|
|
Immunomodulatory mechanisms of stem cells and their use for therapy of ocular disorders
Heřmánková, Barbora
Stem cell-based therapy represents a perspective approach for the treatment of many so far incurable diseases. Mesenchymal stem cells (MSC) are currently the most studied stem cells. They are able to differentiate into different cell types, to produce growth and trophic factors and can suppress the functions of cells of the immune system. During the study of the immunomodulatory properties of MSC, we focused on their effect on B cells. The mechanism of impact of interferon-γ (IFN-γ) on MSC and their effect on the production of interleukin 10 (IL-10) by B cells was analysed. We have demonstrated that MSC-treated with IFN-γ inhibit production of IL-10 by activated B cells via the cyclooxygenase-2 involving pathway. Due to their regenerative and immunomodulatory properties, MSC can be for treatment of many diseases. In this study we focused on the disease and damage of the eye. The limbal stem cells (LSC) are used for the treatment of damaged ocular surface, however their isolation is difficult and they can not be used in all cases of damage. Appropriate candidates in these cases are MSC. Therefore we have decided to compare the therapeutic potential of LSC and MSC isolated from bone marrow or adipose tissue. The study have shown that MSC isolated from bone marrow have a similar regenerative effect on...
|
|
|
Case Study of Physiotherapeutic Treatment of a Patient with the Reverse Total Endoprosthesis of the Shoulder Joint Diagnoses.
Vachová, Anna ; Heřmánková, Barbora (advisor) ; Čejková, Klára (referee)
Title: Case Study of Physiotherapeutic Treatment of a Patient with the Reverse Total Endoprosthesis of the Shoulder Joint Diagnosis. Objectives: The aim of this thesis is an introduction and summary of theoretical and practical knowledge about reverse shoulder replacement and a case study of patient with with the diagnosis. Methods: This bachelor thesis has been devided into two main parts, the general part and the special part. The general part includes theoretical knowledge related to the topic of reverse shoulder replacement and the therapeutic methods used in physiotherapy. The special part includes a case report of the patient with reverse total endoprosthesis of the shoulder joint. This part contains kinesiological examination, diagnostic and therapeutic management and the final assessment of the physiotherapeutic effect. Key words: Shoulder joint, reverse endoprosthesis, rotator cuff, physiotherapy
|
|
|
Immunomodulatory mechanisms of stem cells and their use for therapy of ocular disorders
Heřmánková, Barbora ; Holáň, Vladimír (advisor) ; Heissigerová, Jarmila (referee) ; Indrová, Marie (referee)
Stem cell-based therapy represents a perspective approach for the treatment of many so far incurable diseases. Mesenchymal stem cells (MSC) are currently the most studied stem cells. They are able to differentiate into different cell types, to produce growth and trophic factors and can suppress the functions of cells of the immune system. During the study of the immunomodulatory properties of MSC, we focused on their effect on B cells. The mechanism of impact of interferon-γ (IFN-γ) on MSC and their effect on the production of interleukin 10 (IL-10) by B cells was analysed. We have demonstrated that MSC-treated with IFN-γ inhibit production of IL-10 by activated B cells via the cyclooxygenase-2 involving pathway. Due to their regenerative and immunomodulatory properties, MSC can be for treatment of many diseases. In this study we focused on the disease and damage of the eye. The limbal stem cells (LSC) are used for the treatment of damaged ocular surface, however their isolation is difficult and they can not be used in all cases of damage. Appropriate candidates in these cases are MSC. Therefore we have decided to compare the therapeutic potential of LSC and MSC isolated from bone marrow or adipose tissue. The study have shown that MSC isolated from bone marrow have a similar regenerative effect on...
