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Preparation and characterization of hydrogels with diffusion gradient
Tvrdoňová, Anna ; Pekař, Miloslav (referee) ; Smilek, Jiří (advisor)
The presented bachelor thesis deals with the preparation and further characterization of hydrogels with a diffusion gradient. The aim of the thesis is a literature research on the issue of hydrogels with a diffusion gradient, and a selection of a suitable method for its preparation. Moreover, conducted pilot experiments lead to the characterization of the properties of the prepared systems, especially with regard to proving the gradient structure of hydrogels. The performed evaluation was based on visual observation, determination of transport, and viscoelastic properties. In addition, a simple method consisting of weighing the prepared hydrogels was used. Hydrogels were compared depending on the choice of crosslinking agent, its concentration, and crosslinking time. Gradient hydrogels based on polysaccharide sodium alginate were prepared. The results suggest that by selecting the concentration of the crosslinking agent, as well as the crosslinking time, the final properties (viscoelastic and transport) of the hydrogels can be adjusted to the desired values. In the discussion of the results, we propose steps for a more comprehensive view of the issue and providing options for the following research.
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Semiconductor microstructures for cellular photostimulation
Tvrdoňová, Anna ; Weiter, Martin (referee) ; Glowacki, Eric Daniel (advisor)
Peroxid vodíku (H2O2) je metastabilní reaktivní forma kyslíku, která reguluje mnoho biologických drah. Úloha H2O2 v biologických procesech závisí na jeho lokální koncentraci, v malém množství (nM) může působit jako signální molekula, zatímco vyšší koncentrace mohou mít cytotoxické účinky. Cílem této práce bylo vytvoření fotosenzitivních mikročástic, které by produkovaly fyziologické množství peroxidu vodíku pro regulaci signálních drah v kultivovaných buňkách. Navrhované mikročástice jsou organické fotovoltaické struktury bez substrátu, které produkují H2O2 fotofaradickou redukcí molekulárního kyslíku. Produkce peroxidu probíhá za současné oxidace donoru elektronů přítomného v elektrolytu. Částice mají rozměry v řádu mikrometrů, zatímco jejich tloušťka nepřekračuje 100 nm. Fotofaradické reakce jsou poháněny červeným světlem (660 nm), které může pronikat tkáněmi, což umožňuje potenciální aplikace mikročástic in vivo. Práce se bude zabývat optimalizací výroby mikročástic a charakterizací produkce H2O2.
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Automutilation in childhood
Tvrdoňová, Anna ; Procházková, Jana (advisor) ; Soukupová, Tereza (referee)
This thesis deals with the topic of self-injury of adolescents. Its goal is to analyze the adolescents' view of self-injury, how the youth themselves define self-injury and what they perceive to be self-injurious behavior. The work is divided into two core parts. The first section focuses on the definition of the self-injury and reviews both current Czech and international literature. Particular attention is devoted to the inconsistent terminology, definitions and presentation of the recent classification system of selfinjury. The second part is devoted to the research of explorative character. The data have been collected through a survey using anonymous self report questionnaire from 73 pupils between the ages of 13-15 and they were analysed using categorization and the method of factor and cluster analysis. Factor analysis extracted ten factors which served as the meaningful units and can be interpreted as adolescents' viewpoints of self-injury. Findings suggest the adolescents consider not only direct destruction of one's own body as example self-injury but also behavior that indirectly leads to negative health outcomes (eating disorders, smoking, abuse of alcohol). Interestingly, the youth reported ambivalent viewpoint of self-injury. They reported not only the losses and the possible risks of such...
