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National Repository of Grey Literature

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	Development and applications of ionization techniques in ambient mass spectrometry Rejšek, Jan ; Vrkoslav, Vladimír ; Cvačka, Josef Ambient mass spectrometry is a sharply developing subject area. Desorption electrospray ionization (DESI) and desorption atmospheric pressure photoionization (DAPPI) are two types of ionization techniques in the ambient mass spectometry. Herein, they were investigated in two research areas: (1) TLC analysis. (2) Localization of insect defense glands. Detailed record
	Sborník 14. ročníku Školy hmotnostní spektrometrie Cvačka, Josef ; Vrkoslav, Vladimír Proceedings of full versions of lectures covering basics of mass spectrometry, ionization techniques, mass analyzers, coupling of mass spectrometry to chromatography, quantification, proteomics, metabolomics and data interpretation. Detailed record
	Mass spectrometry in lipidomics Cvačka, Josef Current approaches of analytical chemistry in lipidomics based on mass spectrometry and chromatography are discussed in detail. Examples of qualitative, quantitative and imaging approaches are given. Detailed record
	Anovel defensin from the mucus of the wood wasp Xiphydria camelus Monincová, Lenka ; Fučík, Vladimír ; Voburka, Zdeněk ; Cvačka, Josef ; Čeřovský, Václav ; Šrůtka, P. We have isolated a novel defensin from the mucus of the wood wasp Xiphydria camelus. The Edman degradation in 50 cycles revealed a peptide sequence similar to other insect defensins. The complete 55 amino acid residues sequence of X. camelus defensin was proposed based on the mass spectrometry analysis and by the deduction based on the comparison with the sequences of other hymenopteran defensins. The defensin was tested against both Gram-positive and -negative bacteria. Detailed record
	Imaging mass spectrometry of cuticular lipids of Drosophila melanogaster Kaftan, Filip ; Vrkoslav, Vladimír ; Cvačka, Josef ; Svatoš, A. The spatial distribution of cuticular lipids on the surface (head, thorax, abdomen and wings) of Drosophila melanogaster flies has been imaged and studied using MALDI-TOF mass spectrometry and scanning electron microscopy. Detailed record
	Methodology of lipid analysis of vernix caseosa using MALDI-TOF MS Míková, Radka ; Vrkoslav, Vladimír ; Doležal, A. ; Plavka, R. ; Cvačka, Josef This project is aimed to lipid analysis of intact molecules and optimization of MALDI-TOF MS method for these lipids. Detailed record
	Analysis of plant membrane lipids by RP-HPLC/HR-ESI-MS Zábranská, Marie ; Vrkoslav, Vladimír ; Cvačka, Josef Monogalactosyldiacylglycerols (MGDGs) and digalactosyldiacylglycerols (DGDGs) are glycolipids forming the major part of biological membranes in photosynthesizing organisms. The individual species differ from fatty acids linked to sn-1 and sn-2 possition on glycerol backbone. Complexity of their mixtures is increased by enzymatic or chemical oxidation of unsaturated species. The aim of this work was to develop and optimize RP-HPLC/HR-ESI-MS2 methods for analysis of oxidized and non-oxidized MGDGs and DGDGs in plant samples. MGDGs and DGDGs were isolated from Arabidopsis thaliana and Melissa officinalis. Detailed record
	Separation and identification of monogalactosyldiacylglycerols and digalactosyldiacylglycerols by RP-HPLC/ESI-MS Krásová, Marie ; Cvačka, Josef RP-HPLC/ESI-MS methods for analyzing complex mixtures of monogalactosyldiacylglycerols (MGDGs) and digalactosyldiacylglycerols (DGDGs) was developed. The methods allow separation and detection of more than 30 molecular species of MGDGs and more than 40 molecular species of DGDGs in plant samples. Detailed record
	New peptides with antimicrobial activity isolated from the venom of the bumblebee Bombus pascuorum (Hymenoptera: Apidae) Ježek, Rudolf ; Janechová, D. ; Cvačka, Josef ; Slaninová, Jiřina ; Voburka, Zdeněk ; Hovorka, Oldřich ; Fučík, Vladimír ; Borovičková, Lenka ; Maloň, Petr ; Čeřovský, Václav Six new heptadecapeptides were isolated and synthesized by standard Fmoc solid phase peptide synthesistogether with several structural analogues. Antimicrobial activities were correlated with the peptides hydrophobicity, hydrophobic moment, alpha-helicity and HPLC retention time. Detailed record
	Hmotnostní spektrometrie peptidů z jedových váčků čmeláků Cvačka, Josef ; Voburka, Zdeněk ; Hovorka, Oldřich ; Nováková, Kateřina ; Valterová, Irena ; Čeřovský, Václav Five novel structurally related peptides were isolated and characterized from the venom of the bumblebee Bombus terrestris. Some of these peptides consisting of 13-18 amino acids residues presented strong antimicrobial activity against Bacillus subtilis. Detailed record

National Repository of Grey Literature : 97 records found   88 - 97  jump to record:

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4	Cvačka, Jan

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