|
|
|
Clinical applications of stem cells for the treatment of neurodegenerative diseases of CNS
Jančová, Pavlína ; Kubinová, Šárka (advisor) ; Heřmánková, Barbora (referee)
Stem cells have a huge therapeutic potential due to their ability to differentiate in multiple tissues. They could be used for neurodegenerative diseases treatment, which are typical for loss of specific groups of neurons, progressive course and lack of effective treatment due to their complicated pathophysiology, only therapies for elongation and simplification of patients' life are available. This thesis summarizes results of completed clinical studies and informs about ongoing studies, in which stem cell treatments are used for selected neurodegenerative diseases. Stem cell therapy for multiple sclerosis and amyotrophic lateral sclerosis have a long history, some of the studies has proven therapeutic efficiency of stem cells. We don't know much about effect of stem cell treatment for patients with Alzheimer's and Parkinson's diseases, because first clinical studies were finished recently. But all clinical trials have proven safety of stem cell treatment. Replacement of damaged neurons haven't been reached yet, just protection of remaining neurons by neurotrophic and immunomodulatory factors secreted by stem cells. Keywords: neurodegenerative diseases, stem cells, clinical trials, multiple sclerosis, amyotrophic lateral sclerosis, Alzheimer's disease, Parkinson's disease
|
|
|
Sexual dysfunction and pelvic floor dysfunction in patients with systemic rheumatic diseases
Heřmánková, Barbora ; Špiritović, Maja (advisor) ; Nováková, Tereza (referee)
Title: Sexual Dysfunction and Pelvic Floor Dysfunction in Patients with Systemic Rheumatic Diseases Objectives: To assess sexual functions, quality of life and pelvic floor function in female patients with Systemic Sclerosis (SSc) and Idiopathic Inflammatory Myopathies (IIM) compared to age-/sex-matched healthy controls (HC). Methods: In total, 41 women with SSc (mean age: 50.9, disease duration: 5.8 years), who fulfilled the ACR/EULAR 2013 classification criteria for SSc, 41 healthy controls (mean age: 50.9) without rheumatic diseases, 22 women with IIM [mean age: 55.1, disease duration: 7.9 years, dermatomyositis (DM, 8)/ polymyositis (PM, 10)/ necrotizing myopathy (IMNM, 3)/ inclusion body myositis (IBM, 1)], who fulfilled the Bohan/Peter 1975 diagnostic criteria for DM/PM, and 22 healthy controls (mean age: 55.1 years) filled in 12 well-established and validated questionnaires assessing sexual function/quality of life, pelvic floor function, fatigue, physical activity and depression. Results: Compared to HC, patients with SSc and IIM had significantly higher prevalence and greater severity of sexual dysfunction (FSFI, BISF-W: in all subscales as well as total scores), dysfunction of pelvic floor (PISQ-12), and worse sexual quality of life (SQoL-F). Worse scores in SSc patients were associated...
|
|
|
Protective education in educational institutions in the Czech Republic
Heřmánková, Barbora ; Šotolová, Eva (advisor) ; Mlčková, Marie (referee)
This diploma thesis describes the current state of legal regulation and implementation of protective education in the Czech Republic. The first part contains the theoretical background of the whole work and deals with the terminological apparatus. It also contains psychological aspects of juvenile delinquency and history of criminal responsibility of children and adolescents in the Czech Republic. The second part contains the valid legislation of this particular with an accent on the issue of protective education. The third part describes current practice in institutional and protective education, with particular emphasis on placement and transfer of children and adolescents to individual facilities, which it specifies. Part Four examines the current aplicable rules in realization of protective education within a specific set of facilities and the legal regulations of juvenile delinquency in Slovakia. The fifth part describes the changes brought about by the new system of private law and the current proposals for changing the whole system of institutional education of children and youth. The seventh part contains a research, in which a representative sample of professional public from various types of institutions was approached and the research is aimed at gaining knowledge about the practice of...
