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LINC complex: The link between chromatin integrity and sperm motility
Šanovec, Ondřej ; Komrsková, Kateřina (advisor) ; Lánská, Eva (referee)
The LINC complex (Linker of the Nucleoskeleton and Cytoskeleton) is a protein structure located in the nuclear membrane that connects the cytoskeleton with the nucleoskeleton. This complex can be found in every mammalian cell including the gametes. However, here the LINC complex is more diverse and less studied than in the somatic cells. In this thesis, the LINC complex and its role in spermiogenesis have been studied in wild-type and Protamine 2 knockout (Prm2-/- ) mice. Protamines are small proteins that replace histones during spermiogenesis. The mouse model generated by the group of prof. Hubert Schorle has a deletion in Prm2 in exon 1 and its sperm possess a surprising phenotype including complete loss of motility. Therefore, it was hypothesized that the LINC complex might be responsible for miscommunication between the sperm head and tail which leads to the loss of sperm motility. Results from this study suggest that the LINC complex is not influenced by Prm2 deletion, however, actin dynamics, cytoskeletal motor proteins and tubulin acetylase/ histone deacetylase activity might be impaired. Prm2-/- sperm have a significantly higher abundance of β-actin compared to the wild type. Next, Prm2-/- sperm also show a different pattern of acetylation of α-tubulin but no change in the abundance of...
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Physical and mental performance of morning and evening chronotypes in different times of day
Červená, Kateřina ; Bendová, Zdeňka (advisor) ; Jelínková, Dana (referee) ; Větrovský, Tomáš (referee)
Circadian rhythmicity, which evolved in accordance with the rhythmicity of the external environment tied to the earth's rotation, governs the regular rhythms of our physiology and behavior. The central circadian clock in the suprachiasmatic nuclei of the hypothalamus controls the roughly 24-hour rhythmicity of many peripheral clocks of most cells of the body, that can be observed from the molecular to the behavioral level. The intrinsic circadian period (τ) in humans is on average slightly longer than 24 h, but may also be slightly shorter. Individuals with a short τ are referred to as morning chronotypes, whereas individuals with a long τ are referred to as evening chronotypes. However, the majority of the population belongs to the so-called intermediate chronotype. The molecular mechanism of circadian oscillations controlled by the circadian clock has been shown to be closely linked to cellular metabolism. Many physiological processes important for physical and cognitive performance are also regulated by the circadian system. The main aim of both studies was to quantify the difference in performance during morning and evening testing in subjects of a pronounced evening and morning chronotype. While the motivation for conducting Study A was practical, with the aim of identifying potential biases...
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Protists in oral cavity of humans and animals.
Brixí, Kateřina ; Tachezy, Jan (advisor) ; Rada, Petr (referee)
Trichomonas tenax, Trichomonas gallinae, Trichomonas gypaetinii, Trichomonas stableri, Trichomonas equibuccalis, Trichomonas brixi, Tetratrichomonas canistomae, Tetratrichomonas felistomae and Tetratrichomonas empyemagena are unicellular eukaryotes (protists) that may occur in oral cavity of humans and domestic animals. These protozoans are mostly referred as commensals and relatively low attention is paid to them, since they usually are not primary cause of serious health problems. However, based on current knowledge, it can be concluded that more appropriate term for Entamoeba gingivalis and Trichomonas tenax would be an opportunistic pathogen, while Trichomonas gallinae is a primary pathogen. There are very little studies and findings about the rest of oral protozoans and their pathogenicity, therefore it is not possible to assess their significance for a host. Nevertheless, the oral protozoans should not be neglected, as in some cases they can cause serious health complications in their host.
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Info(VR)architecture
Ponomarevová, Daniela ; Janoščík,, Václav (referee) ; Mikyta, Svätopluk (advisor)
In my bachelor thesis, which I called Info(VR)architecture, I base on my previous projects, in which I have developed through symbolic-dystopian narratives a reflection about human mortality and the fear of losing control, and the influence and power of information of the internet on the state of our shared reality. I deconstruct and reunite these into new constellations in which I explore the relationship between virtual and physical reality and the human being.
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How will we live together?
Wintner, Roman ; Kocián, Václav (referee) ; Kratochvíl, Jan (advisor)
In my thesis How We Will Live Together I deal with the phenomenon of participatory housing. The first phase of the thesis is a research in which I analyse types of community housing from abroad, I examine the history of collective housing in the Czech Republic. I focus on different possibilities of participation within housing, which I demonstrate with real examples. I try to point out the still insufficient support of similarly oriented projects that could have a positive impact on the development of the housing issue in the country. In the second phase of the thesis, I develop a characterization of a fictive group of people, a community, on which I try to demonstrate the functioning and feasibility of a participatory housing project and the bottom-up model. The result is an architectural design on the site of the former sugar factory in Šlapanice, taking into account the interests and needs of the site and the community. It is a kind of reflection pointing out the possibilities and variability of the implementation of such a project in specific Czech conditions. I am basing my ideas on a foreign model, which I am trying to transfer to the territory of a more relaxed development. An important consideration in the work is given to the questions of the future in connection with housing. In the project I am working with the design of 5 different buildings, one of which also serves as a common centre for the community. The buildings are characterised by different approaches to design according to the individuality of the group of owners. I bring further considerations of possible functioning through the different zoning of the plots without strict subdivision and the different approach to the use of cars. As a paradoxical response of future housing, I bring a return to tradition and customs similar to the village environment. Not only in connection with cultivation, poultry farming, escape from hecticness, but especially with an orientation towards real values and healthy relationships, which can be mediated precisely by participatory housing.
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Perception of the human disturbance by ungulates in the wild and in the captivity
Šťastná, Tereza ; Komárková, Martina (advisor) ; Frýdlová, Petra (referee)
The relationship between ungulates and humans has been evolving ever since the first predator's interaction with the prey, leading to the current domestication. The behavior of ungulates differs based on the human disturbance. The goal of this thesis was to compare the reactivity to humans of selected species in nature vs. in captivity and determine its correlation, inter or intraspecific. The thesis indicates reactivity to humans of specific species of ungulates (donkeys, horses, reindeers and goats). Following reactions were chosen for comparison: vigilance, alert distance, flight and avoidance distance. There are several acting factors like previous experience with humans, habituation, group size or season. With group size, the vigilance decreased, which was proven with donkeys, reindeers and goats. The behavior differed based on the season by resources availability, offspring's presence in herd (higher vigilance) or rut (lower vigilance). In captivity, there were less human contact avoidance, while the experimenter was known. Based on researches, ungulates tend to avoid humans more in nature, than in captivity. Sources claim, that among chosen ungulates, the most vigilant in both nature and captivity are reindeers. Further researches could later consider the human perception according to the...
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The Topic of the Death in Existential Philosophy and its Influence on the Theological Reflection
Kuntová, Karolína ; Vogel, Jiří (advisor) ; Kučera, Zdeněk (referee)
Diploma thesis "The Topic of the Death in Existential Philosophy and its Influence on the Theological Reflection" deals with the Death in work of existential philosophers as Martin Heidegger, Jean - Paul Sartre, Karl Jaspers and Albert Camus. Authors concentrate on human being, which is direct to the Death. They mention about concepts, which influence of this perception of the Death, for example a freedom, an anxiety, a fear or a quilt. These concepts are importance to accept of the Death as a part of a human life. We can't omit the existence of God, who authors, without Karl Jaspers, decline. Diploma thesis deals with importance of the Death and what the Death tells for human. Authors concentrate on moment of the Death and subjective of the Death not only, but meaning of the Death in future too.
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