National Repository of Grey Literature 75 records found  beginprevious51 - 60nextend  jump to record: Search took 0.01 seconds. 
Physiological relevance of futile cycling based on lipolysis and fatty acid re-esterification in white adipose tissue
Funda, Jiří ; Flachs, Pavel (advisor) ; Kolář, David (referee)
AJ The thesis deals with the task of futile metabolic cycling, mainly on the cycle including lipolysis and fatty acid re-esterification, which takes place in white adipose tissue. This cycle plays some essential roles in organism, including regulation of important metabolic pathways in lipid metabolism and also exhibit certain influence on the whole body energy metabolism. First part of the thesis is focused on general properties of futile metabolic cycles and shows some examples of their functions in organism. Next part presents detail view on single steps making the whole lipolysis/re-esterification cycle. Considerable part deals with the ways of regulation of futile cycle activity. This approach may increase an impact of futile cycling on processes under its influence. Physiological relevance of futile metabolic cycle based on lipolysis and fatty acid re-esterification in white adipose tissue was described in numerous studies. This thesis shows their results for a purpose to provide a summary of functions of this system in physiology of mammals. Key words futile metabolic cycle, lipolysis, re-esterification, fatty acids, adipose tissue
Factors affecting glucose metabolism and inflammatory response in critically ill patients
Kotulák, Tomáš ; Haluzík, Martin (advisor) ; Maruna, Pavel (referee) ; Šenolt, Ladislav (referee)
Hyperglycemia in critically ill patients was considered for many years an adaptive response to stress conditions being present in both patients with and without previous history of diabetes. Hyperglycemia is caused mainly by peripheral insulin resistance induced by the factors acting counteracting insulin signalling at the postreceptor level. Furthermore, hyperglycemia itself can then increase serum levels of pro-inflammatory cytokines such as tumor necrosis factor alpha (TNF-α), interleukin-6 (Il-6) and interleukin-8 (Il- 8) and others. On the contrary, peripheral insulin resistance induced by pro- inflammatory cytokines may further potentiate hyperglycemia. White adipose tissue represents in addition to its energy storage function also a very active endocrine active organ. In addition to regulation of a number of metabolic processes it also significantly modulates the inflammatory response. In critically ill patients, adipose tissue changes its morphology, i.e. the adipocytes are shrinking and adipose tissue is abundantly infiltrated by macrophages. Paradoxically, overweight and obese critically ill patients have lower mortality than underweight, lean and morbidly obese subjects. In our studies, we selected population of the patients undergoing elective major cardiac surgery with extracorporeal...
The role of immune system in development of obesity and derived metabolic complications
Jindrová, Simona ; Bardová, Kristina (advisor) ; Cinkajzlová, Anna (referee)
Obesity is a chronic disease caused by an imbalance between energy intake and expenditure. Important source of energy is the body fat, stored in adipose tissue cells in the form of triacylglycerols. The adipose tissue does not only store triacylglycerols but also functions as an endocrine organ with the ability to secrete cytokines and adipokines - the molecules, participating in series of the metabolic proceses. The consequence of high-fat diet feeding is the growth of adipose cells and altered production od adipokines and cytokines. Majority of them have proinflammatory functions. Consequently inflammation of adipose tissue is increased as well as the activation of components of immune system, especially macrophages. The interplay between these and other aspects, including hight free fatty acid plasma levels, also associated with metabolic disorders caused by obesity, is connected with the development of insulin resistance, that means state, when the tissues are nonsensitive to insulin. Key words: obesity, adipokines, cytokines, immune system, adipose tissue, insulin resistance
The role of adipose tissue in the pathophysiology of obesity and anorexia nervosa
Kratochvílová, Helena ; Haluzík, Martin (advisor) ; Pavlišová, Jana (referee)
The view of adipose tissue and its significance in human pathophysiology has changed substantially in the recent couple of years. It has been shown that adipose tissue is not only a passive storage site for excess energy, but that it actively participates in the maintenance of whole-body homeostasis as an endocrine organ producing various hormones and other biologically active molecules, which by acting in an endocrine, paracrine as well as autocrine fashion affect different organs and systems. An intact regulation of adipose tissue processes plays an important role in sustaining the integrity of the organism, whereas its defects lead to the development of a number of diseases. This thesis focuses primarily on the significance of adipose tissue in the pathogenesis of obesity and anorexia nervosa. In the last years, the incidence of obesity rapidly increases ranking, along with its complications, among the world's leading health problems. Obesity is associated with endocrine dysfunction of adipose tissue characterized by disrupted production of adipose tissue hormones and cytokines resulting in the development of numerous disorders including type 2 diabetes mellitus and cardiovascular diseases. A completely opposite disorder, where, however, adipose tissue, or more precisely the absence of it, plays...
