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Characteristics of rainfall in observed data and regional climate model simulations
Svoboda, Vojtěch ; Pech, Pavel (advisor) ; Josef, Josef (referee)
Precipitation in the form of heavy rainfall events is of significant societal concern, not only due to the potential for more frequent flash floods, after evidence of changes in rainfall characteristics has recently strengthened. Despite the importance of individual rainfall events with respect to many hydrological applications, only a few studies dealt with characteristics of individual rainfall events (in contrast with the other daily/sub-daily indices of rainfall depths/intensities). Dissertation thesis presents a comprehensive analysis of heavy rainfall event characteristics for the Czech Republic derived from observed data and large ensemble of regional climate model (RCM) simulations. In addition, spatial correlation structure of observed rainfall data at a mesoscale region of north-eastern Bohemia was analysed. Since an RCM grid box represents a spatial average rather than a point measurement, the effects from areal averaging of rainfall data on characteristics of events were investigated considering the observed data. Characteristics of rainfall events were evaluated according to several indices against the at-site and area-averaged observed data for the control period 1981-2000. The changes of rainfall event characteristics were assesed over two scenario periods (2020-2049 and 2070-2099) with respect to control period. We analysed also relations between changes in simulated rainfall event characteristics and changes in radiative forcing and temperature.

Application of mathematical models for simulation of hydrological conditions in selected streams
Kurková, Marie ; Vašků, Zdeněk (advisor) ; Michal, Michal (referee)
Flood is a natural phenomenon that occurs at different intensities and irregular time intervals. As to natural disasters, floods represent the greatest direct threat for the Czech Republic. They may cause serious critical situations during which not only extensive material damages are done, but may bring also losses of the lives of inhabitants in affected areas as well as vast devastation of cultural landscape including environmental damages. Important from the viewpoint of the elimination of potential threats and consequences of such events is the information issued by flood forecasting service about the character and size of flood areas for individual N-year flood discharges and specific flood scenarios. An adequate image of depths and flow rates in the longitudinal or cross profile of the watercourse during a flood event is provided by the hydrodynamic model. This is why the information obtained from the hydrodynamic models occupies a privileged position from the viewpoint of the protection of citizens' lives and mitigation of damage to their property. The first study is situated on the river Úhlava in meadows by Příchovice near the town Přeštice. The proposal of flood-protection measures is contained in Territorial control documentation. The documentation was elaborated on the basis of hydraulic calculations and experiences from the flood in August 2002. The mathematical model is practically used in the study of analysis of proposed flood-protection measures. The analysis is based on mathematical simulation of water outflow and water level on the river Úhlava. It is possible to use the non-commercial software Hec-Ras, version 3.1.1., for the simulation itself. One of the points of view of the possibility of using proposed flood-protection measures is total efficiency. The mathematical model is posssible to use as a basis of support for realization of proposed flood-protection measures on the river Úhlava in meadows by Příchovice within the grant programme "Program prevence před povodněmi II" under the control of the Ministry of Agriculture. In the second case the mathematical model is practically used in the study of hydrotechnical analysis of streams in cadastral unit. The analysis is based on matjematical simulation of water outflow and water level on chosen streams. It is possible to use the noncomercial software HEC-RAS for the own simulation. The analysis should be shown on dangerous places in the interest place. The mathematical model is possible of using to use as basis for revaluation of action in spatial plan or for view of the flood-protection measures in the village Mochtín. Basic input into the hydrodynamic models is represented by altimetry data. One of ways to obtain such data is through the method of aerial laser scanning (ALS) from the digital relief model (DRM). This method is considered one of the most accurate methods for obtaining altimetry data. Its bottleneck is however incapacity of recording terrain geometry under water surface due to the fact that laser beam is absorbed by water mass. The absence of geometric data on watercourse discharge area may perceptibly affect results of modelling, especially if a missing part of the channel represents a significant discharge area with its capacity. One of methods for eliminating the deficiency is a sufficient channel recess by means of software tools such as CroSolver. The third submitted paper deals with the construction of a hydrodynamic model using 5th generation DRM data, and compares outputs from this model at various discharges with a model based on the altimetry data modified by using the CroSolver tool. Outputs from the two hydrodynamic models are compared in HEC-RAS programme with the use of recessed data and with the use of unmodified DRM. The comparison is done on the sections of two watercourses with different terrain morphology and watercourse size. A complementary output is the comparison of inundation areas issuing from both model variants. Our results indicate that differences in the outputs are significant namely in the lower discharges (Q1, Q5) whereas for Q50 and Q100 the difference is negligible with a great role being played by morphology of the modelled area and by the watercourse size.

