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Audit informačního systému společnosti Terms a.s.
Eremiáš, Karel ; Svatá, Vlasta (advisor) ; Maule, Pavel (referee)
The work summary problems of information systems audit and use these findigs while performing information system audit in a real company. Audit is performed using COBIT methodology and it respects not only requirements set by methodology but even special requirements set by organization. The work is also checking usability of COBIT methodology while performing audit in the conditions of medium size czech company.
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Method of Voltage Contrast in ESEM
Buchta, Michal ; Černoch, Pavel (referee) ; Jirák, Josef (advisor)
This graduation thesis deals with the problem of voltage contrast in ESEM. The purpose of this work was to verify influence of used detectors in the dependence on conditions in specimen chamber on the size of voltage contrast. With the conditions in specimen chamber we understand pressure and working conditions of signal detection. We used power transistor as specimen.
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Characterization of specific proteins form selected animal products.
Janhuba, Filip ; Obruča, Stanislav (referee) ; Márová, Ivana (advisor)
The master's thesis is focused on study of specific protective proteins from animal products. Two different types of antimicrobial egg white proteins were studied in detail - antimicrobial protein ovotransferrin (conalbumin) and enzyme lysozyme. Ovotransferrin belongs to transferrin group of proteins and exhibits activities similar to milk protective protein lactoferrin. The main effects of ovotransferrin are antiviral, anticancer and immunomodulatory. Antimicrobial activity of ovotransferrin based on the possibility to bind iron is still a subject of interest. For comparison the second egg protein lysozyme (N-acetyl muramidglycan hydrolase) was used. Lysozyme is a hydrolytic enzyme which primary attack cell wall of bacteria. In the theoretical part of the thesis an overview of the specific antimicrobial proteins in selected animal products was introduced mainly focused on ovotransferrin and lysozyme. The experimental part of this work was focused on optimization of methods for the determination of antimicrobial activity, protein concentration and purity. For quantitative analysis of total proteins, optimized Hartree – Lowry spectrophotometric method was used. For the determination of molecular weight and purity SDS-PAGE was used and stained by Coomassie Brilliant Blue G250 and silver. In experimental part the real sample of egg white was compared with samples of lyophilized antimicrobial proteins and therapeutical pills supplied by industrial partner. Protein composition and purity of these preparative has been determined. Antimicrobial activity of ovotransferrin was studied on cultures of G+ bacterium Bacillus subtilis and for comparison on G– E. coli. Ovotransferrin showed antimicrobial effect only at very high concentrations of about 75 mg/ml (Bacillus subtilis) and 50 mg/ml (E coli) even with addition of high amount (100 mM) of hydrogen carbonate ions. The inhibitory effect was most evident in liquid media. On the other hand, lysozyme exhibited significant inhibitory activity from 0.3 mg/ml on gram positive bacteria. Inhibitory effect on E. coli was not observed. Another part of study was focused on isolation of ovotransferrin from egg white using gel permeation chromatography on Sephadex G100. As mobile phases 0.1 M phosphate buffer and 0.05 M Tris-HCl buffer were tested. By SDS-PAGE the purity of ovotransferin comparing to standard was evaluated. Finally, the encapsulation of ovotransferrin and lysozyme was tested. Ovotransferrin and lysozyme was encapsulated into liposome and chitosan particles. Particles stability, distribution and average size distribution were studied by dynamic light scattering and zeta potential measurement. The stability of particles in the model physiological conditions was studied too.
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Technology Solution for Valve Body Components
Vávrová, Monika ; Osička, Karel (referee) ; Kalivoda, Milan (advisor)
The solution bachelor work is the elaboration of a production piece "valve body" in two variants in terms of medium-sized companies. Work is focused on the analysis of the given part, on the choice of machine options the company on the production process a given set of tools and the design of new technologies. Finally, the technical and economic evaluation of the technology in the production of both variants.
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Antimicrobial peptides and their application into foods
Janhuba, Filip ; Petrik, Siniša (referee) ; Márová, Ivana (advisor)
The presented thesis is focused on a study of antimicrobial peptides and their potential applications into foods. Two types of antimicrobial peptides were studied in detail - bacteriocin nisin and antimicrobial enzyme lysozyme. In theoretical part an overview of antimicrobial peptides used in food industry was introduced. The experimental part of this work was focused on optimization of methods for the determination of antimicrobial peptides concentration. Commonly used spectrophotometric methods for quantitative analysis of total proteins as well as high performance liquid chromatography were tested. To determine the molecular weight and purity a modified SDS-PAGE with the addition of urea and tricine buffer was performed. Further, encapsulation of nisin into liposome was tested. Encapsulated particles were prepared by three different procedures: by ultrasonication, ethanol injection and TLE. Particles stability, distribution and size were studied by dynamic light scattering. Stability of encapsulated peptides in model conditions and model foods was studied too. Finally, antibacterial properties of the selected peptides using test system Bacillus subtilis were studied. Nisin already showed antimicrobial effect at low concentrations - about 1 µg/ml. The inhibitory effect of nisin was more evident in liquid cultures.
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Piston for 43kW 4-stroke SI-engine
Vaculík, Tomáš ; Svída, David (referee) ; Dundálek, Radim (advisor)
This bachelor’s work describes a calculational project and design solution of a piston for 43 kW power output, four-stroke petrol engine and depicts basic sizes of this engine. The piston was constructed to be able to work in hard conditions that the piston faces in the combustion area. An important part of my thesis is the piston calculation of its resistancy by which we verify corectness of suggested sizes of the piston.
