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Research of protective immunity on patients after transplantation.
Pelák, Ondřej ; Kalina, Tomáš (advisor) ; Drda Morávková, Alena (referee)
Depletion of immune functions in patients after hematopoetic stem cell transplantation (HSCT) results in higher frekvencies of several types of oportunistic infections. Especially the reactivation of human cytomegalovirus (CMV) may cause life-threating infections and require antiviral treatment. Within this diploma thesis we compare the function signatures of CD4+ and CD8+ T-cells stimulated by CMV antigens, which were obtained from 63 pacients after HSCT. Polychromatic flow cytometry measurements of CD154 (CD40L), intracellular cytokines (INF-γ and IL-2) and marker of degranulation (CD107a) allowed us to determine the function status of various T-cells simultaneously. We have discovered, that dual production of INF-γ and IL-2 on CD8+ T-cells was present only in pacients controling their CMV reactivations, but was absent in noncontrolers. Single production of INF-γ on CD8+ T-cells was the most abundat subtype, but they most probably represent non-protective memory cells. We have further created two test of NK-cells functionality, one that aims on the detection of their degranulation ability and second, that detects cytotoxic capability of NK-cells. Key words: transplantation, hematopoetic stem cells, T-lymfocytes, immune system recovery, cytomegalovirus, INF-γ, IL-2
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B- and T- lymphocyte subpopulations in lymphocyte-associated immunodeficiencies
Šinkorová, Vendula ; Kalina, Tomáš (advisor) ; Javorková, Eliška (referee)
The antigen-specific immunity consists of cells called T and B lymphocytes. These cells together with cells of non-specific (innate) immunity begin their development in fetal liver and later in bone marrow from the common progenitor, the hematopoietic stem cell. Both B and T lymphocyte lineages then undergo differentiation which is regulated by many cytokines and transcriptional factors and leads to very heterogeneous cohort of subsets. Because the immune system is not only protecting the organism from infections and malignant growth but also from itself, lymphocyte differentiation must pass many checkpoints where B and T clones are strictly selected. Cells of both lineages closely communicate with each other and also with cells of innate immunity. If, due to mutation of protein encoding genes, disturbance of differentiation or malfunction of effector activities providing some of these functions occurs, an immune system malfunction called immunodeficiency arises. Multiparametric immunophenotyping followed by flow cytometry examination has been proven one of the most suitable techniques for studying lymphocyte subsets and lymphocyte- associated immunodeficiencies. Here we describe examples of primary lymphocyte- associated immunodeficiencies, how they affect individual lymphocyte subsets, what it...
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Genetic, humoral and cellular factors of development of autoimmune diseases
Růžičková, Šárka ; Černá, Marie (advisor) ; Kalina, Tomáš (referee) ; Drda Morávková, Alena (referee)
Autoimmune diseases currently represent the most serious medical issues, mainly due to generally increasing number of patients with these diseases. Their pathogenesis is likely caused by hereditary factors, cellular and humoral interactions influenced by external environmental factors, whose knowledge is important both for diagnostics and therapy, and for theoretical immunology. The aim was to examine the correlation between genetic alterations and the production of autoantibodies, clinical manifestation of the disease; to identify joint autoantigens, further to demonstrate development of leukemic cells from originally autoreactive B cells, the role of B lymphocytes in the pathogenesis of the disease and finally to develop a method for detection of B cells recognizing a defined autoantigen. Several predisposing polymorphisms were revealed using genetic analysis however, they were not exclusively associated either to clinical forms of the disease or usable as prediction markers. In addition, the frequency of alleles IL-1RN * 2 and PD3.1 showed ethno- geographical differences and a critical role of size of sample cohorts in assessing of significance of particular polymorphism was demonstrated in GWA studies. The combination of examination of anti-CCP and IgM RF was found as the best and most...
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Analysis of password generators
Chirkova, Mayya ; Pavlíček, Luboš (advisor) ; Kalina, Tomáš (referee)
This bachelor's thesis occupies with comparison of random and memorable passwords. The work has two parts. In the first section of the thesis is presented the problems of the passwords using. The second (practical) part is analyzing the available passwords generators. As the main contribution of the thesis I see the familiarizing users with issues of memorable passwords and the way password's creating.
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Technical solutions of payments for wireless network access
Kučera, Matěj ; Kosek, Jiří (advisor) ; Kalina, Tomáš (referee)
Topic of this bachelor thesis is paid WiFi networks and their technical payment solution. The first chapter deals with the various payment methods that can be used for connection to a paid WiFi network. There are described advantages and disadvantages of different payment methods, problems in their implementation. The second chapter describes several ready-made solution that can be purchased as a complex solution for paid WiFi network. There are compared their features, functionality and price. In the third part, which is the practical part, there is described design and implementation of the payment procedure in Hotel WiFi network, operated by the Network Department of the University of Economics, Prague, at all accommodation and recreational facilities of this organization, in order to give the reader a complex demonstration of how the implementation could look like. This part describes the situation before creating the Hotel network, the basic requirements for this network, its design and the final solution from a technical perspective and from a perspective of a paying customer.
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QoS in a computer network of VŠE
Kalina, Tomáš ; Pavlíček, Luboš (advisor) ; Beneš, Jiří (referee)
The diploma thesis is focused on analysis and deployment of Quality of Services (QoS) in a computer network of The University of Economics in Prague (VŠE). The thesis describes the main QoS technologies focusing on technology Diffserv and its deployment with using available network devices in computer network of VŠE. In thesis the needs of network services and protocols for quality of services with division into different classes of services according to priority are discussed. The thesis explains the benefits and drawbacks of deploying QoS in the environment of VŠE with regard to wired and wireless network. In the practical part recommendations are applied to the production network and subsequently the benefits of modification are evaluated.
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Network access control
Kalina, Tomáš ; Pavlíček, Luboš (advisor) ; Knapovský, Miroslav (referee)
This bachelor's work discusses IT issues of network access control. The first part deals with the theoretical background of network access control. There are explained various ways of authenticating and authorization users. Thanks these function, it is possible to prevent unauthorized persons access to computer networks. Knowledge of these technologies is essential for understanding the next part of the work. The remaining part of the work is concerned with recommendations and draft network access control for various model situations, especially for the college of University of Economics, Prague. Specific examples are given for network infrastructure based on device of the 3com company.
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