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The influence of acyclic nucleotide phosphonates PMEG and PMEDAP on p38 kinase signaling in human leukemic cells
Nejedlá, Michaela ; Entlicher, Gustav (advisor) ; Slaninová, Jiřina (referee)
PMEG [9-(2-phosphonomethoxyethyl)guanine] and PMEDAP [9-phosphonomethoxy- ethyl)-2,6-diaminopurine] are acyclic nucleoside phosphonates possessing cytotoxic properties. Antiproliferative effect of PMEG was demonstrated in various tumor cell lines in vitro. PMEG also represents an active component of some experimental prodrugs with enhanced selectivity and efficacy (such as GS-9219). PMEDAP seems to have weaker effect in vitro compared to PMEG, however it exhibited pronounced antitumor effect in SD-rats with spontaneous lymphoma. Therefore it was included in the present study as well. The aim of this study was to describe the interactions of PMEG and PMEDAP with p38 MAP kinase signaling and its relationship to the apoptosis. We investigated the influence of these compounds on the expression of four genes encoding p38 MAPK isoforms and whether this change is translated into the protein. It was found that PMEG up-regulates p38β and γ mRNA in CCRF-CEM cells and p38 β and δ in HL-60 cells. The effect of PMEDAP was less pronounced than that of PMEG. However, total p38 protein level remained unaffected by PMEG and PMEDAP. Activation of p38 MAPK cascade was also measured in the cells exposed to these agents using phospho-specific antibodies. We found that neither PMEG nor PMEDAP activated p38 kinase...
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Studying of atorvastatin effects on atherogenesis in apoE-/-/LDLR-/- double knock-out mouse model of atherosclerosis II.
Zajíčková, Jana ; Nachtigal, Petr (advisor) ; Štaud, František (referee)
Atherosclerosis is a degenerative disease of blood-vessel wall, which attacks all population. It is very widespreaded and nowadays it is also one of the most frequent causes of death in the all economically advanced countries. In recent years the extensive research of atherosclerosis have brought a number of new pieces of knowledges, which have contributed to understanding of actions that happen during the process of atherogenesis. ApoE/LDL - receptor double knockout mice represent an animal model for study of atherogenesis, which is able to evolve severe hyperlipidaemia and atherosclerosis. Statins are currently the most considerable and the most useful hypolipidemic drugs. They significantly decrease levels of total cholesterol and LDL cholesterol and also decrease mortality and morbidity of cardiovascular diseases. The aim of the diploma thesis was to find out and describe the expression of SMAD 2/3 in atherosclerotic plaques in apoE/LDL-receptor deficient mice and to study possible atorvastatin effects on its expression. The female mice at the age of eight weeks were used in the study. Animals were divided �into� two �groups. The �control� group �of �animals� was fed with the western type diet.����same atherogenic diet was�used in��atorvastatin group,�where�atorvastatin�was�added to the...
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Studying of atorvastatin effects on atherogenesis in apoE-/-/LDLR-/- double knock-out mouse model of atherosclerosis I.
Stráský, Zbyněk ; Nachtigal, Petr (advisor) ; Štěrba, Martin (referee)
Atherosclerosis, or sclerosis of arteries, is a degenerative disease of arteries. Sometimes it is called "the disease of 20th century". ApoE/LDL - receptor double knockout mice represent a new animal model for study of atherogenesis, which is characterized by severe hyperlipidaemia and atherosclerosis. Statins (or competitive inhibitors 3-hydroxyl-3-methyl-glutaryl-coenzym Areductase) currently belong to the most efficient and the most useful hypolipidemic drugs for all over the world. They decrease mainly levels of total cholesterol and LDL cholesterol. The aim of this thesis was to describe the expression of endoglin in atherosclerotic plaques in apoE/LDL-receptor deficient mice. Moreover we wanted to determine the effect of atorvastatin treatment on the expression of both endoglin.. ApoE/LDLR-deficient mice on were subdivided into 2 groups. The control group of animals was fed with the western type diet. The same atherogenic diet was used in ATV group, where atorvastatin was added to the atherogenic diet at the dosage of 100 mg/kg per day. The results of this thesis confirmed the expression of endoglin in atherosclerotic lesions in ApoE/LDLR-deficient mice. The expression of endoglin was located on the aortic vascular endothelium and in other smaller vessels and capillaries of surrounding...
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Studying of atorvastatin effects lipid profile in mice models of atherosclerosis.
Slanařová, Martina ; Nachtigal, Petr (advisor) ; Hyšpler, Radomír (referee)
Atherosclerosis is a chronic inflammatory disease of blood vessels with a successive, very slow progress which takes many years and ,,plugs"disabled vessels. Faults and changes in representation and quantity of lipoproteins are for this disease very typical. In human medicine is mostly used medication Statins during a therapy of dyslipoproteinemia. The aim is this thesis was to describe the changes of biochemical lipoproteins levels at the various mice models of atherosclerosis. Furthermore we wanted to describe the changes lipids after administering of atorvastatin in apoE-defitient mice and apoE/LDLr-deficient mice. Female of apoE-deficient mice and apoE/LDLr-deficient mice were divided into a control and with atorvastatin treated group, where atorvastatin was administered in a period of 8 weeks in a dose of 100 mg/kg mouse. Biochemical analysis of lipids was performed by a separation method of lipoproteins by means of ultracentrifugal technique. Biochemical analysis of blood of apoE-deficient mice demonstrated, that 8 weeks atorvastatin treatment in a dose of 100 mg/kg significantly increased levels of total cholesterol, VLDL, LDL and HDL cholesterol. The blood analysis of apoE/LDLr-deficient mice demonstrated, that 8 weeks long treatment with atorvastatin in the same doses significantly reduced...
