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Vision for Kotovo
Podešvová, Klára ; Navrátil, Václav (referee) ; Marek, Jiří (advisor)
The service building Kotovo, built between 1970s and 1980s of the previous century, is situated to the south from the city center of Vsetin, in the city part Rokytnice. The building stands at the beginning of the housing estate tight-close to tower blocks. Two floored object in a U form consists of three standalone blocks, that is A, B and C part. Nowadays, Kotovo is used for a variety of services and markets, such as grocery shops, dentist office, post office or children’s library. The city of Vsetin is fully aware of the state of Kotovo and plans to reconstruct the building. First part of the diploma thesis analyses specifics of this space, deals with connecting the object to its surroundings, defines its possible functioning utilization, and above all it finds meaningful vision for the future reconstructions. The goal of the diploma thesis is to tie in to the areas draft potential, make and architectural concept for the whole area and then design one of the buildings. The thesis should work as a vision and the foundation document for the future reconstruction.
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Enzyme activity of recombinant enzymes. Influence of histidine tag.
Navrátil, Václav ; Wsól, Vladimír (advisor) ; Zemanová, Lucie (referee)
Charles University in Prague Faculty of Pharmacy in Hradec Králové Department of Biochemical Sciences Candidate: Václav Navrátil Supervisor: Prof. Ing. Vladimír Wsól, Ph.D. Title of diploma thesis: Enzymatic activity of recombinant enzymes. Evaluation of His- tag influence. AKR1B1, AKR1C1 and AKR1C3 are three representatives from 15 human enzymes of AKR superfamily, so far described. In the body they take part in a number of biochemical pathways and their substrates include both xenobiotics and endogenous compounds. Reduction of glucose to sorbitol catalyzed by AKR1B1 is associated with the development of secondary diabetic complications. AKR1B1 is also involved in cardiotoxic effect of the cytostatic drug daunorubicine. AKR1C1 is physiologically responsible for the conversion of progesterone to less efficient 20α- hydroxyprogesterone, thereby regulates progestational activity. Overexpression of AKR1C1 may be connected with resistance to certain cytostatic agents. AKR1C3 in human catalyzes a reaction in which testosterone and 5α-DHT is formed and it is also associated with development of several cancer types. Significant involvement of these enzymes in a number of pathological processes led to their closer study. The aim of this work was to evaluate influence of His-tag and chosen buffer on the...
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Increasing the range of a vehicle with alternative propulsion
Dryml, Tomáš ; Ranuša, Matúš (referee) ; Navrátil, Václav (advisor)
The bachelor's thesis deals with increasing the range of a vehicle with an alternative drive, which is compressed air. First, an analysis of the pneumatic circuit is performed, from which the areas to be modified result. To improve, it is chosen to find a suitable working pressure and the correct filling, which is calculated on the basis of constant pressure filling and subsequent adiabatic gas expansion. From the theoretical calculation, the runs per cylinder stroke at different pressures are determined and the selected pressures are subsequently experimentally verified. The results show that the most suitable setting increased the range from 331 meters using the original setting, to 616 meters with the new setting.
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Lubricants for bicycle chains
Brychta, Ondřej ; Navrátil, Václav (referee) ; Svoboda, Petr (advisor)
This bachelor thesis deals with the lubrication of bicycle chains. The introduction describes the basics of the friction processes, then there is an analysis of the chains used on bicycles, used materials, analysis of forces and wear in the chains. The last part is devoted to the properties of basic types of lubricants, their additives and the possibilities of their use in cycling.
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Contactless sealing element
Bryksí, Petr ; Navrátil, Václav (referee) ; Omasta, Milan (advisor)
This bachelor's thesis deals with the design of a labyrinth seal element for a tram gearbox. The element should create the most effective seal in the smallest possible space. The work contains a research part dealing with the possibilities of shaft sealing and applicable 3D printing technology. The second part deals with the analysis of the current solution of tram gearbox seals. The construction part deals with the design of the most suitable material and shape to create the greatest resistance, against the ingress of dirt into the gearbox. The result of the work is a model ready for 3D printing and subsequent application, for testing in operating conditions.
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Development of analytical tools for quantification and screening for inhibitors of glutamate carboxypeptidases II and III
Navrátil, Václav ; Konvalinka, Jan (advisor) ; Obšil, Tomáš (referee) ; Šedo, Aleksi (referee)
Glutamate carboxypeptidase II (GCPII) usually called prostate specific membrane antigen (PSMA) is membrane bound metallopeptidase expressed mainly in prostate carcinoma (PCa). Agents targeting GCPII suitable for both imaging and treatment of PCa are in development and they show promising results in advanced clinical trials. Some studies showed that GCPII may serve also as PCa blood serum marker, but this has not been validated due to the lack of methods suitable for accurate detection of GCPII in human blood. Moreover, GCPII is also expressed in brain, where it cleaves inhibitory N-acetyl-α-L- aspartyl-L-glutamate (NAAG) to release excitatory L-glutamate and GCPII inhibition has been shown to be neuroprotective in animal models of several neuropathies. Tight binding inhibitors of GCPII have been identified by rational design, but all have poor bioavailability and thus cannot be used in clinics. Identifying new scaffolds by 'brute force' screening methods is thus essential; however, no such method for GCPII has been developed so far. Glutamate carboxypeptidase III (GCPIII) is also expressed in brain and cleaves NAAG. It is thus an important protein for understanding of GCPII function as well as GCPII targeting in medicine. Here, we focused on development of novel methods for quantification of both...
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TOWNHOUSE ON THE WATERFRONT IN PŘEROV
Superatová, Alena ; Navrátil, Václav (referee) ; Havliš, Karel (advisor)
Subject of this thesis is an urban and architectural design of buildings vacant lot at the corner intersection of streets Brabansko - A small splint near the city center of Přerov. Apartment Building Front Street closes and completes residential block. Corner is cantilevered, giving rise to leeward before. There are shops, facilities of the apartment building and garage. Above the shops are perched three floors forward of mass housing with 15 apartments. The analysis of the city has shown that in Prerov is not a retirement home and no day care. So I decided to tackle this problem. The rear wing of the house serves as a single health center. It has its own entrance, its core communications and access from the garage. Its components except surgeries and rehabilitation is the seat of nursing services. Its main role would be serving the elders who live in their homes. Nurses would be goung out for them, helping with housework, taking clothes to laundry, etc. These care services are already operating in other cities and enjoying great popularity. Residential house is inside the health center connected. Given the proximity care services are particularly suitable housing for the elders. Therefore, the flats designed to lay with disabilities. All apartments are with 2 bedrooms for one or two people. Each dwelling unit has a spacious balcony overlooking the waterfront.
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