The analysis of the weather impact on the shape and shift of the production frontier
Hřebíková, Barbora ; Čechura, Lukáš (advisor) ; Peterová, Jarmila (referee)
Although weather is a significant determinant of agriculture production, it is not a common practice in production analysis to investigate on its direct impact on the level of final production. We assume that the problem is methodological, since it is difficult to find a proper proxy variable for weather in these models. Thus, in the common production models, the weather is often included into a set of unmeasured determinants that affects the level of final production and farmers productivity (statistical noise, random error). The aim of this dissertation is to solve this methodological issues and find the way to define weather and its impacts in a form of proxy variable, to include this variable into proper econometric model and to apply the model. The purpose of this dissertation is to get beyond the empirical knowledge and define econometric model that would quantify weather impacts as a part of mutually (un)conditioned factors of final production, to specify the model and apply it. The dissertation is based on the assumption that the method of stochastic frontier analysis (SFA) represents a potential opportunity to treat the weather as a specific (though not firm-controllable) factor of production and technical efficiency. SFA is parametric method based on econometric approach. Its starting point is the stochastic frontier production function. The method was presented in the work of Aigner, Lovell and Schmidt (1977) and Meusen and van den Broeck (1977). Unlike commonly used econometric models, SFA is based on analysis of production frontier that is formed by deterministic production frontier function and the compound error term. The compound error term consists of two parts -- random error (statistical noise, error term) and technical inefficiency. Technical inefficiency represents the difference in the actual level of production of the producer, and the maximum attainable (possible) level that would be achieved if the producer used a particular combination of production factors in a maximum technically efficient way. Over time, it has been developed on a number of aspects - see time variant and invariant inefficiency, heteroscedasticity, measurement and unmeasured heterogeneity. Along with the DEA, SFA has become the preferred methodology in the area of production frontier and productivity and efficiency analysis in agriculture. Lately, it has been applied for example by Bakusc, Fertő and Fogarasi (2008) Mathijs and Swinnen (2001), Hockmann and Pieniadz (2007), Bokusheva and Kumbhakar (2008) Hockmann et al. (2007), Čechura a Hockmann (2011, 2012), and Čechura et al. (2014 a, b). We assume that the weather impacts should be analysed with regard to technical efficiency, rather than as a part of statistical noise. Implementation of weather in part of deterministic production function rather than in the statistical noise is a significant change in the methodical approach within the stochastic frontier analysis. Analysis of the weather impacts on the changes in the level of TE has not been greatly recorded in the associated literature and is, therefore, considered as the main contribution of this work for the current theory of production frontier estimation, or the technological effectiveness, in the field of agriculture. Taking into account other variables that are important for the relationship and whose inclusion would enhance the explanatory power of the model was part of the objective of this work.Thus, the possible effect of heterogeneity was taken into account when models were formulated and final results discussed. The paper first defined and discussed possible ways how to incorporate the effects of the weather into production frontier model. Assessing the possibility of inclusion of weather in these models was based on the theoretical framework for the development of stochastic frontier analysis, which defines the concept of technical efficiency, distance functions theory, stochastic production function theory and the methodology and techniques that are applied within the framework of SFA, which were relevant for the purpose of this work. Then, the weather impacts on the shape and shift of production frontier and technical efficiency of czech cereal production in the years 2004-2011 was analyzed. The analysis was based on the assumption that there are two ways how to define variables representing weather in these models. One way is to use specific climatic data, which directly describe the state of the weather. For the purpose of this thesis, the variables mean air temperature (AVTit) and sum of precipitation (SUMPit) in the period between planting and harvest of cereals in the individual regions of Czech republic (NUTS 3) were selected. Variables were calculated from the data on monthly mean air temperatures and monthly sums of precipitation on the regional levels provided by Czech hydro-meteorological institute CHMI. Another way to define weather variable is to use a proxy variable. In this dissertation, the calculation of climatic index (KITit) was applied. Climatic index was calculated as a sum of ratios between the actual yield levels and approximated yield levels of wheat, barley and rye, weighted by the importance of each plant in a cereal production protfolio in each region of the Czech republic. Yield levels were approximated by the linear trend functions, yield and weights were calculated with the use of data on regional production and sown area under individual grains by year at the level of regional production (NUTS 3) provided by Czech Statistical Office. Both ways of weather definition are associated with some advantages and disadvantages. Particular climatic data are very precise specificatopn of the actual weather conditions, however, to capture their impacts on the level of final production, they must be implemented into model correctly along with the number of other factors, which have an impact on the level of final production. Climatic index, on the other hand, relates the weather impacts directly to the yield levels (it has been based on the assumption that the violation from yield trends are caused by the weather impacts), though, it does not accomodate the concrete weather characteristics. The analysis was applied on unbalanced panel data consisting of the information on the individual production of 803 producers specialized on cereal production, which have each the observations from at least two years out of total 8-years time serie. Specialization on crop production was defined as minimum 50% share of cereal production on the total plant production. Final panel consists of 2332 observations in total. The values of AVTit, SUMPit a KITit has been associated with each individual producer according to his local jurisdiction for a particular region. Weather impacts in the three specified forms were implemented into models that were defined as stochastic production frontier models that capture the possible heterogeneity effects. The