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Purification of recombinant proteins by affinity chromatography
Zemek, Ondřej ; Mácha, Jaroslav (advisor) ; Hrdý, Ivan (referee)
The isolation and purification of recombinant proteins is essential for further study of their structural and functional properties. The affinity chromatography is usually the method of choice for this task. In this paper the most used affinity tags are reviewed for their properties and experience with their application. The tags covered here include CBP, MBP, GST, polyhistidine and polyarginine tags, FLAG-tag, Strep-tag II and SpA. The origin and properties of the tags, their influence on form and localization of fusion protein as well as binding, elution and removal are discussed. Keywords: affinity chromatography, recombinant protein, purification, affinity tag
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Vliv počtu návštěv dojnic v systému robotizovaného dojení na parametry mléčné užitkovosti
Mácha, Josef
The aim of the thesis was to clarify the influence of the number of visits of dairy cows in the robotic milking systems on milk yield parameters. An annual observation was carried out for this purpose (November 2017 to October 2018). A total of 729 dairy cows (cases) were evaluated. Among the parameters of milk yield were mainly daily milk yield (kg of milk), number of lactation, lactation phase (days in milk). Furthermore, the influence of temperature in the stable (° C) on the frequency of dairy cows' visits in the robotized milking system was evaluated. There was a highly statisticaly significant effect of the number of successful dairy cow visits in milking robot on the lactation phase, daily milk yieldand fat and protein content of milk (p <0.01). It was found, that with the increasing frequency of dairy cow visits during the day (n = 1 to n = 5), the average daily milk yield of these cows increases (from 18.3 to 48.4 kg of milk), while the fat content and protein decreased (from 4.53 to 3.84%, from 3.59 to 3.40% respectively). Furthermore, it was found that dairy cows with a lower lactation phase had a higher frequency of visits (from 189 days to 25.8 days). Further, a certain trend was observed where higher frequency of visits was found in older cows and lower frequency of visits in young cows (p> 0.05). Furthermore, it was found that the air temperature in the stable did not affect both the overall visit rate of the milking robot and the number of successful or unsuccessful visits (p> 0.05).
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