|
Legal Regulation of Expropriation from the Investor's and Expert's Perspective
Tesařová, Zdeňka ; Superatová, Alena (referee) ; Hanák, Jakub (advisor)
The thesis deals with the institution of expropriation and explanation of basic terms. Subsequently is explained the enactment of the institution of expropriation focusing the role of the investor and the expert. Expert sets according to the expertise report the amount of compensation for expropriation and expenses connected with it. It also specifies the participation and tasks of the investor, who enters the expropriation. The aim of this thesis is to take into account questionable aspects of participation of the investor and the expert in the expropriation proceedings and to adumbrate their possible solutions.
|
|
Cloning, expression and purification of human AKR1B1
Lundová, Tereza ; Wsól, Vladimír (advisor) ; Zemanová, Lucie (referee)
Charles University in Prague Faculty of Pharmacy in Hradec Králové Department of Biochemical Sciences Candidate: Tereza Lundová Supervisor: Prof. Ing. Vladimír Wsól, Ph.D. Title of diploma thesis: Cloning, expression and purification of human AKR1B1 Aldose reductase (EC 1.1.1.21) AKR1B1 is one of the 13 human enzymes of the AKR superfamily. All human AKRs are cytosolic and NADP(H) dependent. AKR1B1 plays an important role in metabolism of endogenous and exogenous substances. The main endogenous substrate is glucose. Its reduction to sorbitol is consistently linked to secondary diabetic complications. From xenobiotics metabolized by AKR1B1, daunorubicin, an anticancer drug from the group of anthracyclines, is reduced to daunorubicinol. This metabolite is less active than parent drug and is the cause of anthracycline related cardiotoxicity. At present, many projects are focused on AKR1B1 as a target enzyme and specific inhibitors of AKR1B1 are looking for. The recombinant protein of AKR1B1 was prepared in E. coli together with the pET expression system. First, cDNA for AKR1B1 in pOTB7 was isolated from E. coli. The coding sequence of AKR1B1 was amplified by a PCR. PCR was performed with Phusion Hot Start II polymerase and pair of forward and reverse primers, which contained NdeI and XhoI restriction...
|
|
Protection of domain names
Dušek, Ondřej ; Horáček, Vít (advisor) ; Horáček, Tomáš (referee)
In the diploma work, there are examined domain names, which have become, due to a mass development of the Internet, an economically very valuable estate. Domain names lack, although being a generally known notion, any legal anchoring in the Czech system of law. The absence of legal regulation of domain names is not an anomaly of the Czech legal environment, but it can also be seen in the states with advanced legal culture. Domain names fulfill, in addition to being a very valuable commercial article, identification and/or promotional functions. Just for those reasons, the domain names need to be provided a corresponding legal protection. The introductory part of the diploma work, containing an introduction into the issues of domain names, addresses technical aspects of domain names and the method of their hierarchical structuring. Further, the diploma work contains a list of institutions which play, in the area of domain names, an important role for various reasons. The historical development of the institutions, their organization, and activity contents are briefly described. Then the legal nature of domain names is examined and a question whether a domain name can be considered to be a thing/item, right and/or any other asset value is answered. Furthermore, the diploma work contains a description...
|
|
Strategic analysis
Pavlíček, Karel ; Krause, Josef (advisor) ; Brabec, Martin (referee)
The goal of this master thesis is execution of a strategy analysis. The subject of this analysis is electro-mobility i.e. products and services related to electric vehicles in the Czech Republic. The theoretical part of the paper is focused on approaches used for analyses of both external factors and forces affecting particular industry. The former is mainly focused on technology, regulations and incentives. The latter analysis is based on five forces supplemented by the role of complements. Actual status of the technology an analysed product is assessed by SWOT analyses. In the conclusion, final recommendations based on overall findings are stated.
|
|
Study of effect of Bacillus subtilis yxkO gene on motility during stress response to osmotic upshift.