aim is to identify the impact of weather on shift and shape of production frontier. Through the defined models, the production technology and technical efficiency were estimated. We assume that the proposed inclusion in weather impacts will lead to a better explanatory power of defined models, as a result of weather extraction from a random components of the model, or from a set of unmeasured factors causing heterogeneity of the sample, respectivelly. Two types of models were applied to estimate TE - Fixed management model (FMM) and Random parameter model (RPM). Models were defined as translogarithmic multiple-output distance function. The analyzed endogene variable is cereal production (expressed in thousands of EUR). Other two outputs, other plant production and animal production (both expressed in thousands of EUR) are expressed as the share on cereal production and they appear on the right side of the equation together with the exogene variables representing production factors labour (in AWU), total utilized land (in acres), capital (sum of contract work, especially machinery work, and depreciation, expressed in thousands of EUR), specific material (represented by the costs of seeds, plants, fertilisers and crop protection, expressed in thousands of EUR), and other material (in thousands of EUR). The values of all three outputs, capital, and material inputs were deflated by the the country price indexes taken from the EUROSTAT database (2005=100). In Random parameter model, heterogeneity is captured in random parameters and in the determinants of distribution of the technical inefficiency, uit. All production factors were defined as a random parameters and weather in form of KITit enters the mean of uit and so it represents the possible source of unmeasured heterogeneity of a sample. In fixed management model, heterogeneity is defined as a special factor representing firm specific effects, mi. This factor represents unmeasured sources of heterogeneity of sample and enters the model in interaction with other production factors and the with the trend variable, tit.Trend variable represents the impact of technological change at a time t for each producer i. The weather impacts in form of variables AVTit a SUMPit is, together with production factors, excluded from the set of firm specific effects and it is also numerically expressed. That way weather becomes a measured source of heterogeneity of a sample. Both types of models were estimated also without the weather impacts specification in order to obtain the benchmark against which the effects of weather impacts specification on production frontier and technical efficiency is evaluated. Easier interpretation of results was achieved by naming all five estimated models as follows: FMM is a name of fixed management model that does not include specified weather variables, AVT is a name for fixed management model including weather impacts in form of average temperatures AVTit, SUMP is name of model which includes weather impacts in form of sum of precipitations SUMPit, RPM is random parameter model that does not account for weather impacts, KIT is random parameter model that includes climatic index KITit into the mean of inefficiency. All estimated models fullfilled the conditions of monotonicity and kvasikonvexity for each production factor with the exception of capital in FMM, AVT, SUMP and RPM model. Violating the kvasikonvexity condition is against the theoretical assumptions the models are based on, however, since capital is also insignificant, it is not necesary to regard model as incorrect specification. Violation of kvasikonvexity condition can be caused by the presence of other factor, which might have contraproductive influence on final production in relation to capital. For example, Cechura and Hockann (2014) mention imperfections of capital market as possible cause of inadequate use of this production factor with respect to technological change. Insufficient significancy of capital can be the result of incorrect specification of variable itself, as capital is defined as investment depreciation and sum of contract work in the whole production process and not only capital related to crop production. The importance of capital in relation to crop production is, thus, not strong enough to be significant. Except of capital are all other production factors significant on the significancy level of 0,01. All estimated models exhibit a common pattern as far as production elasticity is concerned. The highest elasticity is attributed to production factors specific and othe material. Production elasticity of specific material reaches values of 0,29-0,38, the highest in model KIT and lowest of the values in model AVT. Production elasticity of other material reahed even higher values in the range 0,40-0,47. Highest elasticity of othe material was estimated by model AVT and lowest by model KIT. Lowest production elasticity are attributed to production factors labour and land. Labour reached elasticity between 0,006 and 0,129 and land reached production elasticity in the range of 0,114 a 0,129. All estimated models displayed simmilar results regarding production elasticities of production factors, which also correspond with theoretical presumptions about production elasticities -- highest values of elasticity of material inputs correspond with naturally high flexibility of these production factors, while lowest values of elasticity of land corresponds with theoretical aspect of land as relativelly inelastic production factor. Low production elasticity of labour was explained as a result of lower labor intensity of cereals sector compared to other sectors. Production elasticity of weather is significant both in form of average temperatures between planting and harvest in a given region, AVTit, and form of total precipitation between planting and harvest in a given region, SUMPit. Production elasticity of AVTit, reach rather high value of 0,3691, which is in the same level as production elasticities of material inputs. Production elasticity of SUMPit is also significant and reach rather high lower value of 0,1489. Both parameters shows significant impact of weather on the level of final crop production. Sum of production elasticities in all models reach the values around 1, indicating constant returns of scale, RS (RSRPM=1,0064, RSKIT=0,9738, RSSUMP =1,00002, RSFMM= 0,9992, RSAVT=1,0018.). The results correspond with the conclusion of Cechura (2009) and Cechura and Hockmann (2014) about the constant returns of scale in cereals sector in Czech republic. Since the value of RS is calculated only with the use of production elasticities of production factors, almost identical result provided by all three specifications of fixed management model is a proof of correct model specification. Further, the significance of technological change and its impact on final production and production elasticities were reviewed. Technological change, TCH, represents changes in production technology over time through reported period. It is commonly assumed that there is improvement on production technology