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Use of autotrophic algae to production of biomass and some metabolites
Chrástová, Nikola ; Kostovová, Iveta (referee) ; Márová, Ivana (advisor)
Chlorophyll and carotenoids are natural pigments and antioxidants. These pigments are suitable for use in the pharmaceutical, cosmetics or food industries. Lipids are another major metabolite of algae and are a potential feedstock for biofuel production. This bachelor thesis deals with the cultivation of selected genera of autotrophic algae, subsequent isolation of these selected metabolites and their application. The theoretical part focuses on the description of the algae, the metabolites produced, the cultivation and the chosen methods. The experimental part deals with the optimization of the cultivation of algae of the genera Chlorella, Chlamydomonas and Desmodesmus, depending on the chosen medium and intensity of illumination, determination and quantification of algal biomass. Further, extraction of lipidic and carotenoid substances and determination by HPLC and GC was performed. In the overall comparison, the best producer of biomass is the algal genus Desmodesmus and the highest production of pigments and lipid substances was reached in algae of the genus Chlamydomonas.
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Vegetable adaptogens - Schizandra chinensis
Pospíchalová, Lucie ; Půčková, Helena (referee) ; Kotlík, Josef (advisor)
This thesis deals with plant adaptogens (bioactive substances) in the plant Schisandra chinensis. The theoretical part is focused on characterization of the Schisandra genus, characterization of the Schisandra chinensis as a plant, its properties, occurrence and growing possibilities in the regional climate zone of the South Moravian Region as well as the use of this plant. Furthermore, adaptogens are described from a chemical point of view and their possible methods of isolation from individual parts of plants. The experimental part deals with extraction of fruits and leaves from plant Schisandra chinensis. Further, in the leaves extract was determined chlorophyll a, chlorophyll b and carotenoids by spectrophotometry, where the quantity of individual substances was confirmed for months April – May.
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Determination of Chlorophyll Content in Birch and Pine Trees Using Hyperspectral Data
Zachová, Kateřina ; Potůčková, Markéta (advisor) ; Brodský, Lukáš (referee)
The master thesis deals with the determination of the chlorophyll content in birch foliage (Betula pendula Roth) and Scots pine using hyperspectral data. The first part of the thesis concentrates on the literature search dealing with the methods of chlorophyll content in the foliage of selected plant species. In the practical part the emphasis is on the study of spectral reflectance curves and finding their relation to the chlorophyll content from the laboratory determination. Images taken with the hyperspectral sensor HyMap and spectral reflectance curves obtained with the ground ASD FieldSpec 3 spectrometer were available. Using the derived regression model chlorophyll maps were created for Scots pine for three selected locations in the Sokolov coal basin area.
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Response of different genotypes of Zea mays L. and Vicia faba L. treated with brassinosteroids to water deficit
Kuklíková, Radka ; Rothová, Olga (advisor) ; Hnilička, František (referee)
This work monitors the response of plants to the application of a solution of synthetic brassinosteroid (5-fluor-3α,17β-dihydroxy-5α-pregnan-6-on) under drought stress conditions and subsequent restoration of water availability. The response of two plant species was monitored under six-day period of cessation of watering and under six-day period of renewed optimal water supply. The experiments were carried out on plants with different type of photosynthesis - C3 plant Vicia faba L. and C4 plant Zea mays L.. For both plant species, three varieties/genotypes were used. Photosynthetic, morphological and developmental characteristics were monitored. The brassinosteroid treatment influenced more the morphological than the photosynthetic characteristics. The content of photosynthetic pigments was influenced more than the chlorophyll fluorescence parameters. The differences in morphological characteristics between individual varieties/genotypes were observed. The response to the application of brassinosteroid was observed especially for drought-sensitive variety/genotype. Application of brassinosteroid caused increase of the dry mass of leaves and roots, the height to leaf , the height of the whole plant and its increment under stress and post-stress periods in both plant species. The content of...
