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Analysis of mastitis occurrence on chosen farm
Vařeka, Jan ; Stádník, Luděk (advisor)
Mastitida je zánět mléčné žlázy způsobený mikroorganismy, které produkují toxiny nebezpečné pro živý organismus. Bakalářská práce pojednává o vzniku, projevech, původcích, diagnostice, léčbě, a prevenci mastitid. Ve druhé části je představena úroveň mastitid na farmě Šedivec. Zdravotní stav mléčné žlázy se především odvíjí od celkové zoohygieny a welfare chovu. S projevením zánětu přichází odpovědnost správné diagnózy a léčby. Mastitidy se řadí mezi nejvýznamnějších onemocnění mléčného skotu. Ekonomické ztráty pro chovatele zahrnují přímé náklady na léčiva a nepřímé způsobené ztrátou mléka poklesem dojivosti či zkrácenou laktací, sníženou reprodukcí, vyšší náchylnosti k další infekci, zvýšenou brakací a pracovní náročností.
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Identification of production indicators important for definition of the selection criteria in cattle breeding.
Michaličková, Monika ; Vostrý, Luboš (advisor) ; Stádník, Luděk (referee)
The aim of the study was to apply all available information (for time period 2011 to 2013) about the dual purpose cattle breeds (Simmental and Pinzgauer) to the program package ECOWEIGHT and to suggest selection criteria for Slovak dairy and cow calf production systems. The main advantages of Ph.D. thesis were the calculation of economic weights with comprehensive consideration and updating of the current farming conditions, as well as the calculation of economic weights for new traits i. e. health and residual feed intake traits. For the calculations, the bio-economic model and the most common marketing strategy for given production system were used. The main comparative advantage of Simmental and Pinzgauer breeds, were the milk yield (dairy system) and proportion of sold calves per 100 cows (cow-calf system), respectively. For the both breeds and production systems the longevity of cows was detected as the weakness. The highest proportion in cost and revenues structure for the feeding and main products was observed, respectively. For both breeds and production systems, the profit (regardless of subsidies including) was not reached. For Simmental breed in dairy and cow calf system, the highest economic importance was observed for milk yield (19.9%) and fertility of cows (22.5%), daily gain of calves till weaning (age of 100 days; 16.1%) and weight of calves at 210 days of age (14.3%) and for the longevity of cows (11.4% and 12.4%), respectively. On the other hand, for SP breed the highest relative importance was observed for daily gain till weaning (100 days of age, 20.3%) and weight of calves at 210 days (100 days of age; 20.3%), milk yield (20.2%) and weight at 120 days of age (14.4%) and for longevity of cows (10.1% and 16.8%) in dairy and cow-calf population, respectively. Based on the results of Ph.D. thesis, it could be recommended to reconsider the current breeding objectives, as well as to redefine the selection indices of dual-purpose cattle breeds in Slovakia. The emphasise should be based to growth and functional traits, with the aim to integrate preferences of consumers (environmental criterion and popularity of animal products), as well as to ensure the sustainability of animal production.
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Freezing technology of bull sperm in relation to its survivability and fertilization ability
Doležalová, Martina ; Stádník, Luděk (advisor) ; Jiří, Jiří (referee)
The aim of optimalization the insemination doses production is to provide the highest fertilization ability of spermatozoa during the demanding proces of processing fresh semen and its subsequent cryopreservation. Temperature changes causes spermatozoa damage during the cooling and freezing. Spermatozoa is exposed to cold shock and many others limiting factors, which leads to cell death and therefore to decline of fertilization ability of thawed insemination doses. For increasing spermatozoa resistance, exactly the plasma membrane resistance against cold shock was fraction of egg yolk LDL cholesterol (low density lipoprotein) at various concentrations into the comercially produced diluents added. It is believed that LDL acts possitively to plasma membrane and helps to maintain the fertilization ability of spermatozoa after thawing. Following step in the proces of insemination doses production is slow cooling of diluted semen and equilibration, when the straws are store at cooling box for 30 minutes to 240 hours. This period is necessary to penetrate of certain diluent components into the spermatazoa also maintain the balance between their intracellular and extracellular concentration. Also important is subsequent freezing temperature gradient of insemination doses. The most suitable freezing method is based on computer controlled temperature decline in freezing chamber which allows the precise control of ice crystals formation that could tear and kill the cell.
