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Study of transport mechanisms of xenobiotics
Richtrová, Monika ; Trejtnar, František (advisor) ; Melicharová, Ludmila (referee)
Aim: Graduation theses was aimed on the use of the isolated rat renal cells to study of transport mechanisms of the selected receptor - specific peptides from the group of somatostatin analogues labeled with convenient radionuclides - 111In-DOTA-octreotate, 125IDOTA- octreotate. Experiment with the selected model substances - sucrose and α-methyl glucoside as markers of active and passive transport was executed. Accumulation process of α-methyl glucoside was tested in renal and pancreatic cells. Further, accumulation of the radiopeptides was researched in presence of the potential inhibitors, which could reduce to retention these radiopeptides and thus also clinically undesirable renal radiotoxic insult. Methods: Isolated rat renal cells were prepared by collagenase technique. Pancreatic rat exocrine cells were prepared from cell line tumor cells. Viability of cells was tested with trypane blue. The accumulation rate of model substances was compared with the accumulation radiopeptides rate: 111In-DOTA-octreotate, 125I-DOTA-octreotate. Transport character was also monitored for low temperatures. Megalin/cubilin membran transport system was researched via their ligands (e.g. albumin, gentamicin), which could inhibit accumulation of the radiopeptides by competition in transport. Results: Cells...
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Study of Fluorescence of Selected Benzimidazoles
Richtrová, Monika ; Kubíček, Vladimír (advisor) ; Lázníčková, Alice (referee)
The influence of used solvents on fluorescence of seven selected benzimidazoles (reduced flubendazole, albendazole, 2-aminobenzimidazole, carbendazim, benomyl, thiabendazole a oxibendazole) is studied in this thesis. It was found, that solvents, used in liquid chromatography as mobile phases, influence the fluorescence of individual benzimidazoles in a very diverse ways. The shape of the fluorescence spectra is very strongly dependent on the structure of a benzimidazole. The obtained results can serve as starting points during a development of chromatographic methods for a benzmidaloles determination with the fluorimetric detection. In the other part of the thesis, a derivatization of hydrolyzed mebendazole with 7- chloro-4-nitrobenzo-2-oxa-1,3-diazole is studied as a way enabling a fluorescence detection of this substance. Unfortunately, the obtained results are not promising.
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Professional morality of teachers in kindergarten
Richtrová, Monika ; Havlová, Jana (advisor) ; Voda, Jan (referee)
BIBLIOGRAPHY IDENTIFICATION Title of thesis: Professional kindergarten teacher morale Department: Department of Primary Education Faculty of Education, Charles University Name and surname: Monika Richtrová Field of Study: Pre-school education Leader: PaeDr. Jana Havlova Year of defense: 2012 ANNOTATION Diploma work "Professional ethics teacher in kindergarten" deals with the professionalism and the moral aspect of and personality of the teacher. In the theoretical part I try, as fully to describe the essence of morality, characterize and define terms related to morality. The research analyzes the professional morality of teachers and a comprehensive look at the possibility of legalization of the moral code of ethics teaching. Part of work is also questionnaire and interview, which relates to the issue, determined educators chomutov region. Key words: ethics, morality, personality, educator, professional ethics, the ethos ofprofessional code of ethics.
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Professional morality of teachers in kindergarten
Richtrová, Monika ; Havlová, Jana (advisor) ; Voda, Jan (referee)
BIBLIOGRAPHY IDENTIFICATION Title of thesis: Professional kindergarten teacher morale Department: Department of Primary Education Faculty of Education, Charles University Name and surname: Monika Richtrová Field of Study: Pre-school education Leader: PaeDr. Jana Havlova Year of defense: 2012 ANNOTATION Diploma work "Professional ethics teacher in kindergarten" deals with the professionalism and the moral aspect of and personality of the teacher. In the theoretical part I try, as fully to describe the essence of morality, characterize and define terms related to morality. The research analyzes the professional morality of teachers and a comprehensive look at the possibility of legalization of the moral code of ethics teaching. Part of work is also questionnaire and interview, which relates to the issue, determined educators chomutov region. Key words: ethics, morality, personality, educator, professional ethics, the ethos ofprofessional code of ethics.
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Study of transport mechanisms of xenobiotics
Richtrová, Monika ; Trejtnar, František (advisor) ; Melicharová, Ludmila (referee)
Aim: Graduation theses was aimed on the use of the isolated rat renal cells to study of transport mechanisms of the selected receptor - specific peptides from the group of somatostatin analogues labeled with convenient radionuclides - 111In-DOTA-octreotate, 125IDOTA- octreotate. Experiment with the selected model substances - sucrose and α-methyl glucoside as markers of active and passive transport was executed. Accumulation process of α-methyl glucoside was tested in renal and pancreatic cells. Further, accumulation of the radiopeptides was researched in presence of the potential inhibitors, which could reduce to retention these radiopeptides and thus also clinically undesirable renal radiotoxic insult. Methods: Isolated rat renal cells were prepared by collagenase technique. Pancreatic rat exocrine cells were prepared from cell line tumor cells. Viability of cells was tested with trypane blue. The accumulation rate of model substances was compared with the accumulation radiopeptides rate: 111In-DOTA-octreotate, 125I-DOTA-octreotate. Transport character was also monitored for low temperatures. Megalin/cubilin membran transport system was researched via their ligands (e.g. albumin, gentamicin), which could inhibit accumulation of the radiopeptides by competition in transport. Results: Cells...
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Study of Fluorescence of Selected Benzimidazoles
Richtrová, Monika ; Lázníčková, Alice (referee) ; Kubíček, Vladimír (advisor)
The influence of used solvents on fluorescence of seven selected benzimidazoles (reduced flubendazole, albendazole, 2-aminobenzimidazole, carbendazim, benomyl, thiabendazole a oxibendazole) is studied in this thesis. It was found, that solvents, used in liquid chromatography as mobile phases, influence the fluorescence of individual benzimidazoles in a very diverse ways. The shape of the fluorescence spectra is very strongly dependent on the structure of a benzimidazole. The obtained results can serve as starting points during a development of chromatographic methods for a benzmidaloles determination with the fluorimetric detection. In the other part of the thesis, a derivatization of hydrolyzed mebendazole with 7- chloro-4-nitrobenzo-2-oxa-1,3-diazole is studied as a way enabling a fluorescence detection of this substance. Unfortunately, the obtained results are not promising.
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