over time. All estimated models prooved significant impact of TCH on the level of final production. All specified fixed management models indicate positive impaact of TCH, which accelerates over time. Estimated random parameter models gave contradicting results -- model KIT implies that TCH is negative and decelerating in time, while model RPM indicates positive impact of TCH on the level of final production, which is also decelerating in time. It was concluded, that in case that weather is not included into model, it can have a direct impact on the positive direction of TCH effect, which can be captured by implementing weather into model and so the TCH becomes negative. However, as to be discussed later, random parameter model appeared not as a suitable specification for analyzed relationship and so the estimate of the TCH impact might have been distorted. The impact of technological progress on the production elasticities (so-called biased technological change) is in fixed management models displayed by parameters representing the interaction of production factors with trend variable. The hypothesis of time invariant parameters (Hicks neutral technological change) associated with the production factors is rejected for all models except the model AVT. Significant baised technological change is confirmed for models FMM and SUMP. Biased technological change is other material-saving and specific material-intensive. In the AVT model, where weather is represented by average temperatures, AVTit, technological change is not significant in relation to any production factors. In both random parameter models, rejection of hypothesis of time invariant parameters only confirms significance of technological change in relation to final crop production. Nonsignificant effect of technological change on production elasticity of labor, land and capital indicates a generally low ability of farmers to respond to technological developments, which can be explained by two reasons. The first reason can the possible complications in adaptation to the conditions of the EU common agricultural market (eg. there are not created adequate conditions in the domestic market, which would make it easier for farmers to integrate into the EU). This assumption is based on conclusion made by Cechura and Hockmann (2014), where they explain the fact that in number of European countries there is capital-saving technological change instead of expected capital-using technical change as the effect of serious adjustment problems, including problems in the capital market.. Second possible reason for nonsignificant effect of technological change on production elasticity of labor, land and capital is that the financial support of agricultural sector, which was supposed to create sufficient conditions for accomodation of technological progress, has not shown yet. Then, the biased TCH is not pronounced in relation to most production factors. Weather impacts (SUMPit, AVTit) are not in significant relation to technological change. Both types of models, FMM and RPM were discussed in relation to the presence of the heterogeneity effects All estimated random parameters in both RPM models are statistically significant with the exception of the production factor capital in a model that does not involve the influence of weather (model RPM). Estimated parameter for variable KITit (0,0221) shows significant positive impact of the weather on the distribution of TE. That way, heterogeneity in relation to TE is confirmed, too, as well as significant impact of weather on the level of TE. Management (production environment) is significant in all three estimated fixed management models. In models that include weather impacts (AVT, SUMP), the parameter estimates indicates positive, slightly decreasing effect of management (or heterogeneity, respectivelly) on the level of final crop production. In model FMM, on the contrary, first and second order parameters of mangement indicate also significant, but negative and decelerating effect of management (heterogeneity) on final crop production. If weather impact is included into models in form of AVTit, or. SUMPit, the direction of the influence of management on the level of final crop production changes. Based on the significance of first order parameter of management, significant presence of heterogeneity of analyzed sample is confirmed in all three estimated fixed management models. As far as the effect of heterogeneity on single production factors (so called management bias) is concerned, the results indicate that in case of model that does not include weather impacts (model FMM) the heterogeneity has positive impact on production elasticities of land and capital and negative effect on the production elasticities of material inputs. In models that account for weather impacts, heterogeneity has negative effect on production elasticities of land and capital and positive effect on the elasticity of material inputs. Heterogeneity effect on the production elasticity of labor is insignificant in all models FMM. In all three estimated models, the effect of heterogeneity is strongest in case of production factors specific and othe material, and, also, on production factor land. In case of FMM model, heterogeneity leads to increase of production elasticity of land, while in AVT and SUMP heterogeneity leads to decrease of production elasticity of land. At the same time, the production elasticity of land, as discussed earlier, is rather low in all three models. This fact leads to a conclusion that in models that accomodate weather impacts (AVT and SUMP), as the effect of extraction of weather from the sources of unmeasured heterogeneity, the heterogeneity has a negative impact on production elasticity of land. It can be stated that the inclusion of weather effects into the sources of unmeasured heterogeneity overestimated the positive effect of unmeasured heterogeneity on the production factor land in the model FMM. Management does not have a significant effect on the weather in form of SUMPit, while it has significant and negative effect on the weather in form of average temperature, AVTit, with the value of -0.0622**. In other words, heterogeneity is in negative interaction with weather represented by average temperatures, while weather in form of the sum of precipitation (SUMPit) does not exhibit significant relation to unmeasured heteregeneity. In comparison with the model that does not include weather impacts, the effect of heterogeneity on the production elasticities has the opposite direction the models that include weather. Compare to the model where weather is represented by average temperature (model AVT), the effect of management (heterogeneity) on the production elasticity of capital is bigger in model with weather represented by sum of precipitations (model SUMP) while the effect of management (heterogeneity) on the production elasticity of land and material imputs is smaller in model with weather represented by sum of precipitations (model SUMP). Technical efficiency is significant in all estimated models. The variability of inefficiency