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Study of fluorescence lifetime and spectral changes of nanoparticles in cell biology
Pelc, Pavel ; Janoušek, Oto (referee) ; Čmiel, Vratislav (advisor)
This work deals with the study of fluorescence lifetime and spectral changes of nanoparticles in cell biology. It describes the principle of fluorescence, fluorescence microscopy and laser confocal microscope Leica TCS SP8. The classic FLIM method, the Lambda Square mapping and the division of nanoparticles are introduced there. In the practical part, the created program for the evaluation of fluorescence lifetime and spectral changes is described. The program can show two-dimensional lambda maps, the fluorescence lifetime and spectral shift in the space area. In the final part of the thesis, an experiment with rhodamine nanoparticles is carried out and it is evaluated using the created program and then discussed.
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The effect of therapeutical ultrasound and metalic nanoparticles on carcinoma cells
Kalužová, Adéla ; Rozman, Jiří (referee) ; Bernard, Vladan (advisor)
This thesis focuses on the research of an effect of ultrasound and nanoparticles on cancer cells. In the literature search there the applications of ultrasound field in the interaction with aplication of metallic nanoparticles to viability of cancer cells are described, on the basis of in vitro experiments‘ performance. The purpose of metallic nanoparticles‘ application should be a possible reinforcing of its antitumor effect thanks to the presence of ultrasound field. In next part of the literature search there are included the technical performance of devices, their indications and contraindications, parameters of ultrasound fields and application of metallic nanoparticles. The metallic nanoparticles have been becoming a part of the human’s life more and more, that is why it’s necessary to deal with these miniature particles at a higher level. The aim of this thesis is to suggest the ways of resolution based on the principle of sounding of cells with the ultrasound field of suitable parameters and in combination with the application of metallic nanoparticles with a successive performance of the cell viability tests. The viability was determinated by the MTT assay test. Another part of this work is the measurement of parameters of this zone by means of a submersible needle hydrophone, for a better understanding of the ultrasonic zone. In the final part of this work there is necessary the statistical evaluation of the measured data of the experiments carried out and the discussion of the results obtained.
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Optical Method to Evaluate the Contractile Function of Isolated Cardiac Myocytes
Kopečný, Josef ; Harabiš, Vratislav (referee) ; Čmiel, Vratislav (advisor)
In this master´s thesis we will firstly focused on the description of a cyte in terms of structure as well as in terms of electrical and chemical processes. We will examine a processes, which make a contraction and the processes on cyte membrane. We will also analyse image processing methods and methods for measuring the contraction. The block diagram will be designed and requirements for the measuring platform will be specified. The programme will be realized in LabWIEV programming language.
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Optical Methods to Evaluate the Contractile Function of Isolated Cardiac Myocytes
Vadkerti, Kristián ; Rychtárik, Milan (referee) ; Čmiel, Vratislav (advisor)
Diploma work is focused on basic characteristics of optical measuring methods commonly used to rate contactions of isolated heart cell and describes basic actions, that are connected with it. A camera record made by a microscope served as basis for optical measuring methods of contractions chosen by us, with using appropriate method elaborating and analysis of pictures used for detection of important part of cell structure. The suggested user application built-up in Matlab environment allows analysis and interpretation of contractional functions in two methodical ways.
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Localization system making use of GPS and GSM
Klozar, Lukáš ; Pust, Radim (referee) ; Šebela, Radek (advisor)
Aim of this master´s project is to analyse localization possibilities in GSM and GPS wireless networks. Choise of proper module and design location device for both GPS and GSM networks. Design and construction of system, which is able to designate it´s positron, using both GPS and GSM networks. The location informations, are send to Internet server, using GPRS system. Localization techniques in both wireless network are described. The system which is able to get position information was designed and constructed. The module´s driving software and the software, which process the localization informations and controls the database storing process, was designed.
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Band Gap - accurate voltage reference
Bubla, Jiří ; Petržela, Jiří (referee) ; Kolka, Zdeněk (advisor)
This diploma thesis is specialized on a design of a high accuracy voltage reference Bandgap. A very low temperature coefficient and output voltage approx. 1,205V are the main features of this circuit. The paper contains a derivation of the Bandgap principle, examples of realizations of the circuits and methods of compensation temperature dependence and manufacture process, design of Brokaw and Gilbert reference, design of a testchip and measurement results.
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Latentní znalosti o buňce u žáků 8. tříd ZŠ a tercie osmiletého gymnázia
STOČESOVÁ, Lucie
This thesis deals with the knowledge testing of the cell biology at pupils in 8th classes and of tercia lower secondary schools by means of the didactic test. Output knowledge of natural history was tested with 200 students (100 boys and 100 girls) in Příbram. The respondents from secondary school showed better knowledge than pupils of the natural history of the 8th classes of the primary school. Results evaluation is presented in words and also illustrated by tables and graphs.
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The Cell Biology - teaching portfolio for educational thematic area of biology for high school
VEJDĚLKOVÁ, Zdeňka
The goal of the diploma thesis is to create a teaching portfolio for educational thematic area of the cell biology. Teaching materials are intended for high school students learning. The portfolio is aimed at the topic of cell construction. The portfolio is projected for the independent work of students. It can be used both in subject matter explanation and as home preparation. A revision test is a part of the portfolio. Activating part of the portfolio is composed of thematic games that are suitable for the subject matter revision and deepening of knowledge.
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Practical exercises in cell biology curriculum for grammar schools
MANDRYSZOVÁ, Jana
This diploma thesis contains a collection of 29 practical exercises, aimed at cell biology. These excercises were developed on analysis of cell biology curriculum in Framework Education Programme for grammar schools, available biology textbooks and professional literature based on this topic. Practical exercises follow up active and inquiry-based approach of students to biology education. The execises include protocol suggestions for exercises, education goals summary, methodical notes for teachers and model answers.
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