|
|
PCR identification of selected bacteria in cheeses
Gregušová, Barbora ; Burdychová, Radka (oponent) ; Španová, Alena (vedoucí práce)
This work is focused on identification and species specification of a collection of 44 clostridial strains using molecular-genetic methods. DNA of bacteria isolated from late-blowing defected cheeses was used. The purified DNA was diluted to 10 ng/µl and its ability to be amplified was verified by PCR with universal primers. By genus-specific PCR was proved that DNA of all samples belongs to Clostridium genus. Based on species-specific PCR reactions, it was determined that 7 strains belong to C. butyricum and 12 strains belong to C. tyrobutyricum. 15 strains were positively detected in both species-specific PCRs and therefore identified as mixed cultures. Another 10 strains were not classified into tested species. Strains with the gene encoding the enzyme hydrogenase hydA were searched using PCR. Specific PCR products for this gene were detected in 30 strains of the analysed collection. Especially intense were the amplicons by all strains belonging to C. butyricum species.
|
|
|
Produkce vodíku s využitím bakterií rodu Clostridium
Filová, Dagmar ; Kvasničková, Eva (oponent) ; Rittich, Bohuslav (vedoucí práce)
Bakalárska práca sa zaoberá produkciou vodíka baktériami rodu Clostridium – konkrétne C. butyricum a C. tyrobutyricum. V teoretickej časti sú rozobrané spôsoby výroby vodíka, rozdelené na biologické a nebiologické metódy. Práca sa ďalej zameriava na praktické využitie vyššie uvedených mikroorganizmov na účely výroby vodíku. Ako jedno z možných rastových médií pre vyššie uvedené baktérie predstavuje srvátku z mliekarenských výrob. V experimentálnej časti práce bola použitá polymerázová reťazová reakcia na identifikáciu baktérií rodu Clostridium, druhov Clostridium butyricum a Clostridium tyrobutyricum. PCR produkty boli z dôvodu overenia špecifity reamplifikované s použitím rovnakých primerov. Ďalej bola pomocou PCR dokázaná prítomnosť génu na produkciu enzýmu hydrogenázy A.
|
|
|
PCR identification of selected bacteria in cheeses
Gregušová, Barbora ; Burdychová, Radka (oponent) ; Španová, Alena (vedoucí práce)
This work is focused on identification and species specification of a collection of 44 clostridial strains using molecular-genetic methods. DNA of bacteria isolated from late-blowing defected cheeses was used. The purified DNA was diluted to 10 ng/µl and its ability to be amplified was verified by PCR with universal primers. By genus-specific PCR was proved that DNA of all samples belongs to Clostridium genus. Based on species-specific PCR reactions, it was determined that 7 strains belong to C. butyricum and 12 strains belong to C. tyrobutyricum. 15 strains were positively detected in both species-specific PCRs and therefore identified as mixed cultures. Another 10 strains were not classified into tested species. Strains with the gene encoding the enzyme hydrogenase hydA were searched using PCR. Specific PCR products for this gene were detected in 30 strains of the analysed collection. Especially intense were the amplicons by all strains belonging to C. butyricum species.
|
|
|
Produkce vodíku s využitím bakterií rodu Clostridium
Filová, Dagmar ; Kvasničková, Eva (oponent) ; Rittich, Bohuslav (vedoucí práce)
Bakalárska práca sa zaoberá produkciou vodíka baktériami rodu Clostridium – konkrétne C. butyricum a C. tyrobutyricum. V teoretickej časti sú rozobrané spôsoby výroby vodíka, rozdelené na biologické a nebiologické metódy. Práca sa ďalej zameriava na praktické využitie vyššie uvedených mikroorganizmov na účely výroby vodíku. Ako jedno z možných rastových médií pre vyššie uvedené baktérie predstavuje srvátku z mliekarenských výrob. V experimentálnej časti práce bola použitá polymerázová reťazová reakcia na identifikáciu baktérií rodu Clostridium, druhov Clostridium butyricum a Clostridium tyrobutyricum. PCR produkty boli z dôvodu overenia špecifity reamplifikované s použitím rovnakých primerov. Ďalej bola pomocou PCR dokázaná prítomnosť génu na produkciu enzýmu hydrogenázy A.
|
host ::
RSS.