Stress of endoplasmic reticulum and its role for the development of adipose tissue inflammation
Zemánková, Kateřina ; Rossmeislová, Lenka (advisor) ; Janovská, Petra (referee)
Endoplasmic reticulum (ER) is a cellular organelle responsible for folding of proteins that are then transported to the various places in the cell or secreted. It is also crucial for the synthesis of triglycerides. Metabolic imbalance leads to ER stress and consequently triggers signaling pathway, which is called unfolded-protein response (UPR). The aim of this pathway is to alleviate ER stress, restore natural homeostasis and prevent death of cell. At the same time however, it activates stress kinases and other factors that may perturb insulin signaling and increase expression of proinflammatory cytokines. These signs are characteristic for human obesity, which is associated with reduced tissue's sensitivity to insulin and is considered as a disease with low level of inflammation. Recent studies have suggested that the source of proinflammatory cytokines in obesity are stressed adipocytes and macrophage infiltrated into adipose tissue. Indeed, it has been demonstrated that stress of endoplasmic reticulum is significantly increased in adipose tissue of obese individuals. Weight loss associated with reduction of adipose tissue mass decreases stress while lowers both, the production of proinflammatory cytokines and insulin resistence. This work aims to collect and discuss these new findings, which...
New regulatory metabolic factors in patients with obesity and type 2 diabetes mellitus
Matějková, Mirka ; Haluzík, Martin (advisor) ; Flachs, Pavel (referee)
Fibroblast growth factors are proteins with diverse biological function in development, tissue repair, and metabolism. The human FGF gene family consists of 22 members. FGF 19 subfamily includes FGF 19, FGF 21, and FGF 23. They act as systemic factors in an endocrine manner. FGF 19 subfamily requires klotho protein as a cofactor for its action. FGF 19 produced by intestine acts mainly in the liver through FGFR4, where it inhibits bile acid and fatty acid synthesis. FGF 21 is produced by the liver and contributes to the regulation of carbohydrate and lipid metabolism through modulation of glucose uptake in adipocytes. Serum FGF 21 levels are increased in patients with obesity and type 2 diabetes mellitus. Serum FGF 19 levels are on the contrary decreased in patients with obesity and type 2 diabetes mellitus and more probably depend on the nutritional status of the organism than on the glucose metabolism and insulin sensitivity. Key words: type 2 diabetes mellitus, FGF 19, FGF 21, obesity, adipose tissue
Acipimox during Short-Term Exercise Exerts A Negative Feedback of Growth Hormone on Ghrelin Secretion in Patients with Bulimia Nervosa and in Healthy Women: The Role of Lipolysis
Smitka, Kvido ; Nedvídková, Jara (advisor) ; Kršek, Michal (referee) ; Čáp, Jan (referee)
Title: Acipimox during Short-Term Exercise Exerts A Negative Feedback of Growth Hormone on Ghrelin Secretion in Patients with Bulimia Nervosa and in Healthy Women: The Role of Lipolysis Objective: Eating disorders, such as bulimia nervosa (BN) and anorexia nervosa (AN), are characterized by abnormal eating behavior. The main features of BN are binge-eating and inappropriate compensatory methods to prevent weight gain. The appetite-modulating peptide ghrelin is secreted by the stomach and shows a strong release of growth hormone (GH). A potential GH-ghrelin feedback loop between stomach and the pituitary has been recently reported. Acipimox (Aci), an analogue of nicotinic acid, inhibits lipolysis in adipose tissue (AT) and reduces plasma glycerol and free fatty acids (FFA) levels. Exercise and Aci are stimulators of GH secretion. We suppose that a negative feedback from increased GH levels during exercise may play a role in reducing plasma ghrelin levels. We surmised that altered baseline activity and exercise-induced activation of the sympathetic nervous system (SNS) results in excessive stimulation of lipolysis associated with negative energy balance and may lead to abnormal AT metabolism in patients with BN. Disruption of the gut-brain-AT axis might be involved in the pathogenesis of BN. The...