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Inter- and intraspecific variability in Vicia faba L. and Zea mays L. response to water deficit
Fridrichová, Lenka ; Holá, Dana (advisor) ; Zelenková, Sylva (referee)
This work monitors the response of plants to water deficit and subsequent restoration of water availability with the aim to expand our knowledge about plant behaviour during and after drought stress from the perspective of two plant species with a different type of photosynthesis. A particular attention was paid to the intraspecific differences and their possible changes during stress and post-stress periods. The plant response was monitored as the changes of selected photosynthetic, morphological and developmental characteristics caused by the cessation of watering for six days followed by a six-day period of renewed optimal water supply. The experiments were carried out on plants with different types of photosynthesis - C3 plant Vicia faba L and C4 plant Zea mays L.; for both plant species, three varieties / genotypes were monitored. The response of both plant species to insufficient water supply differed by the extent of changes in relative water content (RWC), chlorophyll fluorescence parameters, the dry mass of individual leaves (developed during the stress period), the ratio between the aboveground dry mass and dry mass of roots. In drought-stressed plants, downward trend in RWC was observed, as well as the increase of the minimum yield of chlorophyll fluorescence in dark-adapted leaves (F ),...
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Effect of different treatments of corn to increase biomass production
Pokorný, František ; Tomášek, Jaroslav (advisor) ; Ondřej, Ondřej (referee)
Maize is one of the most profitable crops in our country. Therefore, new technological processes are implemented and developed at growing maize. Despite the relatively extensive knowledge and experience in growing maize, these technologies are abundant with risks and possible pitfalls. A very important question above all is the one concerning the soil treatment; fertilization and nutrition suitable for maize growing. Thesis deals with proposing an appropriate system of fertilization the maize suitable for silage by all different means of wide fertilization applications, fertilization supplements at the base and at the top with relation to production of silage. During the vegetation, we took measurement of maize plants. Thesis also evaluates the intensity and volume of chlorophyll in leaves of maize that was not treated. The experimental plants of maize were situated on testing field of Research Station, Faculty of Agronomy. Food and Naturall Resources at the Czech Agricultural University in Prague in Červený Újezd (district Prague - West). After the harvest, all variants of maize were examined from the perspective of profit in green state and in the dry state. Within the one-year experiment, we found following results that may help in the planning the basic or supplemental fertilization of silage maize. Therefore, based on the results launched, we present that the wide top use of Zeastim fertilizers in combination with Urea Stabil fertilizer has impact on higher growth of green mass, so it proves higher profit of green and dried mass.
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Sledování intenzity fotosyntézy u skleníkové kultury zeleniny
Konvičná, Petra
The literary part of the work approaches the plant material, cucumbers (Cucumis sativus L.), the issue of greenhouse production and growing in hydroponics. A large part is devoted to describing the process of photosynthesis and the different factors affecting the impact and progress of the process. The practical part is devoted to monitoring and measuring the physiological characteristics of the photosynthetic apparatus using LCpro +: the intensity of photosynthesis, transpiration levels, water efficiency, stomatal conductance with cucumbers. Outside the parameters of photosynthesis is also evaluated chlorophyll content using the device CCM200plus Chlorophyll Content Meter and the ability of plants to use sunlight, thus fluorescence. Flourescence was measured with a fluorometer OS30p + Chlorophyll. Based on the evaluation, it was found that all measured characteristics were influenced by physiological and health status and degree of leaf damage.
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Investigation of the impact of stress factors on structure and function of the photosynthetic apparatus of rape (/Brassica napus /L.
RÁKOSNÍKOVÁ, Tamara
The thesis deals with the influence of cold plasma,as a stress factor, on content and fluorescence of chlorrophylls of oilseed rape. Theoretical part deals with characteristic oilseeds rape, fluorescence, photosynthetic pigments and the effects of cold plasma on on physiology of plants. The theoretical part continues in experimental mesurement. The eperiment was done in laboratory conditions. The own of eperiment was to discover the influence of cold plasma on the amount of chlorophylls and fluorescence of chlorophylls. The rape seads were treated with cold plasma for the duration of various time interval. The thesis considersif the plants, which were sprouted from treated seeds, show higher values of physiological activities as compared with plants germinated from conventional seed.
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