During 2012 to 2016 was repeatedly collected semen from the group of breeding bulls (n = 27, Holstein and Czech Fleckvieh breed) at AI centre. Semen which fulfill the standard entrance conditions in first step was evenly into several parts divided. For dilution the three types of comercially diluents AndroMed, Bioxcell and Triladyl with and without LDL addition were used. Into the diluents AndroMed and Bioxcell the concentration of LDL 4 %, 6 % and 8% into the dilent Triladyl 6 %, 8 % and 10 % was added. Diluted semen was filled into the glass capillares with volume 0,1 ml and temperature +4 °C. Subsequently the sample was placed to cold bath (0°C) for 10 minutes. Then the volume of capillare with physiological solution (37 °C) was mixed and for next 120 minutes was incubate. The effect of cold shock to proportion of live spermatozoa was evaluated by using Eosin and Nigrosine staining technique during heat test of spermatozoa survivability after spermatozoa heating and after 120 minutes of incubation.
The more suitable semen diluents which provide the higher spermatozoa resistance against cold shock were AndroMed and Bioxcell. Together the possitive effect of LDL addition into the diluents to lower decrease of proportion of live spermatozoa during heat test was found (P<0.05). The most suitable LDL concentration which had a favorable influence at spermatozoa resistance against cold shock was 6 % in diluent Bioxcell. Values of the proportion of live sperm were higher at the beginning of the heat test (+1.31% to + 3.2%) and after 120 minute incubation (+5.82% to +8.41%) compared to other diluents with and without addition of LDL.
In the next step the process of equilibration was optimized, is an important part of insemination doses production. The effect of the length of equilibration for subsequent fertilization ability of spermatozoa was evaluated using spermatozoa motility based of CASA and proportion of live spermatozoa after thawing and during heat survival test lasting 120 minutes (37 ° C). Suitable semen was diluted by comercially used diluent AndroMed based on soya lecithin, filled into the straws (0.25 ml), cooled and equilibrated in cooling box for 30, 120 and 240 minutes and freezed in programmable freezing box applying four types of freezing curves differing in temperature rate decline. There was used standard and by producer recommended 3. phase freezing curve, then 2. phase freezing curve, and 3. phase freezing curve with slower as well as rapid decline of temperature rate in freezing chamber, compared with standard freezing curve. The highest spermatozoa motility was found using 240 minutes of equilibration by +2.72% and +4.58% compared to other lengths of equilibration (P <0.05 to 0.01). The highest proportion of live spermatozoa was found using 120 minutes of equilibration (+6.87 % and +8.68 %). The highest average spermatozoa motility during heat test after thawing was achieved by using 2. phase freezing curve (from +2.97% to +10.37%, P <0.05), also in the proportion of live spermatozoa (from + 4.37% to +8.82%, P <0.01). When evaluating interaction between the length of equilibration and freezing curve (standard 3. phase and 2 . phase freezing curve), the highest average spermatozoa motility and proportion of live spermatozoa using 240 minutes of equilibration by both freezing curves was reached, there was no statistically significant differences. As well as, in all evaluated parts of this study the individual differences between ejaculate of bulls and within semen from one bull (P <0.05) as secondary effect were found.
To maintain good fertilization ability of semen during cryopreservation is necessary to increase the spermatozoa resistance against cold shock using addition of correct concentration of LDL into the commercially used diluents AndroMed and Bioxcell. Subsequently the fertilization ability of insemination dose is influenced by cooling, the length of equilibration and freezing. The length of equilibration 120 minutes and more as well as gentle way of freezing according to freezing curve, which ensures a gradual decrease of temperature in freezing chamber provided the higher average spermatozoa motility and proportion of live spermatozoa.
