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Diagnosis sliding contact using practical applications "Methods particulate"
Kopecký, Jiří ; Mach, Martin (referee) ; Veselka, František (advisor)
This thesis deals with the sliding contact of electrical machines and its diagnostic methods. Namely, the dust-particles method for assessing the length decrease of brushes is deeply described. Then, usage of the method in industry is described. The comparison of accuracy between manual version and new automated version of the method using computer analysis is presented. The final section is devoted to the practical verification of the new automated version which was developed especially for large machines. Measurements were performed on a small machine.
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Study of the influence of magnetic fields on biological tissue
Vlachová Hutová, Eliška ; Fiala, Pavel (referee) ; Bartušek, Karel (advisor)
The aim of this research is to explore the influence of magnetic field on early somatic embryos (ESEs) of spruce and pine. The magnetic field is gradual. This is important for the experiment, each cluster of ESE was exposed to magnetic field of different intensity. The resulting report of this experiment consists of the comparison between various methods of image analysis and the difference in growth of ESEs due to different exposure to magnetic field and surrounding temperature. Enclosed is a proposal of ESE size computing algorithm from a given image.
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Local characterization of electronic devices
Müller, Pavel ; Škarvada, Pavel (referee) ; Tománek, Pavel (advisor)
The development of micro and nanoelectronics and nanophotonics needs novel characterization techniques to ensure higher quality of designed devices. The thesis describes a use of Scanning Near-field Optical Microscopy (SNOM) in dimensional control and in local investigation of diverse physical parameters. As example of its potential, the correlation between object topography and reflection measurement of capacitors is shown.
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Light microscopy and its applications
Pokorný, Pavel ; Prochazka, David (referee) ; Lošťák, Martin (advisor)
This bachelor´s thesis describes problems of light microscopy for readers. Part of this bachelor´s thesis is dedicated to basic terms of the light microscopy. Next chapters are dedicated to observing techniques - brightfield illumination, darkfield illumination, oblique illumination, Rheinberg illumination, phase contrast, differential interference contrast, polarized light microscopy and fluorescence illumination.
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Mobility of photosynthetic proteins
KRAFL, Jaroslav
Mobility of pigment-protein complexes (phycobilizomes and photosystem II playing a key role in photosynthesis) was studied by FRAP method (Fluorescence Recovery After Photobleaching). FRAP represents a fluorescence based microscopy method enabling measurement of protein mobility in living systems. The protein complexes are bleached by a laser pulse. And mobility of unbleached proteins is measured as a fluorescence recovery in the bleached area. Currently we have only limited knowledge about the mobility of photosynthetic proteins. This work was aimed at optimization of the photosynthetic protein mobility measurement by FRAP. I have performed several methodological experiments which led to the successful assessment of phycobilisome and chlorophyll-containing proteins diffusion coefficients in selected red algae (Porfyridium cruentum, Cyanidium caldarium) and cyanobacteria (Synechocystis PCC6803, Acaryochloris marina). The methodology developed and validated in my thesis was then applied in further research projects.
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Microscoping measurements in physical practicum
TISOŇ, Václav
This bachelor work is considered description of on-line measurement Motic Image plus 2.0, which isn´t only acceptable optical device for school practical measurement, but it´s very useful for optical measurement of very thin objects with microscope. The user can due to a simple program see very strict proportions in milimeters order distances. This program is suitable almost for everyone and the operating is very easy.
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Physical principles of transmission and scanning electron microscopy
BÍLÝ, Tomáš
This bachelor thesis focuses on the description of transmission and scanning electron microscopy. It describes physical principles and defects arising during image formation in electron microscopes as well as the methodology of adjustment. The basic parts of electron microscopes are looked into more closely herein with consideration to the lectures. Practical implementation is demonstrated on following microscopes JEOL: JEM-1010, JSM-7401F, JDM-6300.
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