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Study of thin film organic materials thickness
Hegerová, Lucie ; Veselý, Michal (referee) ; Zmeškal, Oldřich (advisor)
The diploma thesis deals with the determination of thickness and refractive index of thin organic films using image analysis. In the theoretical part there are described principles of the methods, which are used to prepare the films (spin coating, inkjet printing, vapour deposition), the characteristics of thin films, ways of finding out the thickness and refractive index of substances (weight methods, electric methods, method based on measurement of absorption coefficient of light, interference microscopy, ellipsometry) and also image analysis (harmonic and wavelet analysis). Interference microscope Epival - Interpako (Carl Zeiss Jena), digital camera Nikon Coolpix 5400 and computer were used for the determination of thickness and refractive index. The thicknesses of layers were set on the basis of interference images of edges and grooves – both from the side of the metal contact and the side of underlying glass. The refractive indices of thin layers were then set using the recorded figures. In the final part of the thesis there are discussed the results of interference images photographed along the full length of the aluminium contact which are used for measuring electrical characteristics of DPP structures. The produces are thicknesses and refractive indices of individual layers.
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Using image analysis to determine the number of colonies of microorganisms
Kováčiková, Eva ; Obruča, Stanislav (referee) ; Zmeškal, Oldřich (advisor)
This bachelor thesis focuses on the usage of image analysis in the determination of the quantity and of the size of microbial colonies. Microorganism used in the experiment was Saccharomyces cerevisiae, which forms regular shapes of colonies on the solid medium. The applied methods of image analysis were based on wavelet transform. Fractal analysis of digital images was performed using a computer program HarFA and fractal parameters were defined. The obtained data was used for graph representation of distribution and frequency of colonies.
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Study population of bacteria by image analysis methods
Ševčík, Jan ; Veselá, Mária (referee) ; Zmeškal, Oldřich (advisor)
This bachelor thesis is focused on monitoring and application of image analysis methods on Bacillus megaterium and Cupriavidus necator bacteria. The introduction describes a general knowledge about bacteria, microscopic and image analysis methods. At first it was necessary to prepare suitable microscopic specimens using Gram staining for the observation of a bacteria cell. Every picture of bacteria was taken using a CCD camera and a microscope. Box–Counting method was used to identify the optimal threshold value, corresponding to their fractal measure (or surface areas covered with bacteria) and fractal dimension (or entropy of their arrangement on the surface area). After thresholding, the images were further processed using the Mass–Radius method, which defines a distribution of bacteria relative to the centroid of a cluster.
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Study of thin transparent films properties
Schmiedová, Veronika ; Veselý, Michal (referee) ; Zmeškal, Oldřich (advisor)
The main aim of this bachelor´s thesis is a study of thin transparent layers of organic materials (PPV, P3HT, TiO2, DPP). The theoretical part describe the methods of quality examinations of their surface, mostly the use of optical and interference microscopy. For elaboration of data was used the image analysis via HarFA program. Thickness of layer and the index of refraction was provided by interference microscope. From the interference images was identified the amplitude of groove for various values of treshold, but mainly the interface inhomogenity of groove, which proofs the surface inhomogenity of samples. For the images taken by the optical microscope were allocated the average errors (but also errors of superior sorts) defining the surface inhomogenities of samples. The conlusion of this study compare the results of both methods.
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Study of thin films refractive index by interference microscopy
Procházka, Petr ; Veselý, Michal (referee) ; Zmeškal, Oldřich (advisor)
This bachelor thesis is focused to measure of spectral dependences of refractive index in thin layers. The object of this thesis was measurement of dispersion dependences of refractive index. The theoretical part deals with present methods of refractive index measurements and quantities associated with. These methods were ordered from the easiest to the most complex methods. Intention of own work was reached results by the very easy method of interference mi-croscopy. Thin layers of various organic matters were investigated by optical microscope and main-ly by interference microscope to determine their optical attributes – refractive index and his dis-persion dependences. There were images, which were made by interference microscope and digital camera. These images were subjected to image analysis by computer program HarFA. Dispersion dependences of refractive index were proved by elaboration by this computer pro-gram.