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Cow milk quality in relation to milking technology
Forštová, Pavlína ; Stádník, Luděk (advisor)
1. Milk
Properties of Milk
The content of milk
Quality Milk
Change milk quality
Storage of milk
2. Technology milking
Types parlors
herringbone
parallel
tandem
rotary
ring
Lely milking robot
Procedures for milking
3. The quality of milk in relation to technology milking on the farm Dřeveš
- Comparing quality of milk in the classic machine milking, milking one robot (large load) and two robots (normal load) Lely
Classical machine milking 120 pieces
One robot 75 pieces milked, the rest of the dwell
Two robots 110 dairy units
4. Compare the results from the farm to the national average
Comparisons will take place after the two-year intervals.
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Effect of dairy cows housing technology on mastitis occurrence
Brzáková, Lenka ; Stádník, Luděk (advisor) ; Jan, Jan (referee)
The objective of my bachelor thesis was evaluation of quality of housing, milking and making analysis of mastitis on chosen farm.
First part of the thesis contains of literature summary about housing technologies, mastitis issues, their detection, originators, therapy and factors affects against their incidence. Thereafter is this part focused on tipes of milking parlours, corect milking procedures and mechanized milking.
Materials and methodes of the thesis contains the characteristics of chosen farm where the quality and technology of housing, milking and nutrition of milk cows and other factors affecting presence of mastitis such as taking care of cloven hooves was watched in 2014. The incidence, detection and curation of mastitis was watched afterwards. Montamilk s.r.o. farm cradles 1,200 pieces of cattle and milk cows are about 419 out of it.
In the part of thesis named results was watched and evaluated inspection of efficiency in inspecting year 2013 - 2014, which was stated on first lactated cows and on cows which are on second and further lactation. Milk yield was far better with cows on second or futher lactation according to data gathered during the study and expressed by diagram. Diagram of milk components (fat and proteins), which was divided by lactation, was expressed in this part too. Other examinated parameters was occurrence of mastitis - which milk cows, in which season is the occurrence more often, treatment and cost of treatment for one cow. At the end the number of somatic cells for 2014 was represented in diagram for particular lactations.
In part of thesis called discusion the comparison was drowed between results from chosen farm and national average of efficiency checks. From results is obvious that milk cow in Mnotamilk has been better in first lactated cows by 427 kg and in second and more lactated cows by 602 kg than is the avarage in Czech republic.
Far more the milk components in Montamilk was compared with rest of Czech republic and the results was quite similar. The volume of fat of Montamilk cows was lower in every lactation in comparison with milk cows from Czech. Value of fat in Czech republic avarage first lactated cows is 3,87 % in Montamilk 3,85 % and the value of fat on second and more lactation cows is 3,86 % in Czech average and 3,81 % in Montamilk.
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Characteristics important for ensuring of sustainable and development of dairy cattle herds breeding
Lelek, Jaroslav ; Stádník, Luděk (advisor) ; Petr, Petr (referee)
The aim of this dissertation was to assess the influence of characteristics important for
ensuring of sustainable and development of dairy cattle herds breeding. The data investigated
describe approximately 5138 dairy cows in period of four years in selected farming´s of dairy
cattle in district of Jičín. There were these indicators of reproduction processed: conception
rate after first insemination, conception rate after all inseminations, interval of insemination,
index of insemination, service period and meantime; the data were collected from seven
stables of Bohemian Spotted cattle and from seven stables of Holstein cattle. The longevity
was investigated from the number of dairy cows at respective lactation of both investigated
breeds and from the average lactation in respective years. The results of investigation prove
that the average percentage representation of dairy cow at respective lactation between
2012-2015 ranged 33,4 % at first lactation, and 24,68 % at second lactation, and 18,13 %
at third lactation, and 11,48 % at fourth lactation, and 6,53 % at fifth lactation, and 3,32 %
at sixth lactation, and 2,36 % at seventh and more lactations.
