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Strategic Brand Development
Kopecká, Irina ; Mráček, Pavel (referee) ; Zich, Robert (advisor)
The diploma thesis focuses on the strategic development of the brand of the selected winery Znovín Znojmo. The work is divided into three parts. The theoretical part defines the market, customers, competitors, brand, brand identity and communication mix. The second part of the work is the analytical part, which aims to evaluate the current situation of the company and determine possible steps to develop the brand. The last part of the diploma thesis is a recommendation based on knowledge gained from the first parts for the strategic development of the brand.
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Adsorption of organic compounds onto activated carbon in water treatment process
Kopecká, Ivana
The dissertation thesis focuses on the application of activated carbon for the removal of low molecular weight algal organic matter (AOM) produced by phytoplankton during drinking water treatment, as well as on the effect of AOM on adsorption of anthropogenic micropollutants contained in raw water. The results of this study have been published in international peer-reviewed journals in 4 papers and in 2 conference contributions. The efficiency of AOM removal was studied in laboratory equilibrium and kinetic experiments using different types of granular activated carbon and cellular peptides with molecular weight < 10 kDa produced by cyanobacterium Microcystis aeruginosa. It has been previously confirmed that these peptides are removed with difficulty during the conventional water treatment based on coagulation/flocculation processes and therefore, other methods need to be applied for their restriction. The effect of solution properties on peptide adsorption was assessed by the tests at different pH values and at variable ionic strengths. The negative impact of peptides on the adsorption of organic micropollutants present in raw water was simulated using competitive adsorption experiments with herbicides alachlor and...
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Oxidation of compounds produced by cyanobacteria Microcystis aeruginosa and its impact on water treatment by coagulation
Štědrá, Karolína ; Načeradská, Jana (advisor) ; Kopecká, Ivana (referee)
Due to eutrophication of aquatic environment, massive growth of algae and cyanobacteria occurs and the seasonal algal bloom is formed. Drinking water treatment plants have to deal with the increased amount of cells as well as organic compounds produced by algae and cyanobacteria (so-called AOM). Moreover, the presence of AOM causes a reduction in the efficiency of coagulation, which is an essential process in drinking water treatment. To increase coagulation efficiency, supportive methods, such as ozone oxidation, are added usually prior to coagulation. While many studies focused on the ozonation of algal and cyanobacterial cells, little is known about the effect of ozonation on AOM. This diploma thesis focused on the evaluation of the effect of ozonation in combination with coagulation on the removal of cellular organic compounds (COM) produced by cyanobacteria Microcystis aeruginosa. The effect of ozonation before or after coagulation on COM removal was investigated. The degradation of microcystins, which are naturally contained in the COM sample, was also monitored. Various ozone doses (1 - 10 mg/L) were tested at three pH values - 5, 7 and 9. Two coagulants were utilized - aluminium sulphate and ferric sulphate. Ozonation removed COM negligibly. However, eliminated microcystins, the degradation...
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Chromatographic evaluation of amiodarone and its active metabolite
Kopecká, Iva ; Pilařová, Pavla (advisor) ; Kastner, Petr (referee)
77 Abstract Charles University in Prague, Faculty of Pharmacy in Hradec Králové Department: Pharmaceutical Chemistry and Pharmaceutical Analysis Candidate: Mgr. Iva Kopecká Tutor: PharmDr. Pavla Pilařová, Ph.D. Name of Degree Paper: Chromatographic evaluation of amiodaronu and its active metabolite The main purpose of this thesis was to optimize the existing HPLC method used in University Hospital Hradec Králové for determination of amiodarone and its active metabolite in biological material. For this HPLC method was used Waters Symetry C18 column, 150 x 4,6 mm, 5 µm, mobile phase: acetonitrile: 25 mM phosphate buffer (55:45), flow rate 1,4 ml/min, at 45 ř C, injection 5 µl, UV detection at 242 nm. This method was subsequently transferred to a UHPLC using Phenomenex Kinetex C18 column, 100 x 2,1 mm, 1,7 µm, mobile phase: acetonitrile: 25 mM phosphate buffer (55:45), flow rate 0,3 ml/min, at 45 ř C, injection 5 µl, UV detection at 242 nm. This UHPLC method was modified for eventual detection by mass detector using same UHPLC column, mobile phase: acetonitrile: 0,1% formic acid (55:45), flow rate 0,3 ml/min, at 45 ř C, injection 5 µl, UV detection at 242 nm. At the same time plasma, resp. serum sample isolation was optimized. The best result was achieved by adding 4 µl of 10% zinc sulfate to the plasma sample...
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Applied Mineralogy in Forensic Science field
Kotrlý, Marek ; Matějka, Dobroslav (advisor) ; Kopecká, Ivana (referee) ; Vojtíšek, Pavel (referee)
1 ABSTRACT Surprisingly, applied mineralogy plays an important role in the forensic science field. It is the miner- alogical phases which exist in many kinds of materials and traces but also the mineralogical analysis procedures which are often used in the field of so called trace evidence. For purposes of this study, 4 important fields of forensic microanalysis were chosen. These are those fields in which applied min- eralogy is applied and which characterize the width and heterogeneity of the concerned area. Powder X-ray microdiffraction plays quite an important and irreplaceable role not only in the direct phase analysis of substances in mixtures. The author designed, tested and introduced a set for centra- tion and direct check of the analysed surface during micro-diffraction analysis and also experimentally optimised sensing parameters for the different system configuration. A possibility to calculate the size of the nanoparticles (more precisely the size of a mono crystal domain) was tested. XRD methods ap- plication allows refining organic analysis for example in the case of new synthetic drugs. Micro diffrac- tion was also used for the first complex phase analysis of colour layers of the altar in the chapel in the castle of Křivoklát (dated 1480 - 1490). Forensic analysis of soil phases used to be,...