The effect of live weight of pigs on the histology of adipose tissue
Žalmánková, Nikola ; Okrouhlá, Monika (advisor) ; Michaela, Michaela (referee)
The main goal of this paper was to evaluate the influence of live weight on both the quantity and the quality of back fat as well as on histology of adipose tissue in pigs. Fourty pigs of PIC genotype and equaly distributed sex were included in this study. The population was then divided into four sub-groups according to their live weight. (Groups: first - under 104.9 kg, second - between 105 kg and 109.9 kg, third - between 110 kg and 114.9 kg, fourth - above 115 kg) The following indicators of fattening performance were monitored during the study: average daily feed consumption, average weekly feed consumption, average daily weight gain and feed conversion rate. Statistically significant influence was observed only for average daily weight gain which was found to be positively dependent on live weight increases. The smallest average daily weight gain (990.14 g) was observed in group one which at the same time displayed highest feed conversion rate (2.61 kg.kg-1). Subjects in group four, on the other hand, showed the largest average daily weight gain (1159.11 kg) and the lowest feed conversion rate (2.49 kg.kg-1). Furthermore, development of back fat was periodically monitored via ultrasound examinations at three chosen points of the pigs' anatomy, beginning at four weeks of age and continuing until ten weeks of age. The measurements confirmed that the increases in depth of back fat correlate closely with gains in live weight. No correlation was proven between other physical characteristics of back fat and changes in live weight. However, measurements showed statistically non-significant increase of coloration of back fat which correlated with weight gains for subjects under 114.9 kg of live weight. As far as tenderness of back fat is concerned, lowest values were found in both upper (74.31 N) and lower (59.61 N) fat in subjects in group two. No significant influence of live weight on the histology of adipose tissue was found. Nevertheless, measurements showed apparent structural difference between the cut in the higher and lower part of back fat. In upper part of the cut was the highest density of adipose cells (60.27 cells per 1 mm2 at the average cell size of 3430.23) found in group number two. However, in the lower part of the cut was the highest density of adipose cells (63.8 cells per 1 mm2 at the average cell size of 3877.72) found in group number one. None of the other monitored variables proved to be statistically significant.
The comparison of fatty acids profile in adipose tissue of pigs with regard to the differently enriched feed with unsaturated fatty acids
Homolková, Dana ; Okrouhlá, Monika (advisor) ; Michaela, Michaela (referee)
This paper deals with a comparison of the profile of fatty acids in adipose tissue pigs with regard to the differently enriched feed of unsaturated fatty acids. In the theoretical part, the issue is handled using professional literature, supplemented by chapters dealing with the digestive system and digestion. In the practical part, the experiment was conducted. The experiment was performed at the pig breeding test station at Ploskov near Lány. The experiment included 72 pieces of slaughter pigs of balanced sex (36 barrows and 36 gilts) final hybrid combinations DanBred. This paper presents a methodology, under which the test was performed. The pigs were included in the test in the average weight of 29.2 kg and the average age of 70 days. The pigs were fed with a complete feed mixture. The pigs were according on diet divided into six experimental groups with supplemented 4% oil (rapeseed and soybean) and one control group without added oil. In experimental groups were incorporated oil into the feed mixture, for a period of 6, 4 and 2 weeks prior to slaughter. Age and average slaughter weight of pigs at the end of the fattening period was 152 days and 115.8 kg. From the measurement results, it can be stated that the resulting interaction between the time of application and the type oil used by the addition of has been found in the content of palmitic acid, linoleic acid and alfa linolenic acid, n3 PUFA and n6 PUFA and the ratio of n3 and n6 PUFA and also in oxidation stability, carried out immediately after thawing. Finally, it is possible to say that the profile of fatty acids contained in the feed is reflected in the profile of fatty acids in adipose tissue. When the rapeseed oil was fed the content of MUFA increaset and when the soybean oil was fed the content of PUFA increaset in the adipose tissue. Time of feeding fatty acids had no content great influence. Thus, from economic point of view the best option seems to be the addition of soybean oil in the diet two weeks before slaughter.

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