The evident data from comparison prove, that till third lactation there is higher percentage
representation of Holstein cattle and from third lactation there are more cows of Bohemian
Spotted cattle, and also the average reached lactation confirms this the value of 2,75 lactation
for Bohemian Spotted cattle and the value of 2,3 lactation for Holstein cattle. The influence of
breed on the average length of lactation was quite weak. The results of rating indicators of
reproduction also have not proven any statistically important difference between both types of
cattle as concerns values reached by each cattle, but the original Bohemian Spotted cattle
reached better results in all indicators, and thus it is probably more suitable for the area of
district Jičín.
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Effect of equilibration and freezing parameters on bull sperm motility after thawing
Beránková, Monika ; Stádník, Luděk (advisor) ; Zuzana, Zuzana (referee)
The hypothesis assume the existence differences in bulls spermatozoa viability after thawing, characterized by higher values of motility and higher proportion of live spermatozoa, in case of prolonged equilibration and lower temperature freezing gradient. The aim of this thesis was to find the influence of different equilibration and freezing curve conditions to on spermatozoa viability after thawing. The theoretical part of the thesis summarizes available information about the bulls semen parameters, methods for its evaluation and especially the processing and long-term preservation.
Within the case study part of the thesis, during the years 2013-2015 the semen of the pre-selected group of 5 breeding bulls was being collected at the Sires insemination center. The group was uniform as to age, breed and housing and treatment conditions. After initial assessment the collected semen was divided into parts, which were then processed in different wals. A part of straws was processed by standard protocol, the other part was processed using different length of equilibration and freezing curve.
First part of the straws was equilibrated in a standard protocol for 120 minutes; subsequently half of the equilibrated straws was standardly frozen by the Direct Freezing method based on 3-phase freezing curve while the other half was frozen using the different 2-phase freezing curve. Second part of the straws was initially equilibrated for 240 minutes with the subsequent freezing, similarly as in the previous case, used 3-phase and 2-phase freezing curve. Frozen straws were then stored in liquid nitrogen container at -196 °C.
Spermatozoa motility was evaluated using CASA. The semen was further evaluated on the proportion of live and dead spermatozoa, using coloration of Eosin and Nigrosine, and on the sensitivity of the spermatozoa membranes, using HOS test, which detects the reaction of spermatozoa tail membrane to hypoosmotic conditions.
The obtained results indicated higher progressive spermatozoa motility (+5.57 %) and higher percentage of live spermatozoa (+4.47 %) following the application of the prolonged equilibration (240 min). When applying the length of equilibration 240 minutes, the values of total spermatozoa motility and HOS test were higher as well, though the differences were not statistically significant. When evaluate the effect of using different freezing curves on the parameters of the spermatozoa after thawing, higher values (average + 1.6 %) at almost all of these parameters using 3-phase freezing curve were found, the diferrences were not statistically significant.
Statistically significant differences among the bulls (P < 0.01) were found in total as well as progressive spermatozoa motility, in the proportion of living spermatozoa and in value of the HOS test - the individuality among the bulls is therefore evident. While evaluating the characteristics of spermatozoa movement by CASA, statistically significant differences among the bulls were confirmed at VCL, VSL, VAP, LIN, STR, WOB (P < 0.01), also between the diferrent length of equilibration (P < 0.01), but were not statistically significant when using diferrent freezing curves.