effects is bigger than the variabilty of random error in both models that include weather and models where weather impacts are not specified. The average of TE in random parametr models reaches rather low value (setting the average TE = 54%), which indicates, that specified RPM models underestimate TE as a possible result of incorrect variable specification, or, incorrect assumptions on the distribution of the error term representing inefficiency. All estimated FMM models results in simmilar value of average TE (86-87%) with the simmilar variability of TE (cca 0,5%). Technological change has significant and positive effect on the level of TE in the model that does not specify the weather impacts (model FMM), with a value of 0,0140***, while in the models that include weather in form of average temperatures, or sum of precipitations, respectivelly, technological change has a negative effect on the level of TE (in model AVT = -0.0135***; in SUMP = -0.0114***). It can be stated, that in the model where the weather impacts were not specified, the effect of TCH on the level of TE may be distorted, because the parameter estimate implies also a systematic influence weather in the analyzed period. The effect of unmeasured heterogeneity on the level of TE is significant in all three estimated fixed management models. In models AVT and SUMP, heterogeneity has a positive effect on the level of TE (in AVT = 0.1413 and in SUMP =0,1389), while in the model that does not include weather variable the effect of heterogeneity on the level of TE is negative (in FMM =-0,1378). In models AVT and SUMP, the weather impacts were extracted from the sources of unmeasured heterogeneity, and so from its influence on the level of TE (together with other production factors weather becomes a source of measured heterogeneity). The extraction of the weather from the sources of unmeasured heterogeneity leads to change in the direction of heterogeneity effects on the level of TE from negative (in model where weather was part of unmeasured heterogeneity) to positive. The direct impact of weather on TE is only significant in case of variable AVTit, indicating that average temperatures reduce the level of TE (-0.0622**). Weather in form of sum of precipitations does not have a significant impact on the level of TE. It is evident that incorporating the effects of weather significantly changes the direction of the influence of management on the production of cereals and the direction of influence on the management of production elasticity of each factor in the final model. Analogically with the case of the influence of heterogeneity on the production elasticity of land, it is stated that the weather (included in sources of unmeasured heterogeneity) played a role in the underestimation of the impact of heterogeneity on the overall cereal production. Also, in case that weather was not extracted form the sources of unmeasured heterogeneity would play significant role in underestimation of the effect of heterogeneity on the level of TE. Based on the results of parameters estimates, and on the estimate of average values of TE and its variability, it is concluded, that the effect of inclusion of weather into defined models does not have significant direct impact on the average value of TE, however, its impact on the level of TE and the level of final crop production is pronounced via effects of unmeasured heterogeneity, from which the weather was extracted by its specification in form of AVTit a SUMPit. The analysis results confirms that it is possible to specify the impacts of weather on the shape and shift of production frontier, and, this to define this impact in a model. Results Aaso indicate that the weather reduces the level of TE and is an important source of inefficiency Czech producers of cereals (crop). The model of stochastic frontier produkction function that capture the weather impact was designed, thereby the goal of the dissertation was met. Results also show that unmeasured heterogeneity is an important feature of czech agriculture and that the identification of its sources is critical for achieving higher productivity and higher level of final output. The assumption about significant presence of heterogeneity in production technology among producers was confirmed, and heterogeneity among producers is a significant feature of cereal sector. By extracting weather from sources of unmeasured heterogeneity, the impact of real unmeasured heterogeneity (all that was not extracted from its sources) and the real impact of weather on the level of TE is revealed. If weather was not specified in a model, the TE would be overestimated. Model in form of translogarithmic multiple-output distance function well approximates the relationship between weather, technical efficiency, and final cereal production. Analysis also revealed, that the Random parameter model, which was applied in case that weather impacts were expressed as an index number, is not the suitable model specification due to underestimating of the average level of TE. The problem of underestimation of TE might be caused by wrong variable definition or incorrect assumptions about the distribution of inefficiency term. Fixed management model, on the other hand, appears as a very good tool for identification of weather impacts (in form of average temperatures and sum of precipitations in the period between planting and harvesting) on the level of TE and on the shape and shift of production frontier of czech cereals producers. The results confirm the assumption that it is important to specify weather impacts in models analyzing the level of TE of the plant production. By specification of weather impactzs in form of proper variables (AVTit, SUMPit), the weather was extracted from the sources of unmeasured heterogeneity. This methodical step will help to refine the estimate of production technology and sources of inefficiencies (or, the real inefficiency, respectivelly). That way, the explanatory power of model increase, which leads to generally more accurate estimate of TE. Dissertation has fulfilled its purpose and has brought important insights into the impact of weather on the TE, about the relationship between weather and intercompany unmeasured heterogeneity, about the effect of weather on the impact of technological change, and so the overall impact of weather specification on the shape and shift of production frontier. A model that is suitable application to define these relationships was designed. Placing the weather into deterministic part of production frontier function instead of statistical noise (or, random error, respectivelly) means a remarkable change in the methodical approach within the stochastic frontier analysis, and, due to the fact that the analysis of weather impacts on the level of TE to this extent has not yet been observed in relevant literature, the dissertation can be considered a substantial contribution to current theory of the estimate of technical efficiency of agriculture. The dissertation arose within the framework of solution of the 7th FP EU project COMPETE no 312029.