Streitová, Eliška ; Lichá, Irena (advisor) ; Krásný, Libor (referee)
Bacillus subtilis is gram-positive soil bacteria. In its natural environment it is constantly exposed to changes of chemical and physical conditons, including changes of osmolality. It responds to high osmolality by transporting of potassium ions and afterthat transporting and/or synthetising of compatible solutes. In last years the mutant strain Bacillus subtilis L-42 was isolated with non-specific insertional mutagenesis (mini Tn10) in our laboratory. This strain displays limited growth and inability to cope with hyperosmotic shock in a defined medium with potassium concentration of < 1 mmol/l. Insertion of transposon was located in yxkO gene which encodes a protein of unknown biological function. Some other data also indicate a possible role of disruption of yxkO gene in regulation of expression of hag gene, which encodes flagelin - a pivotal protein of bacterial flagellum. The goal of this thesis was to clarify if the disruption of yxkO gene influences motility and whether is affected the transcription of hag gene. With integrative vector pMUTIN4 a mutant strain with specific mutation of yxkO gene was prepared. Vector was pasted into chromosome of Bacillus subtilis strain 1A839 - genotype of this strain allows to extrude the known transcriptional regulation of hag gene. Cell's motility was...
|
|
The mechanism of cell death induction in human malignant melanoma in vitro
Rudolf, Kamil ; Červinka, Miroslav (advisor) ; Mlejnek, Petr (referee) ; Janisch, Roman (referee)
Melanoma is a complex malignant disease characterized by increasing incidence and mortality. Melanoma biology is complicated and comprises many aberrant genes and signaling pathways. Treatment of melanoma relies on surgery, the role of radiation therapy and chemotherapy are limited. In advanced disseminating and metastasizing melanoma chemotherapy largely fails and that is why new approaches are being sought including identification of deregulated molecules or processes which could be used as targets. Some of these targets center on frequently aberrant cell death regulating mechanisms. Some traditional cytostatic drugs such as inhibitors of topoisomerases although useful in other types tumors so far proved useless in treatment of melanoma. Nevertheless, expression of topoisomerases in melanoma is often high, thereby enabling at least theoretically their use as targets for chemotherapeutical intervention. Aim of this study was to assess the cytoxicity and proapoptotic effect of campthotecin (topoisomerase I inhibitor), etoposide (topoisomerase IIα inhibitor) and their combination in selected human melanoma cell lines. We found that campthotecin may induce apoptosis by a combination of mechanisms including p53 as well as p73 and kaspase-2. Etoposide-induced cell death in Bowes melanoma cells included...
|
|
Medical Aspects of Abroad
Adli Hanna, Dagmar ; Malinová, Jana (advisor)
Transmitted diseases are generally well controlled. A high level of immunization has been reached and preserved as well. Despite the falling prevalence of schistosomoiasis in the last few years, it remains together with hepatitis and tuberculosis a significant problem of public health. Heath of mothers and children are still a problem. Maternity mortality and neonatal mortality are still high. Not transmitted diseases are increasing, concerning cardiovascular, metabolic, chest-trouble and malignant tumour. The most often malignity is tumour of breast, liver, urinary bladder and lymphoma. Colon tumours are increasing. An important role in mortality plays accidents. About 1, 2% of the whole population is blind, mainly as a consequence of a cataract or a granular lid that is still quite common in some areas. The importance of diseases connected with lifestyle is increasing. Smoking, too much alcohol, fat and sweet nurture, lack of exercise, not using safety belts in cars and not observing traffic regulations participate in total mortality and sickness rate. The following list includes the most important facts: 1. population growth, 2. endemic and contagious diseases, 3. Maternity mortality, neonatal mortality and children mortality, 4. chronic disease, chronic renal failure and tumours, 5. Accidents and...
