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Fluorescence Spectroscopy: Advanced methods and their defined applications in protein science
Pospíšil, Petr ; Hof, Martin (advisor) ; Heřman, Petr (referee) ; Polívka, Tomáš (referee)
The hydration and dynamics of the biomolecules appear to be vital for their proper biological functioning. In the presented thesis, various fluorescence techniques were developed and applied to access these properties and their changes upon the mutual interactions of the biomolecules. Initially, the solvent relaxation method based on recording time-dependent fluorescence shift (TDFS) was used to map DNA interactions with proteins and lipids by the newly synthesised fluorene dye covalently bound to the DNA. Secondly, copper-transporting ATPase was probed by Badan attached to the copper-binding cysteine-proline-cysteine motif. The variations in hydration were found to be crucial for the proper ATPase function. Third, a detailed study on quenching of Badan/Prodan fluorescence by tryptophan revealed the limitations of the TDFS method for protein studies, which is essential finding for further applications of TDFS. Fourth application involves investigations of heavy atom effects on the excited state relaxation processes by up-conversion approach in iodinated metallocorroles, which are promising dyes for biological imaging. The obtained findings shall help in further tuning of the optical properties of the corroles desired for the variety of applications. Finally, fluorescence correlation spectroscopy...
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Limiting Factors of the European Union Counter-Terrorism Mechanisms
Pospíšil, Petr ; Weiss, Tomáš (advisor) ; Šlosarčík, Ivo (referee)
The basis, upon which this thesis has been built, are the two following trends: occurence of terrorist attacks in the EU and creation of new counter-terrorism institutions on the EU level. A frequency of both trends has been rising: the occurence of terrorist attacks on the EU territory has increased despite the closer cooperation in security and counter-terrorism area that can manifested by adopting new measures and designing new institutions. Therefore, the author asks a question: What factors are hindering a better functioning of the EU counter-terrorism mechanisms? The six most significant EU counter-terrorism institutions with exclusively or overwhelmingly counter-terrorism mandate is subjected to analysis. The respective limits of their functioning have been subsumed to a classification that was created by the author using the general institutional theory, in particular the theory of regimes. The attention is given to an interplay between the EU counter-terrorism bodies and their counterparts on the national level. In case of every institution that has been explored, the author evaluates the following criteria. Firstly, presence of a "patterned behaviour" in sense of cooperation between the national counter-terrorism bodies and the EU institutions. Secondly, a subjective perception of such...
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Managing Users as a Source of Risk
Pospíšil, Petr ; Konečný, Martin (referee) ; Sedlák, Petr (advisor)
This diploma thesis focuses on human resources mainly in Critical information infrastructure and Important information systems. Thesis focuses on the most frequent threats for users and design possible model of threat reduction. Integral part of results is designing of effective security awareness education program according to the Law on Cyber Security.
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Self-assembly and excited energy transfer in bacteriochlorophyll aggregates
Pospíšil, Petr ; Pšenčík, Jakub (advisor) ; Vácha, František (referee)
Bacteriochlorophyll c (BChl c) belongs among photosynthetic pigments. It is found in lightharvesting complexes of green photosynthetic bacteria in the form of self-assembled aggregates. It is possible to prepare BChl c aggregates also in vitro in aqueous solutions, in the presence of certain non-polar molecules (e.g. lipids or carotenoids). In this work, artificial light-harvesting antenna was prepared composed of BChl c and selected azulene derivative. The aggregation of BChl c with azulene enhances the absorption in the near UV region compared to BChl c. Fluorescence spectroscopy was used to study an efficiency of energy transfer from azulene to BChl c. The procedure used was based on fluorencence excitation spectra measurement, and a comparison with absorption 1-T spectra. The measurements were complicated by anomalous fluorencence of azulene, which occurs from the S2 state. Furthermore, the reference diode in the fluorescence spectrometer did not correct properly the signal in the near UV region, where azulene absorbs. The problem was partially solved by preparing the new correction files using selected laser dyes. Excitation spectra demonstrate the presence of the energy transfer from azulene to BChl c, however, it was not yet possible to satisfactorily quantify its efficiency.
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Color-to-Grayscale Conversions
Pospíšil, Petr ; Brejcha, Jan (referee) ; Čadík, Martin (advisor)
Grayscale pictures were never replaced by color ones and will probably never be, because they are indispensable in many areas. In this thesis was chosen three methods for color-to-gray conversion. These methods was implemented afterwards. Within this thesis was also made test set of 24 HDR pictures, which was together with existing set of 24 LDR pictures used to test and evaluate features of implemented methods. These methods were also compared from the point of accurancy and preference using questionnaire.
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Design and Optimization of the Trading Strategy in Financial Markets
Pospíšil, Petr ; Slatinský, Miroslav (referee) ; Budík, Jan (advisor)
This bachelor's thesis is concerned with the design and optimization of a trading strategy on Foreign Exchange – particularly on EURUSD currency pair. The strategy makes use of standard indicators used in technical analysis and is consequently optimized and backtested using history data. The thesis addresses both the development of such a strategy and the main pitfalls, ones with which I have came in contact with and had to solve, are discussed.
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