Valve controls and their timing
Vajčner, Patrik ; Pexa, Martin (advisor) ; Kotek, Tomáš (referee)
Abstract: Comparison of different types of valve operating mechanisms is the main aim of the Bachelor thesis. This was done from the point of view of the construction manufacturing, with regard to the engine performance, user´s comfort and fuel consumption of a vehicle. There were two types of valve operating mechanisms chosen for the purpose of the Thesis. The first one is a typical classic system whereas the second one is a modern type with a possibility of timing, which allows different timing for valves opening and closing. This process is dependent on the current requirement of engine output. Achievement of the optimal engine output for every specific type of ride is not the main purpose of the correct timing of valves and engine run adjustment in a vehicle. It also allows for a reduction of the excessive engine load and gradual wearing of each part of the system. Of course another very significant reason is to lower the fuel consumption as much as possible with regard to reduction of harmful, environmental unfriendly gases and emissions.

Changes of proline concentration and electrolyte leakage in the selected species of the Panicoideae tribe under water deficit.
Nováková, Hana ; Hnilička, František (advisor) ; Václav, Václav (referee)
The theme of this thesis is the impact of water deficit on the concentration of proline and electrolyte leakage from representatives of the Panicoideae tribe. The impact of drought on agricultural production in the environment of the Czech Republic is currently an important issue. The exceptionall dry year of 2014/2015 and the associated water deficit in the soil had an impact mainly on the harvest of wide-row crops, including maize. Representatives of the Panicoideae tribe - sorghum and foxtail millet are the most drought-resistance crops in the world. Sorghum and foxtail millet belong to II. group of cereals like maize. Sorghum and foxtail millet show lower transpiration coefficient than maize, they also tolerates less demanding growing conditions and are more resistant to diseases and pests. They also compete with maize in a wide range of applications. It is therefore appropriate to determine how these crops respond to water deficit during their ontogenetic development. For the purposes of this experiment genotypes originating from conditions of temperate climate were selected, this genotypes have a shorter growing season. A short growing season is an important factor in the timing of the harvest of the late sown crops in our climate. The aim of the thesis was to determine and evaluate the impact of water deficit on the concentration of proline and electrolyte leakage in selected genotypes of sorghum and foxtail millet and on the basis of the results to select genotypes with high resistance to water deficit. Based on the aims the following hypotheses were set: to determine whether there are differences in the concentration of proline and electrolyte leakage between selected genotypes depending on the effect of water deficit and whether there are differences in these physiological characteristics in response to water deficit between sorghum and foxtail millet. To fulfill the objectives and evaluation the hypothesis was based on the greenhouse experiment with selected genotypes of sorghum and foxtail millet at the Department of Botany and Plant Physiology in the Czech University of Life Sciences Prague. The experiment included 4 genotypes of the species Sorghum bicolor (L.) Moench. (Sorghum): 1216, 30485, Barnard Red and Ruzrok and one variety of the species Setaria italica (L.) Beauv. (Foxtail millet) Ruberit. A total of 4 variants were designated for the experiment (one control and three under the influence of water deficit in different times). The plants in the control variant were full irrigated throughout the experiment. Variant KS was irrigated for the first 14 days, followed by 10 days without watering and the last 4 days were again irrigated. Plants in variant SK were under the influence of water deficit for the first 10 days of the experiment, then 18 days with watering and the plants in the variant SS were without watering for the first 10 days, for the next 4 days were without watering, then the same pattern was repeated: 10 days without and 4 days with watering. Physiological characteristics were observed in plants in the developmental stages BBCH 14-16. The concentration of proline was determined by measuring the absorbance of the samples formed by the reaction with ninhydrin. Subsequently, the measured values were compared with the calibration curve. To measure the sample absorbance at 520 nm a spectrophotometer Hellios gama (Thermo) was used. Electrolyte leakage on discs made from leafs was measured with electrical conductivity meter GRYF 158 (HB Griffin, Ltd.). The obtained results show that the proline concentration increased in response to water stress, since the highest concentration of proline was detected in plants growing in the most stressed variant of SS (670.13 micrograms) compared with other variants of stress. Plants variant KS (643.85 micrograms) showed a higher accumulation than the proline in variant SK (636.69 micrograms). The highest concentration of proline was measured on a genotype in the variety of sorghum Ruzrok (642.94 micrograms). The lowest content of proline was found in the variety of sorghum 1216 (623.78 micrograms), this value was also the only significant difference from the contents of proline to other genotypes. Which at least partly confirms the proposed hypothesis that there are differences in the content of proline between genotypes. The highest electrolyte leakage cell damage was respectively exhibited in plants from the variant SS (39.56%). Plants of the SK variant showed a higher rate of cell damage (32.95%) than plants from the variant KS (29.88%). The lowest cell damage was exhibited in plants grown under control conditions, which again indicates that damage cells increases in response to water deficit. In this experiment the highest degree of cell damage was found in the variety of sorghum 1216 (29.26%) and lowest cell damage was observed in the variety of sorghum Ruzrok (27.03%). A significant different degree of cell damage appeared only in the variety foxtail millet Ruberit (28.6%), which confirms our hypothesis that there are differences in the electrolyte leakage between sorghum and foxtail millet. The hypotheses were not fully confirmed by the experiment. Nevertheless, on the basis of obtained results we conclude that the least drought-resistance genotype is the 1216 variety and the best adapted to the water deficit was the Ruzrok variety. It also confirmed that the higher proline accumulation may protect the plant against the negative effects of water deficit on the cell membranes, which is then reflected in a reduced electrolyte leakage.