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Effect of algae feeding on selected qualitative indicators of milk of small ruminants
Novotná, Klára ; Stádník, Luděk (advisor)
Milk fat is one of the most important components of goat milk. Another benefit of goat milk fat is its better digestibility in comparison with cow milk, which is caused by the smaller size of lipid micelles. Goat milk fat contains more lower fatty acids (caproic, caprylic and capric), which affect the production and processing of milk and cause specific flavor of goat's milk and dairy products. In recent years, the subject of many studies the possibility of increasing the content of other health beneficial fatty acids in goat milk, such as conjugated linoleic acid (CLA) and omega-3 and omega-6 polyunsaturated fatty acids. Animal nutrition is a significant factor how to achieve these changes in the fatty acid profile of milk fat. For these purposes can be used as additives for certain species of algae which are the source of nutritionally valuable lipids with a high content of polyene fatty acids, especially omega-3 and omega-6 fatty acids. The ability to change the composition of goat mikl fat, represents an opportunity for the development of new products. Functional foods, such as milk and dairy products enriched with omega-3 and omega-6 fatty acids. The aim of this work is to determine what effect the addition of selected algae (Chlorella vulgaris and Japanochytrium sp.) On milk production and representation of the components of milk, focusing on the composition of milk fat and fatty acid profile in goat milk. Attention was paid to reduction of saturated and increase the proportion of nutritionally beneficial unsaturated fatty acids, in particular n-3 polyunsaturated fatty acids.
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Towards a better understanding of ungulate diets: a methodological approach
Holá, Michaela ; Červený, Jaroslav (advisor) ; Stádník, Luděk (referee)
Populations of European ungulates have grown substantially over recent decades, resulting in considerable environmental and socio-economic impacts. Availability and quality of natural and supplemental food sources are among the main factors driving their population dynamics. Detailed knowledge of feeding strategies of management-targeted species is therefore of primary importance for their successful management. Over time, methods to study the feeding strategies of animals have also evolved considerably but each has its advantages as well as limitations. This doctoral thesis uses a combination of traditional methods (i.e. stomach content analysis) and novel methods (i.e. stable isotope analysis, near infrared reflectance spectroscopy) to achieve a better understanding of feeding strategies of two important ungulate species (i.e. wild boar and red deer) in the Czech Republic, where their populations are on the rise and supplementary feeding is rampant. Next, this dissertation aims to introduce and to establish a basis for these novel methodological approaches for the study of free-ranging individuals. In this respect, it also addresses methodological issues related to their application in this field.
The diet composition of wild boar was investigated by examining stomach contents in order to identify their dependence on food resources of human origin (i.e. agricultural crops and supplemental foods). Foods of human origin were the dominant diet type and constituted the bulk of the diet of wild boar throughout the year. A necessary prerequisite for application of stable isotope analysis is the trophic discrimination factor (i.e. systematic difference between the isotopic composition of the consumer tissues and that of the diet), which was experimentally determined for stable carbon and nitrogen isotopes, the two most commonly used in ecology, in hair tissue of wild boar and red deer. Furthermore, possible sources of variation (such as sex, age, body weight, and lactation) on isotopic discrimination were investigated. The results of the experiments provide a starting point for the successful use of stable isotope analysis in field studies on wild boar and red deer. Finally, application of near infrared reflectance spectroscopy was proven to be useful in measuring faecal indices of food quality for red deer. The results of this thesis will contribute towards efficient management of wild boar and red deer in the Czech Republic.
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Evaluation of cow fertility on profitability of milk production
Čítková, Dana ; Stádník, Luděk (advisor) ; Dalibor, Dalibor (referee)
Objectives of this study were to evaluate effects of milk production level and calving interval, resp. days open period length on the lactation curve parameters and milk production profitability. Data were obtained from Holstein dairy farm. From our results can be stated, that cows with the longest days open period length reached significantly the highest lactation persistence. Due to their ability to maintain high milk productivity after peak day, average daily milk yield was not in these cows, despite long lactation, lower than in cows with shorter lactations. From the economic point of view, milk production profitability with calving interval length decreased and with milk production level increased. Low profitability in cows with long days open period caused high replacement and fertility costs. Feeding costs despite expectations didn´t differ between these groups. All early lactating cows are fed with the same expensive concentrated feeding ration, thats why there is not possible to save feeding costs in high persistent cows with low milk production increase after calving
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