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Study of cardiomyocytes viability using confocal microscopy
Chutný, Pavel ; Svoboda, Ondřej (referee) ; Čmiel, Vratislav (advisor)
This work describes the study of cardiomyocytes viability using confocal microscopy. The theoretic introduction covers the basic principle of fluorescence together with fluoresceins in cells biology. The struction of cardiomyocytes, their selection from the lively heart and the methods of viability examination is comprised in the next part. The third topic is about confocal mikroskopy and its posibilities in cardiomyocytes imaging. In next chapters are described the experiments and their evaluating.
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Evaluation of Automatic Vessel Tree Segmentation Algorithms
Svoboda, Ondřej ; Jan, Jiří (referee) ; Odstrčilík, Jan (advisor)
Segmentation of the vasculature is an important step in the process of the retinal image analysis. The results of the analysis can be used to diagnose several eye and cardiovascular diseases. This work deals with the creation of gold standard database of high resolution retinal images and their use in evaluating the success of vascular automatic segmentation methods. The aim is to create the application, which will online evaluate the success of the automatic vessel segmentation methods. In brief, this work describes the characteristics of image data from digital fundus camera, the method of image segmentation and automatic segmentation methods of blood vessels. Furthermore, this work describes the gold standard, the databases of gold standards and ultimately the properties of the new database and the reason for HRF (High Resolution Fundus Images). The last chapter deals with methods of evaluating the success of vascular automatic detection methods and application development for this assessment.
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Magnetic Nanoparticles for Targeted Delivery System of Plasmid into HEK293 cells
Bílek, Ondřej ; Svoboda, Ondřej (referee) ; Fohlerová, Zdenka (advisor)
This bachelor’s thesis deals with using of magnetic nanoparticles, SPIO nanoparticles and nanoparticles of commercial product MATra for transfection of ASAP plasmid into HEK293 cells and subsequent optimization of the process. The experimental part is enclosed with theorethical part that summarizes findings from the fields of synthesis of magnetic nanoparticles and their biomedical applications, theory dealing with possibilities of the insertion of foreign DNA or plasmids into the cell and of its efficiency evaluation.
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Application of fluorescence staining for the "Scratch Wound Healing Assay" experiment
Zumberg, Inna ; Svoboda, Ondřej (referee) ; Chmelíková, Larisa (advisor)
This bachelor’s thesis studies the cell culture of 3T3 cell line (mouse embryonic fibroblast cells) and cell migration. The Scratch Wound Healing Assay was used for the study of cell migration. The basic principles of experimenting, its advantages and disadvantages, as well as the practical use of this method in scientific research were presented on specific examples. Practical part of the bachelor’s thesis describes the experiment in the cell laboratory including cell culture of 3T3 cell line. The proposed experiment was tested with a sufficient number of repetitions. The statistical processing of microscopic images was performed in the MATLAB programming environment.
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Proposal of Implementation of Business Strategy with IS/IT Support
Svoboda, Ondřej ; Polák, Vojtěch (referee) ; Zich, Robert (advisor)
This master's thesis deals with a implementation of IT/IS tools to support the implementation of the company's business strategy, which sells and provides after sales servicematerial handling equipment. The thesis examines key internal and external factors related to the implementation of business strategy of the company, evaluates them and proposes concrete implementation of IT/IS tools to support achievement of strategic goals.
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GPU Implementation of Resampling Antialiasing
Svoboda, Ondřej ; Lysek, Tomáš (referee) ; Starka, Tomáš (advisor)
The aim of this thesis is the implementation of resampling antialiasing for OpenGL. This thesis is based on already existing solution for DirectX. We are introducing several antialiasing methods used in todays graphical aplications. Next we are describing application implementation with differences against the original solution. Our implementation of resampling antialiasing was compared with other antialiasing methods and with the original solution. Image quality and performace were evaluated.
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Usage of Busbar as a Source of Heat
Svoboda, Ondřej ; Macháček, Jan (referee) ; Radil, Lukáš (advisor)
This study is dedicated to the use of heat energy from the busbar and its cooling system. The study deals with thermal analysis of the busbar, both using numerical simulations in SolidWorks and practical examination – warming of the busbar. We can verify the correctness of the simulation from the measured values. To determine the temperature profile of the busbar, it is necessary to measure the temperature at various points of the busbar by contact method using thermocouple or contactless method using a thermal imager. Measurements are performed at the busbar LIA1600 made by Siemens Busbar Trunking System Mohelnice. The aim of the study is to analyze temperature busbar, to suggest the most efficient method of heat recovery, and to make economic calculation
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Quantitive optical analysis using cell phone
Petlák, Daniel ; Svoboda, Ondřej (referee) ; Čmiel, Vratislav (advisor)
This bachelor‘s thesis discusses methods for the quantitative colorimetric analysis using a cell phone and a design of my own analyzer. Theoretical part deals with basic characteristics of light and division of optical spectral analysis, which is described by several optical methods. The bachelor’s thesis also contains the division of colorimetric analyzers according to their types. The most important part is focused on a colorimetric analysis using the cell phone and familiar with the possibilities of analysis of liquids and solid samples. The practical part deals with the design of a separate analyzer using the cell phone, developed application including the possibility of calibration values of prepared samples and subsequent evaluation of ion concentration in unknown sample. For the accuracy of the results is made dark light-tight cover printed by 3D printer.
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Stabilization of the flooded sand pit near Hulín
Tomšej, Lukáš ; Svoboda, Ondřej (referee) ; Šlezingr, Miloslav (advisor)
The diploma thesis assesses the current state of the Hulín gravel and sand plant and pays attention to abrasions of severely damaged banks of the reservoir. With the help of the design of a suitable biotechnical stabilization, the receding of the shoreline in the section of interest will be prevented. The basis for the processing of appropriate bank stabilization proposals is the results achieved using a method of determining shore retreat. The individual developed variants of the bank remediation solution emphasize the use of vegetation support, especially suitable bank stands to ensure the stability of the banks and protection against abrasion.
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