|
|
Nanosafety and the Current Trends in the Risk Assessment of Nanoparticles
Valovič, Stela ; Poláčik, Ján (referee) ; Adamec, Vladimír (advisor)
This diploma thesis deals with the current state of nanosafety. The aim of this paper was to review and critically analyze approaches to facilitate the risk assassment of nanomaterials. In the first part it provides an overview of the most important methods and software tools that are used in this field. Methods were analyzed against a set of relevant criteria. Criteria include consideration of exposure, life cycle and physicochemical properties, transparency, applicability and the field of application. Different criteria were chosen for the software tools. Based on results, a new approach was suggested. The approach takes the form of a diagram based on questions. The purpose of the suggested approach is to recommend the most relevant method, tool or their combination to assess the safety of a nanomaterial depending on known characteristics. The approach was also implemented in a software tool.
|
|
|
Advanced microscopy in the research of colloidal systems
Valovič, Stela ; Venerová, Tereza (referee) ; Mravec, Filip (advisor)
This bachelor thesis is focused on dual-colour fluorescence cross-correlation spectroscopy. As a suitable combinations of fluorescenct dyes were designated this combinations: perylene + nile red, perylene + DiD and DiO + DiD. The funtionality of this method was veried by tetraspeck. The experiment of FCCS was performed on micellar surfactants solitions which proved to be unsuitable for this method. Consequently, liposomes with DiD and DiO incorporated in phospholipid bilayer were measured. The cross-correlation in liposomes was succesfuly measured.
|
|
|
Influence of particle size on microreology experiments using fluorescence correlation spectroscopy
Valovič, Stela ; Sedláček, Petr (referee) ; Mravec, Filip (advisor)
This diploma thesis deals with microrheology measured via the fluorescence correlation spectroscopy. As microrheological probes, fluorescently marked nanoparticles of 5 sizes in the range of 10-100 nm, were used. The particles had been immersed in a variety of concentrated glycerol solutions and agarose gels of different concentrations, and the FCS measurement revealed a diffusion coefficient of individual particles in each environment. Based on the coefficient, the viscosity of the glycerol needed to stop the particles could be determined. Particles of 10 nm size were not stopped even by the 100 wt% glycerol. In the case of the agarose gels, a combination of higher agarose concentration and larger particles resulted in an increase in the diffusion coefficient to an unlikely high value. This was caused probably by an agarose autofluorescence and the value indicates stopping of the particles in the given agarose gel. Later, the data acquired by the FCS measurement were converted to MSD curves using MATLAB software. The thesis discusses the influence of the experimental parameters on the shape of the MSD curve. The results showed that the number of particles and autocorrelation function have the most significant effect.
|
|
|
Nanosafety and the Current Trends in the Risk Assessment of Nanoparticles
Valovič, Stela ; Poláčik, Ján (referee) ; Adamec, Vladimír (advisor)
This diploma thesis deals with the current state of nanosafety. The aim of this paper was to review and critically analyze approaches to facilitate the risk assassment of nanomaterials. In the first part it provides an overview of the most important methods and software tools that are used in this field. Methods were analyzed against a set of relevant criteria. Criteria include consideration of exposure, life cycle and physicochemical properties, transparency, applicability and the field of application. Different criteria were chosen for the software tools. Based on results, a new approach was suggested. The approach takes the form of a diagram based on questions. The purpose of the suggested approach is to recommend the most relevant method, tool or their combination to assess the safety of a nanomaterial depending on known characteristics. The approach was also implemented in a software tool.
|
|
|
Influence of particle size on microreology experiments using fluorescence correlation spectroscopy
Valovič, Stela ; Sedláček, Petr (referee) ; Mravec, Filip (advisor)
This diploma thesis deals with microrheology measured via the fluorescence correlation spectroscopy. As microrheological probes, fluorescently marked nanoparticles of 5 sizes in the range of 10-100 nm, were used. The particles had been immersed in a variety of concentrated glycerol solutions and agarose gels of different concentrations, and the FCS measurement revealed a diffusion coefficient of individual particles in each environment. Based on the coefficient, the viscosity of the glycerol needed to stop the particles could be determined. Particles of 10 nm size were not stopped even by the 100 wt% glycerol. In the case of the agarose gels, a combination of higher agarose concentration and larger particles resulted in an increase in the diffusion coefficient to an unlikely high value. This was caused probably by an agarose autofluorescence and the value indicates stopping of the particles in the given agarose gel. Later, the data acquired by the FCS measurement were converted to MSD curves using MATLAB software. The thesis discusses the influence of the experimental parameters on the shape of the MSD curve. The results showed that the number of particles and autocorrelation function have the most significant effect.
|
|
|
Advanced microscopy in the research of colloidal systems
Valovič, Stela ; Venerová, Tereza (referee) ; Mravec, Filip (advisor)
This bachelor thesis is focused on dual-colour fluorescence cross-correlation spectroscopy. As a suitable combinations of fluorescenct dyes were designated this combinations: perylene + nile red, perylene + DiD and DiO + DiD. The funtionality of this method was veried by tetraspeck. The experiment of FCCS was performed on micellar surfactants solitions which proved to be unsuitable for this method. Consequently, liposomes with DiD and DiO incorporated in phospholipid bilayer were measured. The cross-correlation in liposomes was succesfuly measured.
|
guest ::
