage lose contacts with Arg47 quickly.
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Decision trees
Jahoda, Miroslav ; Mrázová, Iveta (advisor) ; Gregor, Petr (referee)
Among the known methods of data mining are neural networks, ILP, associative rules, Bayes networks, clustering, decision trees and others. This thesis is about decision trees, their implementation, visualization, extraction rules and the comparison of different decision trees and models of classification data in general. An integral part of the data mining process is preprocessing of data, which plays an important role and is also discussed in this thesis. Part of this thesis also concerns the comparison of different decision tree models such as CART, CHAID, C5.0 (See5) and others on a set of 3 data kinds. Finally, this model's results are compared to its results when data preprocessed by PCA analysis is used.
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