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Arching
Willertová, Aneta ; Dolanová,, Lenka (referee) ; Klímová, Barbora (advisor)
The topic of this bachelor's thesis is the formation of so-called "personal mythologies" - subjective constructs of reality which are created spontaneously and naturally as a result of the need of inner and outer world interpretation. These constructs are built on the groundwork of experiences, impressions and memories, associating organically and creating unique bonds. The work put a goal to make this topic as an environment which will show the spectator those bonds, background of their formation and final mythologies as a selfportraits unit.
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High Voltage Pulse Generator for Electroporation of Cells
Puczok, Václav ; Martiš, Jan (referee) ; Červinka, Dalibor (advisor)
The main goal of this thesis is to design control board for the experimental electroporation device and to develop control firmware. The first chapter of this work focuses on the electroporation phenomenon itself. Behaviour of the cell model in external electrical field is described there as well as simulation and overview of how electroporation affects living tissue. It also explains the main requirements for parameters of the electroporation pulses as well as need for ECG synchronization. Furthermore, some remarks are given about novel high frequency electroporation method, which involves use of nanosecond bipolar high voltage pulse bursts. The second chapter briefly introduces commercial electroporation device called Nanoknife, including control part, power part, and it's limits. The third chapter consists of introduction of the novel experimental electroporation device developed at BUT. Power part of this device is discussed as well. Next chapter focuses on design of the control board for this device and also on description of the particular schematic parts. There is a control algorithm explanation in the fifth chapter of this thesis followed by the brief manual to machine operation.
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Microarray Data Interpretation
Ludwig, Petr ; Šilhavá, Jana (referee) ; Smrž, Pavel (advisor)
This Bachelor thesis explains the basics of biochip or microarray data interpretation, starting with short introduction to genetics, especially genetic information significance evaluating. The focus was set mainly on the set of scripts transforming and analyzing the sample data. The data used in this thesis are a result of biochip analysis of the Colon Tumor tissues. The secondary result represents disclosing the main marker for this particular type of cancer, the primary result is evaluation of marker significance in the context of signaling pathways. The resulting pathways are sorted by relevance.
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The effect of therapeutical ultrasound and metalic nanoparticles on carcinoma cells
Kalužová, Adéla ; Rozman, Jiří (referee) ; Bernard, Vladan (advisor)
This thesis focuses on the research of an effect of ultrasound and nanoparticles on cancer cells. In the literature search there the applications of ultrasound field in the interaction with aplication of metallic nanoparticles to viability of cancer cells are described, on the basis of in vitro experiments‘ performance. The purpose of metallic nanoparticles‘ application should be a possible reinforcing of its antitumor effect thanks to the presence of ultrasound field. In next part of the literature search there are included the technical performance of devices, their indications and contraindications, parameters of ultrasound fields and application of metallic nanoparticles. The metallic nanoparticles have been becoming a part of the human’s life more and more, that is why it’s necessary to deal with these miniature particles at a higher level. The aim of this thesis is to suggest the ways of resolution based on the principle of sounding of cells with the ultrasound field of suitable parameters and in combination with the application of metallic nanoparticles with a successive performance of the cell viability tests. The viability was determinated by the MTT assay test. Another part of this work is the measurement of parameters of this zone by means of a submersible needle hydrophone, for a better understanding of the ultrasonic zone. In the final part of this work there is necessary the statistical evaluation of the measured data of the experiments carried out and the discussion of the results obtained.
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Signal Processing for Diffusion Weighted Imaging
Petrek, Tomáš ; Bartušek, Karel (referee) ; Marcoň, Petr (advisor)
Diploma thesis explores the possibility of using diffusion-weighted images in medicine. The paper is a brief physical principle of operation of the magnetic resonance as a tool for non-destructive imaging of the internal structure of substances, the principle of the display contrast as T1, T2 and diffusion weighted images, the course of the sequence for obtaining images with different contrast. Medicine is faced with the problem of classification of pathological tissue in the brain. Contrast diffusion-weighted images does not visually determine the shape of pathological tissue in the form of a tumor or edema. With the T1 and T2 weighted images were calculated mask corresponding tumor and edema, that have been applied to the diffusion-weighted images. Images of the tumor and edema have been subjected diffusivity measurements and statistical evaluation for the purpose of classifying the type of tumor. Investigations were seven findings glioma and metastatic five awards. The research was focused on classifying pathological tissue.