Effect of water stress on gas exchange and fluorescence of selected species of tribe Panicoideae
Hrkotová, Kristýna ; Hnilička, František (advisor) ; Václav, Václav (referee)
Summary The thesis studied the effect of water deficit on gas exchange in young plants sorghum and foxtail. These crops could replace maize cultivation in some areas, because their use is similar. Our country is currently facing shortage of rainfall. In 2015, this phenomenon was very noticeable. Therefore, it is appropriate to see how the plants of sorghum and foxtail respond to water deficit during their ontogenetic development. On the water deficit are very sensitive plants in the early stages of growth, and therefore the influence of water deficit was observed on young plants of sorghum and foxtail. Plant genotypes 121, 304, Ruzrok Red and Plant foxtail were divided into four variants: KK (fully irrigated throughout the experiment) KS (14 days full of grout, 10 days water deficit, 4 days full dressing), SK (10 days water deficit, 18 days full of dressing), SS (10 days water deficit, four days filled with grout, water deficit 10 days, four days full of topping). The speed of exchange of gases were measured in the greenhouse device LCpro+ and chlorophyll fluorescence apparatus OS1 - FL. The rate of photosynthesis and transpiration and chlorophyll fluorescence were measured in the morning. The difference between the measured values of the rate of photosynthesis and transpiration value was calculated water use efficiency (WUE). Most sensitive to water deficit responded genotype 121 which had a value of water use efficiency (WUE) 9.95 (10-3) and also had the genotype and the lowest value of chlorophyll fluorescence and 0.765. Resistant to water deficit seems foxtail plants, which had the lowest photosynthesis difference between control and stressed variant in the range of 14.59 mikromol CO2.m-2.s-1 (SS) to 15.38 mikromol CO2.m-2.s-1 (KK). At the same time it also foxtail lowest difference between chlorophyll fluorescence variant KK (0.814) and SS variant (0.781). From the perspective of transpiration as most resistant to water deficit appeared foxtail (0.08 mmol H2O.m-2.s-1 (SS) to 2.13 mmol H2O.m-2.s-1 (KS)). Conversely, the least resistant genotype was 121, which was in the control variant 1.17 mmol H2O.m-2.s-1 and stressed 1.50 mmol H2O.m-2.s-1.

New cryoprotectives in preservation of potentially probiotic bacteria of Bifidobacterium genus using freeze-drying procedure
Vrabcová, Petra ; Killer, Jiří (advisor) ; Jakub, Jakub (referee)
The probiotic bacteria of the genus Bifidobacterium are especially used in the dairy and pharmaceutical industry. They are present in the form of viable cells in pro(syn)biotic fermented milk, dried and freeze-dried products for its positive effects on the health. Lyophilization represents one of the widely used methods for long-term preservation of bifidobacteria. The lyophilization process is challenging, but very needed, and for this reason it is still necessary to explore new cryo(lyo)protective substances, which protect the microbial cultures more effectively before the negative effects of cryopreservation and lyophilization. The aim of the thesis was to test the various mixtures of substances containing specific oligosaccharides, which have prebiotic properties, from the point of view of their potential cryo(lyo)protective effect of lyophilization process. For the purposes of the study, twelve bifidobacterial strains of human and animal origin were selected. As a potential cryo(lyo)protectives were used solutions on the basis of 5x concentrated TPY medium with addition of FOS, 10% fructooligosaccharides (FOS) and the solution on the basis of porcine gastric mucin. Number of viable cells was determined in lyophilisates enriched by the concrete solutions in the time intervals (30 days, 3 months and 6 months) after lyophilization and compared with the control lyophilized samples (fresh cultures without the additive solutions). The potential cryo(lyo)protective effectiveness of 5x concentrated TPY medium solution has been demonstrated only in B. boum, B. breve, B. longum subsp. infantis a Bombiscardovia coagulans, where decreased the number of viable cells about four orders of magnitude. The potential cryo(lyo)protective effectiveness in 10% FOS solution was found only in B. thermacidophilum subsp. porcinum and Bombiscardovia coagulans, where was found half the number of viable cells. The highest potential cryo(lyo)protective effectiveness was observed for a solution on the basis of porcine gastric mucin. The highest number of viable cells was demonstrated in B. animalis subsp. lactis, B. boum, B. longum subsp. infantis, B. longum subsp. longum, B. pseudolongum subsp. globosum and B. thermacidophilum subsp. porcinum lyophilisates using this potential cryo(lyo)protective solution.

