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The role of glycoconjugates in cell signalling - CEACAM1 and Fc ε RI
Dudková, Lenka ; Šmíd, František (advisor) ; Stiborová, Marie (referee) ; Bílková, Zuzana (referee)
Carcinoembryonic cell adhesion molecule 1 (CEACAM1) is human membrane glycoprotein. The purpose of the first part of this thesis was to isolate CEACAM1 glycoprotein from bile and characterise its purity and recovery that have not been described before. Affinity chromatography of CEACAM1 on hydrazide-activated cellulose with immobilized monoclonal anti-CEA F34-187 antibodies is described. The method is based on the oxidation of the immunoglobulin carbohydrate component located on the Fc fragment of antibody by periodate and oriented bounding to hydrazide-activated matrix. Crude protein fraction from bile was applied onto the affinity column and CEACAM1 was eluted with 6 M guanidine-HCl. A single immunopositive 85 kDa band of CEACAM1 was detected on the western blot with anti-CEA antibody. Probably due to the high glycosylation of CEACAM1 any common method of protein staining was not applicable except staining with lectin.
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Study of Pharmaceuticals Degradationby Advanced Oxidation Processes
Bílková, Zuzana ; Čáslavský, Josef (referee) ; Beklová, Miroslava (referee) ; Zachariášová,, Milena (referee) ; Vávrová, Milada (advisor)
At present, the issue of occurrence of female sex hormones, estrogens and progestogens, in aquatic ecosystems is often discussed by experts and the general public. These substances of steroid structure can be difficult to remove completely by conventional wastewater and drinking water treatment technologies. In given context advanced oxidation processes based on in situ generation of highly reactive hydroxyl radicals can be a suitable technique. This thesis deals with the study of kinetics and degradation products of photocatalytic decomposition of seven female sex hormones (estrone, -estradiol, estriol, ethinylestradiol, diethylstilbestrol, progesterone and norethindrone). Experiments were conducted in a laboratory glass reactor, which was equipped with an energy efficient UV-A LED light source (365 nm emission wavelength) and an immobilised photocatalyst in a form of TiO2 five-layer film deposited on glass. Model samples of water with the initial hormone concentration of 1 mg·L-1 were used and the degradation process was monitored by an HPLC-MS method. In the given system all compounds of interest except estriol had very significant tendency to be adsorb. In the case of estriol the formal rate constant of photocatalytic decomposition was determined based on the Langmuir-Hinshelwood model for two different initial concentrations, 0.5527 hour-1 (1 mg·L-1) and 0.1929 hour-1 (5 mg·L-1), and by comparison of these values it was found that the higher degraded compound concentration, the slower decomposition (fivefold increase of the initial concentration resulted in the constant decrease to almost one-third). Moreover nine degradation products of estriol photocatalytic decomposition were recorded and their structure was designed based on mass spectra. In the second thematic part of the thesis attention was paid to development of a SPE-HPLC-MS method for simultaneous determination of female sex hormones in water ecosystems, with emphasis on an extraction part optimization. The final samples treatment process included besides extraction with Supel™ Select HLB 200 mg SPE cartridges also mechanical impurities removal, hormones extraction from solids trapped on filtration material, sample acidification and extract purification with Supelclean™ ENVI-Florisil® cartridges. Optimised method was used for determination of female sex hormones in two Brno rivers, Svitava and Svratka. In the most cases the concentration was below the detection or quantification limit.
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Degradation of pyrethroids using heterogeneous catalysts
Janík, Ondřej ; Bílková, Zuzana (referee) ; Vávrová, Milada (advisor)
This thesis was provided to study fotocatalytic degradation of polutants on the titanium dioxide, as modern eliminating method useful for removing of polutants out of the environmental matrix. There are also studied pyrethroids, as one of the most used pesticides in recent ages, and posibility of degradation of pyrethroids using degradation on titanium dioxide in aquatic system. This study contains an experiment of fotocatalytic degradation of pyrethroids on titanium dioxide in vitro with final analysis using GC-ECD.
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Entity’s transition from single to double-entry accounting according to terms of religious organizations
Bílková, Zuzana ; Zouhar, Tomáš (advisor) ; Poutník, Lukáš (referee)
The bachelor thesis contains information about accounting system that are used in churches located in the Czech Republic. It focuses on the transition from single entry accounting system to double-entry accounting and therefore thesis determines the position of churches in the society by regulatory framework and bookeeping. The aim is to bring the issue of the valuation of fixed assets during the transition and finding such a property that could not be valued in these types of entities. In the practical section there are various methods of valuation of fixed assets during the transition used in practice. Finally, there are listed illustrative examples with different procedure of valuation of fixed assets. After reviewing the materials dealing with this subject and consultations with people working in the field a verdict has been reached that a every long-term asset can be and also must be evaluated during the transition.
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