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Aktivace biouhlu z pšeničných otrub
Sauchanka, Katsiaryna ; Kalina, Michal (oponent) ; Pořízka, Jaromír (vedoucí práce)
Tato diplomová práce je věnována problematice výroby biouhlu z pšeničných otrub a způsobům aktivace jeho povrchu. V teoretické části jsou stručně představeny informace o biouhlu, jeho fyzikálně-chemických vlastnostech, způsobech výroby a možnostech modifikace povrchu. Také je zde prezentována informace o EBC certifikátu, který uvádí požadavky pro biouhlí uvedené na trh. Praktická část je zaměřena na aktivaci povrchu biocharu a porovnání výsledků s dostupnou literaturou a Evropským biouhelným certifikátem.
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Utilization of lignocellulose materials for biotechnological production of polyhydroxyalkanoates
Kučera, Dan ; Márová, Ivana (oponent) ; Obruča, Stanislav (vedoucí práce)
This diploma thesis describes the possibilities of utilization of lignocellulosic material as a renewable resource to produce polyhydroxyalkanoates (PHA) by biotechnological methods. The theoretical part focuses on the characterization of plant waste biomass, its enzymatic saccharification and the possibilities of production and isolation of hydrolytic enzymes. Furthermore, the literature review deals with bacterial PHA production and the possibility to utilize lignocellulosic biomass for their production. In the experimental part, waste substrates hydrolyzed by chemical and enzymatic pathways were selected. Apple, wine and rapeseed pomace and spent coffee grounds were used as waste substrates. Burkholderia cepacia was employed for PHA production from obtained hydrolysates. Apple pomace appears to be the most promising substrate. It turned out that the selected bacterial strain is capable to utilize waste substrates even without previous treatment. The supernatant exhibited following activities after cultivation: protease, lipase (0.47 nmol/(mL•min)), cellulase for CMC (6.05 nmol/(mL•min)) and filter paper (4.63 nmol/(mL•min)) and xylanase (1.71 nmol/(mL•min)). These enzymes can represent an interesting byproduct PHA production from agricultural waste materials. The work assesses also the effect of time course of cultivation and method of hydrolysis on the resulting PHA production and enzymatic activity of industrially interesting enzymes.
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Aktivace biouhlu z pšeničných otrub
Sauchanka, Katsiaryna ; Kalina, Michal (oponent) ; Pořízka, Jaromír (vedoucí práce)
Tato diplomová práce je věnována problematice výroby biouhlu z pšeničných otrub a způsobům aktivace jeho povrchu. V teoretické části jsou stručně představeny informace o biouhlu, jeho fyzikálně-chemických vlastnostech, způsobech výroby a možnostech modifikace povrchu. Také je zde prezentována informace o EBC certifikátu, který uvádí požadavky pro biouhlí uvedené na trh. Praktická část je zaměřena na aktivaci povrchu biocharu a porovnání výsledků s dostupnou literaturou a Evropským biouhelným certifikátem.
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Utilization of lignocellulose materials for biotechnological production of polyhydroxyalkanoates
Kučera, Dan ; Márová, Ivana (oponent) ; Obruča, Stanislav (vedoucí práce)
This diploma thesis describes the possibilities of utilization of lignocellulosic material as a renewable resource to produce polyhydroxyalkanoates (PHA) by biotechnological methods. The theoretical part focuses on the characterization of plant waste biomass, its enzymatic saccharification and the possibilities of production and isolation of hydrolytic enzymes. Furthermore, the literature review deals with bacterial PHA production and the possibility to utilize lignocellulosic biomass for their production. In the experimental part, waste substrates hydrolyzed by chemical and enzymatic pathways were selected. Apple, wine and rapeseed pomace and spent coffee grounds were used as waste substrates. Burkholderia cepacia was employed for PHA production from obtained hydrolysates. Apple pomace appears to be the most promising substrate. It turned out that the selected bacterial strain is capable to utilize waste substrates even without previous treatment. The supernatant exhibited following activities after cultivation: protease, lipase (0.47 nmol/(mL•min)), cellulase for CMC (6.05 nmol/(mL•min)) and filter paper (4.63 nmol/(mL•min)) and xylanase (1.71 nmol/(mL•min)). These enzymes can represent an interesting byproduct PHA production from agricultural waste materials. The work assesses also the effect of time course of cultivation and method of hydrolysis on the resulting PHA production and enzymatic activity of industrially interesting enzymes.
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