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Analysis of variance in R
Švejdová, Klára ; Matějka, Martin (advisor) ; Sobíšek, Lukáš (referee)
The bachelor thesis focuses on the One-way ANOVA and its nonparametric counterpart the Kruskal-Wallis test. The aim of this bachelor thesis is to analyse the ANOVA's test validity and power in case of assumptions (e. g. normal distribution, homoscedasticity and sample size) violations. The conclusions of the One-way ANOVA are compared with the Kruskal-Wallis test. The analysis is performed using Monte Carlo simulations implemented in the statistical software R. Based on the simulations, it is found out that the One-Way ANOVA is not significantly sensitive to the violation of normality, if the homoscedasticity assumption is met within each of four samples. On the other hand it is found out that the One-Way ANOVA is significantly sensitive to the violation of homoscedasticity at the particular specification. The Kruskal-Wallis test yields in better results if the homoscedasticity assumption is violated. In case that the One-Way ANOVA and the Kruskal-Wallis test are valid, the ANOVA provides higher power of the test.
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Male Sterlet (Acepenser ruthenus) Fertility under Fish Farming Conditions of the Czech Republic
SCHACHERLOVÁ, Jiřina
Currently, fish fertility studies are mainly focused on female fertility. Studies showing fertility of male fish of any kind are missing to a large extent. The main objective of my thesis was to identify what level of fertility is achieved by male sterlet (Acipenser ruthenus) in the conditions of fish farming in the Czech Republic. Male fish from the Vodňany farm where the raised population comes from Russia was compared to male fish from Pohořelice where the raised population comes from Danube. The total comparison test involved 49 pieces of fish. Following stripping to collect semen from male fish, the concentration of sperm (.109.ml-1) was measured in a 1 ml-1 semen sample. Consequently, a sperm count per 1 kg-1 male fish (.109.ml-1) and a sperm volume per 1 kg-1 male fish (ml-1) and a total male fish sperm count (.109) were measured. The highest values of weight identified in male fish was measured in the Pohořelice fish farm. The lowest values of weight was measured in male sterlet of the Vodňany fish farm. The highest as well as lowest volumes of collected semen per 1 kg-1 male fish were identified in Vodňany. However, the average volumes of both cultures were not significantly different. It was concluded by comparison that collected semena volume does not depend on the weight of said fish. Also, the relative fertility was significantly different between male fish of the same population. The highest and lowest sperm count per 1 kg-1 male sterlet were identified in Vodňany. The average minimum and maximum sperm counts per 1 kg-1 male fish were different very much which shows that a greater number of male fish should be used for spawn to ensure sufficient egg fertilization. The average overall fertility values in both cultures are very similar and mainly different in specific specimen of the same culture as well as between those cultures. The results showed that a total sperm count in semen depends on the volume of collected semen. It was further concluded that sterlet fertility does not depend on the size (weight) of fish.
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Reprodukce a intenzivní chov juvenilních ryb štiky obecné (Esox lucius L.)
BONDARENKO, Volodymyr
Fish hatcheries do not always provide optimal conditions for controlled reproduction and production of high-quality juvenile in northern pike. This study was undertaken to optimize reproduction and intensive juvenile culture of northern pike (Esox lucius L.) under controlled conditions. The efficacy of traditional treatments for induction and synchronization of ovulation in northern pike (carp pituitary or ambient outdoor conditions) was compared to that of sGnRH-a at 50 and 100 ?g kg-1 with or without Freund´s incomplete adjuvant and dopamine inhibitor (metoclopramide, 8 mg kg-1). Ovulation was observed in females treated with carp pituitary (3 mg kg-1) and in those held in ambient conditions. Sperm motility parameters were compared in northern pike and sterlet (Acipenser ruthenus Brandt). Analysis was accomplished via high speed video microscopy complemented with CASA analysis. This work described recently developed methods of recognizing and quantifying sperm quality. The data obtained will lead to more accurate modeling of flagellum behavior during the motility period and provide a deeper understanding of basis of spermatozoa motility. The effect of water temperature on duration of embryo ontogenesis and hatching period; fertilization and development to the gastrula stage; hatching rates; and quality of larvae including larval size, development, and resistance to osmotic stress under controlled conditions for northern pike were investigated. Results indicated that the optimal temperature range for northern pike embryo development under controlled conditions is 6-10oC. Northern pike embryonic development hypothetically ceases at approximately 3.3 °C. In the certified methodology the basic aspects of controlled reproduction of northern pike was described and explained, including optimization of broodstock management. An objective of the certified methodology was to describe methods for producing high quality juveniles.
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Reproduction Burbot burbot (\kur{Lota lota}) and incubation of eggs at different temperatures in service
MIKEŠOVÁ, Lucie
The study evaluated the reproductive performance in Burbot burbot (Lota lota L.) and compared the incubation period at two locations. It was confirmed that the burbot wipes at the end of December and beginning of January with an average temperature of 1,93 ? 0,8 ° C. It was found that the content of the eggs in one gram is 3325 ? 248 pieces eggs and 1 ml is 1434 ? 24 pieces of eggs. Also, to evaluate the size of eggs before and after swelling. Size before swelling was 0,814 ? 8 mm and the swelling 0,838 ? 7,1 mm. Further, the absolute and relative fecundity and GSI, the absolute fecundity was 289,279 ? 204,759 pieces of eggs, the relative fertility of 763,523 ? 110,061 pieces of eggs per kilogram of body weight of fish and GSI was 23,15 ? 3.9%. The last parameter was rated incubation period. The incubation period was Vodňany 178,4 ° D and Borových Ladách 145,4 ° D.
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