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Dynamic hash tables
Vitovják, Radek ; Koubek, Václav (referee) ; Koubková, Alena (advisor)
The aim of the work is to describe various methods allowing the change of an internal hash table size in dependence on the number of inserted records and to compare them on the basis of known theoretical results. Then to make an experimental study of performance and mutual comparison of chosen methods on simulated data. To compare the results with theoretical findings and published precedent results, if any exist. The first part of the work describes implementation of hash tables and the analysis of expected number of key comparison for a successful and unsuccessful search. The next part contains experimental results of the performance of hash tables implemented on the basis of description stated in the previous part.
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Monitoring expression of selected cytokines in murine models of atherosclerosis.
Tlapalová, Barbora ; Nachtigal, Petr (advisor) ; Štaud, František (referee)
Atherosclerosis, or sclerosis of arteries, is a degenerative disease of arteries. Sometimes it is called "the disease of 20th century". ApoE/LDL - receptor double knockout mice represent a new animal model for study of atherogenesis, which is characterized by severe hyperlipidaemia and atherosclerosis. Statins (or competitive inhibitors 3-hydroxyl-3-methyl-glutaryl-coenzym Areductase) currently belong to the most efficient and the most useful hypolipidemic drugs for all over the world. They decrease mainly levels of total cholesterol and LDL cholesterol. The aim of this rigorous thesis was to describe the expression of endoglin and SMAD 2 in atherosclerotic plaques in apoE/LDL-receptor deficient mice. Moreover we wanted to determine the effect of atorvastatin treatment on the expression of both endoglin and SMAD 2. ApoE/LDLR-deficient mice on were subdivided into 2 groups. The control group of animals was fed with the western type diet. The same atherogenic diet was used in ATV group, where atorvastatin was added to the atherogenic diet at the dosage of 100 mg/kg per day. The results of this thesis confirmed the expression of endoglin and SMAD 2 in atherosclerotic lesions in ApoE/LDLR-deficient mice. The expression of endoglin was located on the aortic vascular endothelium and in other smaller...
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Questions and interrogative sentences in Czech
Dřímal, Jakub ; Hošnová, Eva (referee) ; Nebeská, Iva (advisor)
The aim of this thesis is to describe the system of questions and question sentences in Czech language. The question is defined here as being an utterance with interrogative function. In the first chapter, the position of question within the system of other communicative functions is being searched for. The primary function of questions (interrogative) and distinctive formal devices of question sentences (e.g. rising tone in a yes/no question or a question-word in a wh-question) can be contrasted with secondary functions, e.g. an expression of an expectation of a certain answer, surprise etc. Those can be signalized by the form, for instance by the word order, negation or by particles. Complex relations between both primary and secondary formal devices of questions and their functions are examined. The questions without interrogative form and the question sentences lacking interrogative function are dealt with too. In the second part of the thesis, the theoretical observations are applied in the analysis of the questions in TV debates. Therefore, the interpretation is extended with the information about the specific forms and use of interrogative utterances in an authentic dialogue.
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For- social behaviour of nurses
KOCMICHOVÁ, Kateřina
This work is focusing on questions of for-social behavior and altruism of nurses. For-social behavior is an important social phenomenon, such an antipole of non-provoked agresivity and probably of all other bad behavior (asocial, antisocial) . It is such behavior that keeps social bindings and that makes us closer one another. For-social behavior is characterized by acts done in favor of others without expectation of reward (financial, material) or social approval. In the first part of the diploma paper, there is outlined question of for-social behavior at theoretical level. Here is defined the term of for-social behavior, altruism, empathy with effort to answer the question why we help others, under what conditions, circumstances and to whom. The intention of this work consists in exploration-description of for-social behavior of nurses. The work deals with scale of empathy, altruism and affiliation of nurses in comparison with women non-working in helping professions. Furthermore, there is investigated the ability of nurses to cope with stress and that is measured by means of optimism scale. I did four hypotheses, all of which proved to be right. I used a method of questionnaire in terms of quantified research. Each hypothesis was based on its own separate questionnaire in question form. It was 200 question forms in total, 100 forms for nurses and the same for women non-working in helping professions as a control group. 174 filled forms were given back. The basic group was formed by 88 nurses and the control group by 86 non-nurses. The outcome of questionnaire research is that nurses show higher measure of empathy, altruism and affiliation than women non-working in helping professions. The nurses were also proved to be less adaptable to stress than non-nurses.
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Employee selection, appraisal and development by means of the assessment centre
Dočekalová, Klára ; Michalová, Lucie (referee) ; Šnýdrová, Ivana (advisor)
The aim of this study is to clarify that is the Assessment Centre method, it's theoretical background and to focus on psychodiagnostic methods and their suitability for usage in Assessment Centre. Close attention is paid to expected future for this method. First part of this study is focused on definition of the method, usage areas and theoretical background. Measuring for the method effectiveness is important for standardization procedure. For that reason is study aimed on criterias that needs to be measured. The main attention is paid to diagnostic tools used in Assessment Centre. The final part is focused on program realization process.
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Burnout military nurses versus civil nurses
Dušková, Klára ; Rybářová, Marie (advisor) ; Býma, Svatopluk (referee) ; Jirkovský, Daniel (referee)
This bachelor thesis deals with burnout syndrome. Theoretical part of this work brings the introduction to the problems and examples of persons threatened by this syndrome, description of the symptomes and the course of burnout syndrome. Further, the work brings information on potential diagnostics, prevention and interventions. Practical part maps, which group of the addressed nurses is exposed to higher risk of burnout syndrome inception, whether military or civil nurses and if the total practice time spent in health service influences the burnout syndrome inception. Analysis and evaluation follows of the burnout syndrome manifestations ascertained at the above mentioned groups, comparison and statement which group of the addressed nurses faces higher risk of burnout syndrome.
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