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The role of C-reactive protein in cardiac ischemic tolerance
Perglerová, Aneta ; Neckář, Jan (advisor) ; Vebr, Pavel (referee)
Ischemic heart disease (CHD) is a set of pathophysiological states, disorders of blood flow and oxygen supply of the myocardium due to vascular constriction or thrombus blockage. Inflammation plays an important role in CHD. The inflammatory response is associated with the synthesis of acute phase proteins in the liver such as C-reactive protein (CRP). CRP plays an important role in acute forms of CHD such as myocardial infarction (MI). The development of CHD may be supported by the occurrence of some of the risk factors (eg. atherosclerosis, hypertension, plasma CRP). Increased CRP levels may support the initiation of atherosclerotic plaque formation as well as in the case of hypertension the presence of CRP increases the risk of developing CHD. The healing proces after acute MI is accompanied by an inflammatory phase where CRP occurs naturally and CRP is important to accelerate inflammation. There may be a situation which inflammation goes into a chronic phase because it is not terminated in time, with constant CRP synthesis. High levels of CRP may decrease the prognosis after MI. The elevated plasma CRP has a negative effect on the expansion of MI and the associated ventricular dilatation, which may result in a rupture of the cardiac wall. Hypertrophy is the compensatory mechanism of the...
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Procalcitonin as an early marker of bacterial infection
Mikulová, Lenka ; Beránek, Martin (advisor) ; Drastíková, Monika (referee)
Introduction: Procalcitonin (PCT) is a 13 kD protein with a chain of 116 amino acids. It consists of parafollicularcells of the thyroid gland. Inflammatory procalcitonin is made up of 114 amino acids. Procalcitonin from plasma in the course of inflammatory process, does not consistof C-cells of the thyroid gland but it is generatedin the liver, and, to a lesser extent, in the lungs, kidneys, testes, prostate and small intestine. Objectives: 1. To compare the levels and the dynamics of C-reactive protein (CRP) and procalcitonin in the inflammatory process. in three patients with different diagnoses. To compare the number of examined PCT according to indicated VFN (General University Hospital) departments in the years 2009, 2012 and 2015. 3. To study the dynamics of the number of examined PCT in the years 2001- 2016. Methods: The levels of CRP and PCT were measured in the ÚLBLD laboratory of the General University Hospital in Prague in the period from May 20to June 17, 2016. The CRP was measured by immunoturbidimetricanalyzerUnicelDxC 880i. The PCT was measured by theCobas analyser using the electrochemiluminescence technology. In order to evaluate the data, graphs were processed with levels of bothanalytesfor 3 patients. Further, data needed for the evaluation of the above mentioned objectives no. 2...
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Significance of determination SAA of comparison with CRP and procalcitonin
Daňková, Michaela ; Zadina, Jiří (advisor) ; Průša, Richard (referee)
The purpose of the bachelor thesis is to evaluate serum amyloid (SAA) as a diagnostic marker in selected patient groups and to compare the results of SAA with those of C-reactive protein (CRP) and procalcitonin (PCT). SAA, CRP and PCT are acute-phase proteins, whose blood concentration significantly increases within a few hours after an inflammatory stimulus. The synthesis, caused by proinflammatory cytokines, takes place in the liver. CRP is the most and longest used acute-phase reactant in routine practice. As indicators of inflammation, SAA and especially procalcitonin have been used more lately. Although SAA is an acute-phase reactant as sensitive as CRP, it is not employed so often in the diagnosis since a method suitable for routine diagnosis was not developed until recently. Briefly describing the acute-phase proteins, the theoretical part elaborates on the characterization and comparison of SAA, CRP and PCT. It further outlines methods for the determination of individual analytes. The experimental part focuses on defining selected patient groups and the immunonephelometric method for measuring SAA by the Immage 800 biochemical analyzer. The data obtained in three selected patient groups have been statistically evaluated. The aim of the thesis has been to evaluate the correlation of inflammatory...
