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Proteomic characterization of the host cell after interaction with intracellular pathogen Francisella tularensis
Římalová, Pavla ; Šimůnek, Tomáš (advisor) ; Klimentová, Jana (referee)
Diploma Thesis Proteomic characterization of host cell after interaction with intracellular pathogen Francisella tularensis Pavla Římalová Charles University in Prague, Faculty of Pharmacy in Hradec Králové Department of Biochemical Sciences 2009 Francisella tularensis (F. tularensis) is an intracellular gramnegative bacterium, causative agent of the serious human disease tularemia. It is a typical bacterium with unknown mechanisms of pathogenesis. A proteome characterization of host-pathogen interaction could significantly contribute to their elucidation. The aim of this diploma thesis was to reveal the protein expression changes in macrophage mouse cell line J774.2 after infection with F. tularensis using the quantitative "shotgun" proteome technology. The proteomes of host cells infected with F. tularensis for three various time intervals (6, 12 and 24 hours) were compared with the proteome of non-infected cells. Four subcellular fractions were prepared using ProteoExtract Subcellular Extraction Kit (S-PEK, Calbiochem). Fraction enriched for cytosolic proteins was further analyzed and quantified by iTRAQ-HPLC-MS/MS technology. This approach led to the successful identification of 248 proteins, the production of 37 proteins was then observed to be different compared to the non-infected cells....
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Anion receptors based on symmetrically tetrasubstituted calix[4]arenes
Klimentová, Jana
The submitted Ph.D. thesis concerns anion receptors based on symmetrically tetrasubstituted cone calix[4]arenes. These receptors are easily synthesized and display sufficient water solubility to function as receptors for tetrahedral oxoanions. The thesis starts with a brief survey of literature on calix[4]arene anion receptors and sensors. The theoretical part of the thesis deals with a stereochemical investigation of calix[4]arenes from the Cambridge Structural Database (CSD) with emphasis on the influence of inter- and/or intramolecular interactions on the geometry and rigidity of the calix[4]arene scaffold. In order to describe the geometry of the calix[4]arene moiety, it was necessary to introduce new stereochemical parameters , and . The utility of these parameters in evaluating the geometry of the calix[4]arene base frame was confirmed. The stereochemical investigation formed the basis for design of new potential anion receptors based on symmetrically tetrasubstituted cone calix[4]arenes. The experimental part of this work started with synthesis of symmetrically tetrasubstituted calix[4]arene derivatives containing 1-propoxy groups at the lower rim and synthesis of calix[4]arene derivatives with carboxymethoxy groups at the lower rim and enhanced water solubility. Identity and purity of...
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Anion receptors based on symmetrically tetrasubstituted calix[4]arenes
Klimentová, Jana
The submitted Ph.D. thesis concerns anion receptors based on symmetrically tetrasubstituted cone calix[4]arenes. These receptors are easily synthesized and display sufficient water solubility to function as receptors for tetrahedral oxoanions. The thesis starts with a brief survey of literature on calix[4]arene anion receptors and sensors. The theoretical part of the thesis deals with a stereochemical investigation of calix[4]arenes from the Cambridge Structural Database (CSD) with emphasis on the influence of inter- and/or intramolecular interactions on the geometry and rigidity of the calix[4]arene scaffold. In order to describe the geometry of the calix[4]arene moiety, it was necessary to introduce new stereochemical parameters , and . The utility of these parameters in evaluating the geometry of the calix[4]arene base frame was confirmed. The stereochemical investigation formed the basis for design of new potential anion receptors based on symmetrically tetrasubstituted cone calix[4]arenes. The experimental part of this work started with synthesis of symmetrically tetrasubstituted calix[4]arene derivatives containing 1-propoxy groups at the lower rim and synthesis of calix[4]arene derivatives with carboxymethoxy groups at the lower rim and enhanced water solubility. Identity and purity of...
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Anion receptors based on symmetrically tetrasubstituted calix[4]arenes
Klimentová, Jana ; Lukeš, Ivan (advisor) ; Budka, Jan (referee) ; Trávníček, Zdeněk (referee)
The submitted Ph.D. thesis concerns anion receptors based on symmetrically tetrasubstituted cone calixf4]arenes. These receptors are easily synthesized and display sufficient water solubility to function as receptors for tetrahedral oxoanions. The thesis starts with a brief survey of literature on calix[4larene anion receptors and sensors. The theoretical part of the thesis deals with a stereochemical investigation of calixf4]arenes from the Cambridge Structural Database (CSD) with emphasis on the influence of inter- and/or intramolecular interactions on the geometry and rigidity of the calix[4]arene scaffold. In order to describe the geometry of the calixf4]arene moiety, it was necessary to introduce new stereochemical parameters cr, B and 5. The utility of these parameters in evaluating the geometry of the calixf4]arene base frame was confirmed. The stereochemical investigation formed the basis for design of new potential anion receptors based on symmetric al ly tetrasub stituted c o n e calixl4 ] arene s. The experimental part of this work started with synthesis of symmetrically tetrasubstituted calixf4]arene derivatives containing 1-propoxy groups at the lower rim and synthesis of calixf4]arene derivatives with carboxymethoxy groups at the lower rim and enhanced water solubility. Identity and...