|
|
|
Targeted therapy: the promising implication of mesenchymal stem cells
Hrabovská, Lucia ; Krylov, Vladimír (advisor) ; Heřmánková, Barbora (referee)
Mesenchymal stem cells (MSCs), or multipotent stromal cell are non-hematopoetic cells from mesoderm germline. They can be isolated from various types of tissues like bone marrow, adi- pose tissue, umbilical cord blood, chorionic villi, amniotic fluid, placenta and fetal liver or lung. Due to their self-renewal ability, targeted migration and their potential to differentiate, MSCs hold a great potential to be employed in clinical medicine. They have a capacity of differentia- tion into a variety cell lineages like osteocytes, chondrocytes, adipocytes and other cell types. Recovery of damage tissue is taking place in a treatment of fractures, cardiovascular and neu- rological disorders. Furthermore, MSCs exhibit significant affinity for tumor cells, so they can be used as carriers of anti-tumor drugs. In this bachelor thesis I discuss the current knowledge in the field of targeted therapy. Key words: targeted therapy, tumor, mesenchymal stem cells
|
|
|
Study of effect of immunosuppressive drugs on interaction of mesenchymal stem cells with immune cells
Heřmánková, Barbora ; Krulová, Magdaléna (advisor) ; Indrová, Marie (referee)
Mesenchymal stem cells (MSC) represent a heterogenous population of nonhematopoietic stem cells with multipotent differential potential. MSC can be isolated from various tissues of organism, the most common tissue are bone marrow or adipose tissue. MSC are good candidates for treatment of autoimmune diseases and possess the ability to prevent graft rejection or graft versus host disease. The immunosuppressive drugs are currently used for inhibition of unwanted immune reaction but they exhibit serious side effects. The use of MSC in therapy can reduce doses of immunosuppressive drugs and eliminate side effects. The study of MSC and immunosuppressant interactions should be detected before MSC can be used for clinical application. We aimed to analyze the interaction between MSC and immunosuppressive drugs and their effect on immune cells. Cyclosporine A and mycophenolate mofetil were used in our research. We demonstrated changes in the expression of surface molecules, production of interleukin 6 and in metabolic activity of MSC after treatment with immunosuppressive drugs. MSC are in organism, in cooperation with the number of other cells. Therefore we studied MSC cocultured with splenocytes in the presence of immunosuppressive drugs. Our results show the effect of MSC and immunosuppressive drugs on different...
|
|
|
Upper limb assessment in multiple sclerosis
Heřmánková, Barbora ; Řasová, Kamila (advisor) ; Sedláčková, Barbora (referee)
My thesis is focused on the possibilities of examination of upper limb function in patients with multiple sclerosis. It also details the interpretation of outcome measures. The work was part of an international study called Psychometric properties of Outcome Measures of upper limb function in multiple sclerosis which was aimed at creating a systematic review of outcome measures that provide reliable data. Given the high prevalence of functional upper limb dysfunction in patients with multiple sclerosis, their examination quality is a prerequisite for successful rehabilitation. The theoretical part of my work is occupied with the detailed description of each test which were evaluated through study to be relevant. From this perspective it tis really important that outcome measures are reliable, valid and responsive. These properties, called psychometric properties, were mentioned in my thesis.
|
|
|
The influence of Toll-like receptors on immunomodulatory properties of mesenchymal stem cells
Heřmánková, Barbora ; Krulová, Magdaléna (advisor) ; Indrová, Marie (referee)
Immunosuppressive properties of mesenchymal stem cells (MSCs) offer potential tools for cell-based regenerative therapy and immunotherapy. MSCs express a large number of Toll like receptors (TLRs) recognizing multiple pathogen-associated molecular patterns (PAMPs), which could be found in patients microenvironment and modulate activity of MSCs. TLR ligands could lead to modulation of proliferation, differentiation, immunosuppression, migration, polarization and production of cytokine. This work discusses the effect of TLRs on immunomodulatory properties of MSCs, because there are lots of contrasts in the results about their effect.
|
guest ::
RSS feed.