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Radiotherapy of breast cancer
TRAKALOVÁ, Barbora
Diagnosis code C50 represents malignant neoplasm of breast. It is the most frequent tumour disease in women. In 2009, 5975 cases were newly diagnosed in women in the Czech Republic and 1607 women died of this disease. There exist many risk factors which can cause tumour such as hereditary disposition, age, menses, menopause and other factors. At the same time, we can influence many factors and so prevent the disease. Prevention is also important, whether in the form of self - investigation of breast or mammography screening, which is recoverable by insurance from the age of 45. Treatment of breast carcinoma includes a lot of medical modalities such as surgical, hormonal and biological treatment, chemotherapy and radiotherapy. This thesis deals in more details with radiotherapy which is irreplaceable in treatment of this disease. Radiotherapy is a treatment of malignant and benign tumours by means of ionising radiation. In order to treat patients with radiotherapy, a team of doctors and radiologists shall create an individual radiation plan for them including the exact target volume, critical organs, radiation technology, fractionation and particular radiation exposure, radiation position and many other data necessary to accurate irradiation. A technology of two tangential fields is the most applied technology at breast cancer treatment. This technology is also applied in Nemocnice České Budějovice a.s. where I have created a file of 60 patients with breast carcinoma treated on oncology department in the year 2011. There have been used 536 verification images on the whole, it means 1608 evaluation. In course of treatment a patient should have an identical radiation position, which is reached with irradiation and fixation aids. However, from various reasons small or bigger deviations from the optimal position happen and therefore a possibility of irradiation of another volume than the required target one can occur. It is necessary to be aware of the fact that during a treatment of oncology patients there can often appear change of weight, change of body shape of a patient and at advanced stages even also change of physical state and movability of a patient. Therefore large deviations can happen in patients with breast carcinoma namely for example in case of breast deformation or abnormal loss of weight due to the treatment. In Nemocnice České Budějovice a.s., the maximum allowed deviation for vertical, lateral and longitudinal axis is stated 0,7 cm and for rotation 3°. I have noticed the acquired deviation values from verification images into a chart and consequently into a figure where are shown the average deviations and frequencies of these deviation in particular axes for each radiation field. The biggest deviation has happened in vertical axis and the average deviation in that axis was 0,29 cm. The maximum allowed deviation was exceeded only at 3 %, so at 45 deviation from the total evaluation. This thesis should prove the fact that verification images enable to prevent possible errors during radiotherapy. This hypothesis has been confirmed because only 3 % evaluation exceeded the maximum allowed deviations. Verification images enable us to disclose wrong irradiation position, therefore only the target volume is irradiated and follows no irradiation of the surrounding healthy tissue and critical organs.
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The role of the nurse taking care of patients with breast cancer.
KRBCOVÁ, Julie
The actual issue regarding the female population consists in a high incidence of pathological breast findings that can be benign but even most frequently malign. For my bachelor thesis, I chose the theme "The Role of a Nurse in Providing Nursing Care for Women with Breast Tumors", because these issues remain still topical. Basic theoretical grounds of the thesis describe female breast anatomy and pathology. They inform us about problems regarding the causes of the tumor disease and the most common symptoms of the disease. The use of up-to-date diagnostic methods (primarily mammography) and their wide accessibility within a screening program take a significant part in a high percentage of early detected stages of the disease. Breast tumor surgery belongs to the operations demanding from both physical and psychic aspects. The role of a general nurse in providing preoperative and primarily postoperative care is important. A nurse provides a complex nursing care, observes nursing care principles to satisfy individual patient? needs. A nurse does not forget to give information, minds the education, sympathetic access and psychic support of a patient. This bachelor thesis was devoted to the issues of a nurse role in providing postoperative nursing care to patients with breast tumor. The first objective was to describe specific aspects of nursing care in patients after surgery for breast tumor. The second objective was to find out the role of a nurse within a postoperative period in patients with breast tumor. Two research questions were set to fulfill those objectives. The first research question: "Which specific nursing activities does a nurse perform within a postoperative period in patients after breast surgery?" The second research question: "What role does a nurse hold within a postoperative period in patients with breast tumor?" A qualitative research inquiry was used to process the research part of this bachelor thesis. A semi-structured interview was used as a data collection method. The research inquiry was performed in the Department of Surgery of the České Budějovice Hospital, Inc. The first research question surveyed specific nursing activities of nurses performed in patients after surgery for breast tumor. The answers showed that nurses were familiar with specific nursing activities which they performed within postoperative period in patients with breast tumor. Specific aspects described by both the nurses and patients are narrowly associated with the intervention they underwent and which is specific itself. The second research question found out the roles of a nurse within a postoperative period in patients with breast tumor. From the results of answers given by both nurses and patients is clear that the following roles of a nurse were fulfilled: a communicator, an educator and a bearer of changes. Some educational and illustrative tools (silicone breast phantom for the self-examination instruction) intended for nursing personnel are one of the possibilities how to achieve an effective application of nursing interventions in breast tumor prevention and how to help nurses to improve the relation of communication and education on these issues. The results of my bachelor thesis could be used as an information material that could serve to an improvement of the quality and effectiveness in nursing care for the experienced nurses as well as for beginning ones who meet such patients at their job. The results of my research inquiry could be used as basic material to elaborate some lecture for a professional seminary or conference intended for nurses.
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