Temperament, character, behaviour of the horse and its exploitation
Kohoutová, Zuzana ; Majzlík, Ivan (advisor) ; Hofmanová, Barbora (referee)
Physical disposition determine horse´s capabilities of performance. Mental character of a horse basically controls his behaviour through instincts, which were obtained during millions of years of evolution. Effects of instincts can be enhanced or softened by highly developed senses. Thanks to this special combination, mankind tends to give horse an unique approach. Every individual is different and the temper may vary, it also depends on a breed. Temper determines his behaviour towards other horses and people as well, it sets prerequisitions for further use of a horse, f.e. active horses of breed A1/1 (Thoroughbred) are much more likely to be used as a racehorse than the phlegmatic ones. Horses can be divided by geografic origin into 4 groups: A group of Mongolian horses (prairie) A group of horses Eastern (Oriental), the ancestor of the tarpan a) subgroup horses Iran b) subgroup horses tarpans c) Arabian horses subgroup of type d) subset horses English Group of western horses, their ancestor's western type horse (now extinct) A group of horses Nordic (Nordic) Other division can be made by temper: Coldblooded horses, Equus robustus is the ancestor, neco, calm, obedient, mostly used for work. Hotblooded horses, Equus ferus is the ancestor, neco, vital, used as a sport horses. Can be purebred or crossbred. (in this context, crossbred only with certain allowed breeds)

Control of web analytics implementation
Kocourek, Tomáš ; Šimek, Pavel (advisor) ; Ladislav, Ladislav (referee)
The diploma thesis is focused on verifying the accuracy of web Analytics Adobe Analytics. Manual control is a time-consuming activity, therefore web analysts are looking for ways leading to its acceleration and automation. Selected problem was solved by writing VBA macro in MS Excel. Macro checks the reports that are uploaded into Excel from analytic database using Adobe Report Builder. The theoretical part is focused on explaining the role of web analytics in online marketing and related concepts. The practical part presents the requirements for macro in Excel and then interprets all functions and procedures created by the program. Thesis also includes the configuration of the ObservePoint system competitive environment, which will be the last part compared with created macro. Created solution in Excel allows to verify the accuracy of web analytics with clearly sorted results quickly, thereby replaces the previous manual control.

Molecular mechanism of hydrogen sulfide action during meiotic maturation of porcine oocytes
Veselá, Andrea ; Hošková, Kristýna (advisor) ; Tomáš, Tomáš (referee)
At present reproductive biotechnology methods are on the rise, but their development and application in the broader management of reproduction is dependent on obtaining a sufficient number of quality oocytes cultured in vitro. The prerequisite for this requirement is the creation of the optimal conditions in the course of culturing oocytes. Understanding and knowledge of the processes that occur in oocyte during maturation is an important and necessary condition for optimizing the process of culturing oocytes in vitro and gaining a sufficient number of good quality oocytes in metaphase II of meiotic division. A large number of mechanisms that affect and control oocyte maturation are known, however it cannot be claimed that this process has been fully explained and studied. One factor which has a potential role in the regulation of meiotic maturation of oocytes is gasotransmitter hydrogen sulfide (H2S), a critical signaling molecule of endogenous origin. The study of H2S led to the hypothesis that H2S actively influences the course of meiotic maturation of pig oocytes by regulating key signaling cascades. The aim of this work was to determine the involvement of H2S in the regulation of the MEK1-MAPK signaling cascade, responsible for the initiation and progress of the meiotic maturation of oocytes and the MEK1-PARP-1 cascade as signaling that supports cell viability. For this purpose, pig oocytes cultured in modified media were used, supplemented with a specific combination of enzyme inhibitors (3Ki) or in a culture medium with donor H2S. The ocytes were then subjected to immunocytochemistry staining, fluorescence microscopy and image analysis. The results show that H2S is involved in the regulation of meiotic maturation. It confirmed the hypothesis of the endogenous production of H2S in the course of the meiotic maturation of pig oocytes and the influence of the MAPK signaling cascade. Based on the results, it is however likely that the MEK1-PARP-1 signaling cascade is not affected by H2S, unlike MAPK signaling, comprising the mentioned MEK1 as superior kinase. MAPK kinase activity is significantly lower in oocytes after treatment 3Ki. Further experiments are for a detailed understanding of these regulatory pathways and for the proper verification of the mechanism of the effects of H2S necessary, in particular for a full understanding of the target control factors by the post-translational modification of S-sulfhydration.