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The role of C-reactive protein in cardiac ischemic tolerance
Perglerová, Aneta ; Neckář, Jan (advisor) ; Vebr, Pavel (referee)
Ischemic heart disease (CHD) is a set of pathophysiological states, disorders of blood flow and oxygen supply of the myocardium due to vascular constriction or thrombus blockage. Inflammation plays an important role in CHD. The inflammatory response is associated with the synthesis of acute phase proteins in the liver such as C-reactive protein (CRP). CRP plays an important role in acute forms of CHD such as myocardial infarction (MI). The development of CHD may be supported by the occurrence of some of the risk factors (eg. atherosclerosis, hypertension, plasma CRP). Increased CRP levels may support the initiation of atherosclerotic plaque formation as well as in the case of hypertension the presence of CRP increases the risk of developing CHD. The healing proces after acute MI is accompanied by an inflammatory phase where CRP occurs naturally and CRP is important to accelerate inflammation. There may be a situation which inflammation goes into a chronic phase because it is not terminated in time, with constant CRP synthesis. High levels of CRP may decrease the prognosis after MI. The elevated plasma CRP has a negative effect on the expansion of MI and the associated ventricular dilatation, which may result in a rupture of the cardiac wall. Hypertrophy is the compensatory mechanism of the...
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Procalcitonin as an early marker of bacterial infection
Mikulová, Lenka ; Beránek, Martin (advisor) ; Drastíková, Monika (referee)
Introduction: Procalcitonin (PCT) is a 13 kD protein with a chain of 116 amino acids. It consists of parafollicularcells of the thyroid gland. Inflammatory procalcitonin is made up of 114 amino acids. Procalcitonin from plasma in the course of inflammatory process, does not consistof C-cells of the thyroid gland but it is generatedin the liver, and, to a lesser extent, in the lungs, kidneys, testes, prostate and small intestine. Objectives: 1. To compare the levels and the dynamics of C-reactive protein (CRP) and procalcitonin in the inflammatory process. in three patients with different diagnoses. To compare the number of examined PCT according to indicated VFN (General University Hospital) departments in the years 2009, 2012 and 2015. 3. To study the dynamics of the number of examined PCT in the years 2001- 2016. Methods: The levels of CRP and PCT were measured in the ÚLBLD laboratory of the General University Hospital in Prague in the period from May 20to June 17, 2016. The CRP was measured by immunoturbidimetricanalyzerUnicelDxC 880i. The PCT was measured by theCobas analyser using the electrochemiluminescence technology. In order to evaluate the data, graphs were processed with levels of bothanalytesfor 3 patients. Further, data needed for the evaluation of the above mentioned objectives no. 2...
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Procalcitonin in critically ill patients
Bureš, Martin ; Průcha, Miroslav (advisor) ; Zazula, Roman (referee)
The aim of this thesis was to determinate the levels of procalcitonin and Creactive protein in the serum of patients who met the criteria of sepsis diagnosis and the comparison with positive blood cultures that is the exact confirmation of infectious etiology of SIRS (Systemic Inflammatory Response Syndrome). Powered by TCPDF (www.tcpdf.org)
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Significance of determination SAA of comparison with CRP and procalcitonin
Daňková, Michaela ; Zadina, Jiří (advisor) ; Průša, Richard (referee)
The purpose of the bachelor thesis is to evaluate serum amyloid (SAA) as a diagnostic marker in selected patient groups and to compare the results of SAA with those of C-reactive protein (CRP) and procalcitonin (PCT). SAA, CRP and PCT are acute-phase proteins, whose blood concentration significantly increases within a few hours after an inflammatory stimulus. The synthesis, caused by proinflammatory cytokines, takes place in the liver. CRP is the most and longest used acute-phase reactant in routine practice. As indicators of inflammation, SAA and especially procalcitonin have been used more lately. Although SAA is an acute-phase reactant as sensitive as CRP, it is not employed so often in the diagnosis since a method suitable for routine diagnosis was not developed until recently. Briefly describing the acute-phase proteins, the theoretical part elaborates on the characterization and comparison of SAA, CRP and PCT. It further outlines methods for the determination of individual analytes. The experimental part focuses on defining selected patient groups and the immunonephelometric method for measuring SAA by the Immage 800 biochemical analyzer. The data obtained in three selected patient groups have been statistically evaluated. The aim of the thesis has been to evaluate the correlation of inflammatory...
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