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Preparation and characterization of hydrogels with diffusion gradient
Tvrdoňová, Anna ; Pekař, Miloslav (referee) ; Smilek, Jiří (advisor)
The presented bachelor thesis deals with the preparation and further characterization of hydrogels with a diffusion gradient. The aim of the thesis is a literature research on the issue of hydrogels with a diffusion gradient, and a selection of a suitable method for its preparation. Moreover, conducted pilot experiments lead to the characterization of the properties of the prepared systems, especially with regard to proving the gradient structure of hydrogels. The performed evaluation was based on visual observation, determination of transport, and viscoelastic properties. In addition, a simple method consisting of weighing the prepared hydrogels was used. Hydrogels were compared depending on the choice of crosslinking agent, its concentration, and crosslinking time. Gradient hydrogels based on polysaccharide sodium alginate were prepared. The results suggest that by selecting the concentration of the crosslinking agent, as well as the crosslinking time, the final properties (viscoelastic and transport) of the hydrogels can be adjusted to the desired values. In the discussion of the results, we propose steps for a more comprehensive view of the issue and providing options for the following research.
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Self-harm in children
Tvrdoňová, Anna ; Procházková, Jana (advisor) ; Sotáková, Hana (referee)
Title: Self-harm in children Abstract This thesis deals with the topic of self-injury of adolescents. Its goal is to analyze the adolescents' view of self-injury, how the youth themselves define self-injury and what they perceive to be self-injurious behavior. The work is divided into two core parts. The first section focuses on the definition of the self-injury and reviews both current Czech and international literature. Particular attention is devoted to the inconsistent terminology, definitions and presentation of the recent classification system of self- injury. The second part is devoted to the research of explorative character. The data have been collected through a survey using anonymous self report questionnaire from 73 pupils between the ages of 13-15 and they were analysed using categorization and the method of factor and cluster analysis. Factor analysis extracted ten factors which served as the meaningful units and can be interpreted as adolescents' viewpoints of self- injury. Findings suggest the adolescents consider not only direct destruction of one's own body as example self-injury but also behavior that indirectly leads to negative health outcomes (eating disorders, smoking, abuse of alcohol). Interestingly, the youth reported ambivalent viewpoint of self-injury. They reported not only the...
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Self-harm in children
Tvrdoňová, Anna ; Procházková, Jana (advisor) ; Sotáková, Hana (referee)
Title: Self-harm in children Abstract This thesis deals with the topic of self-injury of adolescents. Its goal is to analyze the adolescents' view of self-injury, how the youth themselves define self-injury and what they perceive to be self-injurious behavior. The work is divided into two core parts. The first section focuses on the definition of the self-injury and reviews both current Czech and international literature. Particular attention is devoted to the inconsistent terminology, definitions and presentation of the recent classification system of self- injury. The second part is devoted to the research of explorative character. The data have been collected through a survey using anonymous self report questionnaire from 73 pupils between the ages of 13-15 and they were analysed using categorization and the method of factor and cluster analysis. Factor analysis extracted ten factors which served as the meaningful units and can be interpreted as adolescents' viewpoints of self- injury. Findings suggest the adolescents consider not only direct destruction of one's own body as example self-injury but also behavior that indirectly leads to negative health outcomes (eating disorders, smoking, abuse of alcohol). Interestingly, the youth reported ambivalent viewpoint of self-injury. They reported not only the...
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Automutilation in childhood
Tvrdoňová, Anna ; Procházková, Jana (advisor) ; Soukupová, Tereza (referee)
This thesis deals with the topic of self-injury of adolescents. Its goal is to analyze the adolescents' view of self-injury, how the youth themselves define self-injury and what they perceive to be self-injurious behavior. The work is divided into two core parts. The first section focuses on the definition of the self-injury and reviews both current Czech and international literature. Particular attention is devoted to the inconsistent terminology, definitions and presentation of the recent classification system of selfinjury. The second part is devoted to the research of explorative character. The data have been collected through a survey using anonymous self report questionnaire from 73 pupils between the ages of 13-15 and they were analysed using categorization and the method of factor and cluster analysis. Factor analysis extracted ten factors which served as the meaningful units and can be interpreted as adolescents' viewpoints of self-injury. Findings suggest the adolescents consider not only direct destruction of one's own body as example self-injury but also behavior that indirectly leads to negative health outcomes (eating disorders, smoking, abuse of alcohol). Interestingly, the youth reported ambivalent viewpoint of self-injury. They reported not only the losses and the possible risks of such...
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