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Proteomic characterization of the host cell after interaction with intracellular pathogen Francisella tularensis
Římalová, Pavla ; Klimentová, Jana (referee) ; Šimůnek, Tomáš (advisor)
Diploma Thesis Proteomic characterization of host cell after interaction with intracellular pathogen Francisella tularensis Pavla Římalová Charles University in Prague, Faculty of Pharmacy in Hradec Králové Department of Biochemical Sciences 2009 Francisella tularensis (F. tularensis) is an intracellular gramnegative bacterium, causative agent of the serious human disease tularemia. It is a typical bacterium with unknown mechanisms of pathogenesis. A proteome characterization of host-pathogen interaction could significantly contribute to their elucidation. The aim of this diploma thesis was to reveal the protein expression changes in macrophage mouse cell line J774.2 after infection with F. tularensis using the quantitative "shotgun" proteome technology. The proteomes of host cells infected with F. tularensis for three various time intervals (6, 12 and 24 hours) were compared with the proteome of non-infected cells. Four subcellular fractions were prepared using ProteoExtract Subcellular Extraction Kit (S-PEK, Calbiochem). Fraction enriched for cytosolic proteins was further analyzed and quantified by iTRAQ-HPLC-MS/MS technology. This approach led to the successful identification of 248 proteins, the production of 37 proteins was then observed to be different compared to the non-infected cells....
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Structure modifications of transkarbams - synthesis and evaluation of physico-chemical properties and biological activity focused on mechanism of biological activity
Klimentová, Jana ; Hrabálek, Alexandr (advisor) ; Csöllei, Jozef (referee) ; Nobilis, Milan (referee)
One of the subjects studied in the Department of Inorganic and Organic Chemistry of the Faculty of Pharmacy focuses on the synthesis and biological evaluation of potential transdermal permeation enhancers. Over the past few years, series of esters of 6-aminohexanoic acid of high enhancing activity and their analogues have been prepared. Presently, the structure-activity relationships are being studied. This work contributes to this problem by searching for the mechanism of action of one of the most active compounds, transkarbam 12 (T12). Transdermal permeation enhancers facilitate drug absorption through the skin. Generally, their mechanisms of action include the disturbance of highly ordered structure of stratum corneum, promotion of drug solubility in the vehicle or enhancing the partitioning of drug from the vehicle into stratum corneum. Nevertheless, the exact mechanism is usually unclear. T12 is a carbamic acid salt derived from two molecules of 6-aminohexanoic acid dodecyl ester. In slightly acidic environment (as in stratum corneum, its target place) it decomposes easily releasing a molecule of CO2 and free amino ester. To find out whether this ability contributes to its high activity, a series of T12 analogues with CO2 covalently bound in the polar head was prepared (esters of carbonic,...
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Relationship of young people towards brands
Klimentová, Jana ; Filipová, Alena (advisor) ; Zeman, Jiří (referee)
The goal of my diploma thesis is to describe and evaluate relationship of young people in Czech Republic towards brands. In first chapter I describe historical development of marketing approach towards brands, the function of a brand, its elements and the differences between brands and products. I mention and compare two diverse concepts of brand management - Kellers CBBE concept and Roberts Lovemarks concept. In the second chapter I characterize young Czechs at the age between 15 and 30. I do so thanks to information gathered from different sociological studies and from papers about phenomenom of generation Y. Last chapter follows the previous two and is purely about relationship of young pepople towards brands. I used for it facts described in the thesis above and data that I get from particular qualitative research as well as quantitative research that I realised by myself.
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Business protocol and professional etiquette in Germany
Klimentová, Jana ; Gullová, Soňa (advisor) ; Laschoberová, Libuše (referee)
My thesis describes business protocol and professional etiquette in Germany. In first chapter there are definitions of the words etiquette, social behaviour, business protocol and professional etiquette. You can find there the explanations of the relationship among these words too. In second chapter I describe intercultural differences betweek Czechs and Germans and how these diversity influences their behaviour in business and in other areas. Both of the nations are compared with the method of cultural dimensions made by G.Hofstede and with the method of cultural standards. In next part you can find specific information about business protocol in Germany. I describe the rules we should keep by getting to know each other, by communication, rules we should keep by dressing into a work, behaviour rules connected with business meetings, social evenings and etiquette of gifts. Last chapter characterizes professional etiquette in Germany. It explains behaviour rules at the workplace, by official trips. It describes work etiquette and netiquette.
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