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Optical amplifiers and their applications
Dvořák, Tomáš ; Havliš, Ondřej (referee) ; Münster, Petr (advisor)
The main purpose of this diploma thesis is to study of possibilities of optical amplification, ways of realization optical amplifiers and construction of operational EDFA amplifier . According to gained information and knowledge about optical amplifiers, the basic configuration of EDFA amplifier with posibility of connecting up to two EDFA modules, which uses erbium-dopped fibers for amplification, was made. There was also developed a 3D model of front control panel of the amplifier and placement of particullar items was planned. Next step was the construction of EDFA amplifier device and measuring of its parameters and features.
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Multigenerational Coexistence / Happy Living in a Castle
Habancová, Anežka ; Dvořák, Tomáš (referee) ; Mléčka, Jan (advisor)
Previously, the bulk of the population lived in rural areas. The whole family lived there together in the family house on the same property. Young helped the elderly and old to their offspring, with households with children, with the economy. With the advent of industrialization and mobilization, many young people moved into the cities, to work, to the newly constructed blocks of residential houses, which were rather less than they could live with a large extended family. Mutual cooperation was suddenly far away. The town has more people, more jobs and more older people, which the state took care of them - homes for the elderly - retirees concentration in one place, without daily contact with young, with family. Thanks to good quality of health care older people is increasing and this problem is must be solved. The country has still semi-detached houses and multigenerational houses, where the family lived with grandparents, but in the city is hard to find to buy or rent two apartments near each other. The main concept of my work is to create a semi-detached flats, flats with common vestibule, which connects one smaller and one larger apartment. Use the castle for this principle is more than appropriate - will be returned the housing for the family and each of generation finds in the castle grounds space for their activities or employment. This area still remains open to inhabitant of the city and offer them new possibilities for leisure (restaurants and an amphitheater).
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Concrete skeleton construction
Dvořák, Tomáš ; Novotný,, Petr (referee) ; Šulák, Pavel (advisor)
The master’s thesis is focused on a design of reinforced concrete prefabricated skeleton of the sports hall. Object of the design is internal traverse frame of the hall which consists of reinforced concrete prestressed truss, columns and concrete pile construction of foundation. Thesis includes working-out of static calculation, elaboration drawings of shape, reinforcement of solved elements and evolvement of assembly drawings. The rest of the project parts are not analyzed.
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Monitoring the success of transfection of cell line 293 HEK
Dvořák, Tomáš ; Španová, Alena (referee) ; Ševčík, Mojmír (advisor)
Diploma thesis is based on monitoring the succes of transfection of cell linie HEK293. In theoretical part are described principles of transfection methods, cell lines, vectors and reporter genes. HEK293 cells EBNA1 were used for practical part. It was studied the difference between GFP and EGFP plasmids. As well as using various transfection reagents under different culture conditions.
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