|
|
Functional analysis of syntaxin 16 phosphorylation using yeast as a model
Volfová, Barbora ; Entlicher, Gustav (advisor) ; Dráber, Petr (referee)
4 Abstract Mechanism of fusion of intracellular membranes in eukaryotic cells involves several protein families including soluble N-ethylmaleimide-sensitive-factor attachment protein receptor (SNARE) proteins and Sec1/Munc-18 related proteins (SM proteins). It is known that the transport is evolutionary conserved from yeast to man. Therefore for facilitating of the research, we can use simple eukaryotes Saccharomyces cerevisiae. Mammalian SNARE protein syntaxin 16 has a yeast homologue Tlg2p which is used in this study as a model for studying affects of phosphorylation to the syntaxin 16 function. Also their binding partners, SM proteins mVps45p (mammalian) and yeast Vps45p are homologous. Phosphorylation of SNARE proteins is known as a possible way of regulation of membrane fusion. Abolishment of one of the putative phosphorylation sites in Tlg2p protein, serine 90 leads to dominant effects on the exocytic and endocytic pathways. The work presented in this study shows some phenotypes of mutants based on this phosphorylation site of protein Tlg2p. Those mutants are S90A (cannot be phosphorylated) and S90D (phosphomimetic - acid carboxyl group mimics phosphate group). It was revealed that the phosphorylation of Tlg2p protein at serine 90 or the mutation Tlg2p-S90D may play some role in protecting Tlg2p...
|
|
Stoichiometry of transport mediated by bacterial membrane transport protein MntH of E. coli
Doležalová, Iva ; Chaloupka, Roman (advisor) ; Marešová, Lydie (referee)
The Nramp family (Natural Resistance-Associated Macrophage Protein) includes secondary active transport proteins transporting divalent metal ions together with protons into a cell. Divalent metal ions play a major role in the cell metabolism and participate in many intracellular processes. We have studied bacterial Nramp ortholog - MntH (Proton-dependent Manganese Transporter), its transport stoichiometry and other functionally important characteristics. We have found that the external pH affected the transport stoichiometry. The functional differences between the wild type and the protein with mutation N401G appears only in lower external pH. Moreover, uncoupled proton flux mediated by MntH under specific experimental conditions is inhibited by calcium, whereas coupled proton flux increases after the addition of calcium ions. This can be connected to the fact that calcium is also transported by MntH. Surprisingly, the transport stoichiometry of neither the wild type nor the protein with mutation N401G is affected by the addition of calcium or the change of the external medium.
|
|
ANALÝZA OSOBNÍ ŽELEZNIČNÍ DOPRAVY V ČESKÉ REPUBLICE OD ROKU 2003 DO SOUČASNOSTI
Jaroš, Vít ; Štípek, Vladimír (advisor) ; Chmelová, Pavla (referee)
The goal of this thesis is to analyze the market of public railway transport in Czech Republic from the point of view of market model and configuration during the period from 2003 to this day. In 2003, Czech Railways was estabilished as a joint-stock company; at the same time Railway Infrastructure Administration was estabilished as a state-owned company. The theoretical part of this thesis defines key economical concepts related to the market structure of public railway transport and to the economical policies of Czech Republic. The main focus are various forms of regulation and imperfect comptetition. The next section serves as a historical overview of railway construction and railway transport in the 19th and the 20th century. This section also introduces the reader to institutions that play vital role in railway transport and that have impact on its development; the reader is also introduced to strategical documents that propose changes and improvements to railway transport and that take into account future development of railway transport in the Czech Republic as well as the European Union. The practical part of this thesis presents an analysis of the development of railway transport in recent years following its liberalization in the Czech Republic. In the last decade we have seen a rise in the number of privately owned companies that praticipate in tenders by Ministry of Transport and that are interested in providing transportation services in general. We already see a competition between Czech Railways JSC, LEO Express JSC and RegioJet JSC on the main railway route between Prague and Ostrava. This section also explains the distrubution of state aid and the way public railway transport is being ordered. This section also contains a detailed description of the finacial situation of Czech Railways JSC which is currently the most dominant provider of railway transport in the Czech Republic.
|
guest ::