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Adsorption of low molecular weight algal organic matter onto activated carbon during water treatment
Fialová, Kateřina ; Pivokonský, Martin (advisor) ; Kopecká, Ivana (referee)
This diploma thesis deals with the study of adsorption of low-molecular components of organic substances produced by phytoplankton - AOM (Algal Organic Matter) on AC (Activated Carbon) during drinking water treatment. For the experimental purpose in this thesis, there were used selected amino acids as low molecular substances of AOM that are difficult to remove by conventional water treatment process by coagulation. As adsorbent, there was used a detailed characterized of granulated activated carbon (GAC) - Filtrasorb TL 830 (FTL830) which is intended directly for the purpose of water treatment. There were realizing the equilibrium batch adsorption experiments with three different model amino acids - arginine (Arg), phenylalanine (Phe) and aspartic acid (Asp). There was investigated the efficiency of removing amino acid depending on the solution temperature and pH. Results of the adsorption experiments have shown that the temperature affects the adsorption efficiency. Adsorption is essentially described as an exothermic process but the adsorption of Arg and Phe from an aqueous solution to GAC occurs more efficiently at higher temperatures. It means that the adsorption is the endothermic process. In the case of Arg adsorption, the temperature was found to influence adsorption efficiency less than...
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Monitoring and promoting the health of people with mental disability in the project Healthy Communities
KOPECKÁ, Iveta
The bachelor thesis deals with the creation and verification of a three-month interventional movement program for people with mental disability. The theoretical part of the thesis focuses on the characteristics of people with mental disability, health promotion and familiarization with the Healthy Communities project. The practical part deals with the measured data of the input and output measurements and the effect of the intervention program on the experimental and control group. The aim of the interventional movement program was to meet or approach the research assumptions. In the input measurement BMI 28.33 and in the output 27.67 were measured in the mean values. The spine flexibility was in the entry measurement averaging -12 cm and improved to -9 cm in the output measurement. In the sit-up test, they reached an average of 10 sit-up/ min in the input measurement and improved to 15 sit-up/ min in the output. After processing all measured data, the results showed that the interventional movement program had a positive effect on the probands of the experimental group.
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Application of stable isotope techniques for verifying the geographical origin of endangered plants and animals subject to international trade
Kopecká, Ivana ; Jandová, Kateřina (advisor) ; Horká, Petra (referee)
Elements in nature can exist in several versions, which differ in the amount of neutrons in the nucleus. These modifications are called isotopes. Neutrons have a neutral charge, but do not have a zero weight, therefore isotopes of a single element are differ in weight. Thanks to the sensitive analytical methods, we can discern that the isotope in the sample is contained in what quantity (mass spectrometry is used, from which to retrieve the ratio of stable isotopes in a sample). This can be used in many fields, such as in geology, food ecology, or discovery of geographic origin. The aim of the thesis is in the published literature to determine the extent to which the method of analysis of stable isotopes usable to verify the geographical origin of endangered species of flora and fauna subject to international trade, especially turtles of the genus Testudo. Turtles of the genus Testudo are protected by CITES treaty, which limits, enables and disables trading in endangered species so as to avoid their extinction caused overexploitation for commercial purpose. However, there is still an illegal trade that the supervising authorities are trying to detect and combat. Key words: traceability, provenance determination, authentication, CITES treaty
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Analysis of pigments and binders in colour layer of art works
Kučková, Štěpánka ; Pacáková, Věra (advisor) ; Štulík, Karel (referee) ; Kopecká, Ivana (referee) ; Novotná, Miroslava (referee)
4. Conclusions 1. The method of gas chromatography was successfully optimised for a reliabie oeteňation of individual components in coiophony and Mexioan coPai' ificant 2. of arl the stu<iied pigments, verdigris exhibits the most stgn effect on the composition of fresh corophony and Mexican copal' 3. The degradative processes caused by aftif,rcial ageing of diterpenoids (pimaradiene tolttul"' and abietadiene acids) in colophony and triterpenoids (ct- and B-amyrin) in Mexican copal were detected' ___________ i I -. l i3 c:oriern oť u]tramícrotom. ,,.^'::..:. distonion can be solved by the use oť compare tt," int.u.i A more convenienl reÍlection ...nn,o,..o spectra ", ;;;": J:ffi: ";J-",,;,J.5. The detection iimit corour rayers ,.. Jt ".t infrared spectroscopy for the non-aged moder (*zw) forprussian :',t'' n (w/w) for c6pp1 rimits were rouna Tand0'3-1 "@;;;;: phthalocvanine' 1-3 %o phtharocyanin " r, n'l'.the artinciatl;' ;JTil:t:Jffi:: ó. MALDI.T.F MS is w/w) and indigo (3.l0 % wlw). in fresh and artificial able to ídentify indigo ar are 0.01 o/o lv/wÍ.b. i".: aged model cil;, copper phthalocyanine 7. MALDI-T.. ,, ,o,tt*o and 0'03 n *,* ^i,""' The detection limits their morecurar ions. :ntifies carmine, tu..ui. ropper phthalocyanine' associatedproreins. In addition' t"*;;:1""::"::ff:;;'Jl 8. The technique...
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