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Structure and Properties of Aluminium Cast Alloys
Samek, Petr ; Podrábský, Tomáš (referee) ; Juliš, Martin (advisor)
The bachelor work is focused on favour of relationship between structure and porperties of aluminium cast alloy AlSi7Mg0,6 in dependence of the effect of cooling rate. The AlSi7Mg0.6 alloy is commonly used in foundry industry to produce high stressed automotive components like motor blocks and cylinder heads. The properties of the material correspond directly to their microstructure which is given by the parameters of the solidification process. Within the frame of experiment were got structural characteristics (DAS, porosity) depending up on method of cooling. There were watched location of structure defects in volume of specimens.
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Klasifikace odrůd hrachu setého pomocí neuronových sítí
Dobešová, Anna
This thesis is focused on classification of pea varieties by means neural networks procedure. Variety testing is an applied branch, which is strictly legally regulated. New methods and techniques are only slowly implemented. One option for an improving of the process of granting plant variety rights is to perform an image analysis of tested plants morphometric features. In the thesis, own solution for the classification of shape characteristics of pea by neural networks is designed and implemented. The analysis is focused on standards of pea. Achieved results are globally unique, because there is no other known application for classification of shape characteristics of plants by image analysis during variety registration. An advantage of the solution is fully automated process of analysis, which is realized in MATLAB computing environment. The neural network was trained by data from fields trials provided by National Plant Variety Office of Central Institute for Supervising and Testing in Agriculture. The solution will be adopted within practical testing procedures.
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Design and implementation of instruments for monitoring and evaluating physical activity of insects
KAKAŠ, Štefan
The aim of the diploma thesis is the development and subsequent construction of the device whose function is measuring and evaluating physical activity of insects. This instrument is unique in the Czech Republic and will be able to record physical activity of insects by using a video camera. Later that it will be able in this video shot back to reconstruct kinematic and statistical data about the movement of insects with software which is developed with Visual Basic .NET. On the base sufficient od obtained data it can be used to verify the premise of biologists about nervous systém, brain function and behavioral of Drosophila Melangostar which is more than a century used as model for genetics experiments. Finally, the conclusion compares the result of previously known and available software solutions in the world of biology, which are still used as a source for the results of performed experiments.
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Generative propagation of selected species of the genus \kur{Rebutia}
HORNÁT, Milan
In most genera of the family Cactaceae, as well as in the genus Rebutia never been specifically studied and measured the influence of abiotic factors, affecting plant development from seed sowing through juvenile stage to their flowering stage. Monitoring temperature, relative humidity and dew point in the greenhouse environment, along with regular photographic documentation of plant growth and subjective observation, was used for design optimization methodology planting selected species of the genus Rebutia. Regular measurements were carried out from March 2010 to January 2011. It was found that the decisive factor influencing the growth of plants, originating from the high mountains of the tropics, the temperature is. It was developed and practically tested method of construction of growth curves using photographs image analysis of growth, also applicable to other taxa.
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Morfocytometric investigation of haemocytes in comparative hematology
ŠUBJAK, David
Standard haematological procedures in preclinical toxicity studies are mostly carried out on mammals, more recent haematotoxicological models represent invertebrates because they can be used in the screening of myelotoxicity. In the present study, we examined haemocytes of Culex pipiens as a new potential alternative haematological in vivo model. Preparations for microscopic analysis were made from the hemolymph stained by Pappenheim's method for overstriking morphology and by toluidin blue and AgNOR for overstriking nucleoles. Microscopic photos were processed by image analysis and size of cells, nuclei and nucleoli were evaluated. Six types of haemocytes were found: granulocytes, prohemocytes, spherulocytes, plasmatocytes, adipohemocytes and oenocytes. Thanks to image analysis we made a morfometric profile of each type of haemocytes and set the nucleolar coefficient. We described significant differences among prohemocytes, plasmocytes and oenocytes size. On the grounds of nucleoles size we divided haemocytes to next two significant groups. Image analysis seems to be very helpful for distinction among various haemocyte lines but we cant use it for generally separation without personal control thanks to presence of statistically significant findings for only some comparisons cell types.
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