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Stability of vesicular systems using fluorescence spectroscopy techniques
Máčala, Jakub ; Venerová, Tereza (referee) ; Mravec, Filip (advisor)
This thesis is focused on possibility of studying stability and fusion of catanionic vesicles with Förster resonance energy transfer. The mainly used technique was Time-Correlated single photon counting. Firstly, excitation and emission spectra of chosen probes were measured and donor-acceptor pairs were suggested: 5-hexadecanoylaminofluorescein with Octadecyl Rhodamine B, Bodipy 493/503 with rhodamine or DiI, perylene with fluorescein, DiO with DiI. Then, time-resolved measurements of suggested pairs from environment of catanionic vesicles with different content of cholesterol were made in order to track the FRET associated with fusion of vesicles. It was found out, that it is not possible to use DiO as a donor because of it’s inefficient solubilisation into vesicles. It is also not possible for Bodipy to be used as a donor, because of it‘s excimer formation. In case of using fluorescein as a donor, it was found, that there is ongoing homo-fret between fluorescein molecules. Thanks to this, fusion was tracked by addition of unstained vesicles. It was also possible to track fusion in longer period of time. Also perylene-fluorescein pair was found to be capable of tracking the fusion, but with the exception of vesicles with content of cholesterol of 43 mol. %, tracking of fusion was possible only in short period of time.
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Tax Implications of Company Transformations
Roubíčková, Kristýna ; Ondráková, Zdeňka (referee) ; Kopřiva, Jan (advisor)
The diploma thesis deals with the business combinations. The theoretical part is focused on individual types of business combinations and their legal, accounting and tax aspects. The analytical part contains practical examples of selected types of business combinations and their subsequent comparison. In the suggestion part, these specific business combinations are evaluated from a legal, accounting and especially tax point of view.
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Binocular vision
Němcová, Andrea ; Šanda, Jaroslav (referee) ; Fedra, Petr (advisor)
This bachelor thesis deals with the physiology of binocular vision for obtaining three-dimensional perception from two-dimensional images when using special glasses. It focuses on anatomy and physiology of the human eye and vision as inseparable parts of binocular vision, and on binocular vision as such. The work also mentions terms related to vision and describes the evolution of binocular vision during the human life. The important part of this thesis is the description of the emergence of three-dimensional perception from two two-dimensional pictures (stereogram). The thesis explains the principles of 3D projection methods, especially those which use active or passive glasses, and briefly describes the possibilities of 3D projection without glasses. The practical part includes a plan of capturing dynamic scenes where important parameters which affect the video capturing are described. The plan provides a description of a dynamic scene design with respect to the possibility of verification of human physiological parameters. Described scenes were captured by two identical cameras and edited in appropriate software. These videos were shown to a group of viewers whose task was to evaluate the videos from both subjective and objective points of view.
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Assessing the Synergistic Effects of the Selected Consolidated Entity
Plačková, Michaela ; Kubovic, Pavel (referee) ; Rajchlová, Jaroslava (advisor)
This diploma thesis is focused on an evaluation of existence of synergic effects within consolidating subjects. Consolidated groups are defined by theoretical knowledge. Suitable indicators for evaluation of achieved synergic effects are suggested in the next step. Synergic effects are being observed by comparison of parent undertaking and consolidated group in chosen sphere. Based on an analysis, results are evaluated if the synergic effects are achieved.
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Assessing the Synergistic Effects of the Selected Consolidated Entity
Schreiber, Adam ; Kubovic, Pavel (referee) ; Rajchlová, Jaroslava (advisor)
This diploma thesis is aimed at the issue of synergistic effects selected consolidated entities in the Czech Republic. The objective of this thesis is the evaluation of whether the synergic effects arise in selected concerns from "Wholesale trade, retail trade; repairs and motor retail trade" industry. The decision about the presence of synergistic effects is based on the comparison of values of selected indicators for parent company and the concern.
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Tax Implications of Company Conversions
Ligasová, Michaela ; Zeráková, Petra (referee) ; Kopřiva, Jan (advisor)
This diploma thesis is focused on the transformation of business companies. The theoretical part deals with individual types of transformations, and especially their accounting and tax implications. The practical part of the thesis deals with the most common cases of company transformations, namely mergers and divisions. The last part of the diploma thesis contains practical examples and their evaluation.
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Binocular vision and anaglyph production
Klíma, Jindřich ; Jehlička, Karel (referee) ; Fedra, Petr (advisor)
The aim of my semester work was to study the physiology of binocular vision for spatial perception of two-dimensional images using glasses with coloured filters, anaglyphs. Since the theme concerns the visual sense, I explained the principle of the vision first. Then I described the principle of physiological development of the vision and the assumptions about binocular vision. The binocular vision is a complex process ongoing in the brain. Therefore, another topic of my thesis was an analysis of three-dimensional objects, space analysis, which unknowingly takes place in our brain. The next chapter deals with stereoscopy, technology which evokes a feeling of depth in two-dimensional images. One of the techniques is obtaining spatial perception by means of glasses with colour filters. In my thesis, I summarized the knowledge about the principle of anaglyphs and I described the principle of obtaining anaglyphs, from shooting the scene, over its creation to its viewing. Finally, here is an example of the program created in Matlab. It is a simple program to obtain anaglyphs from two separate frames.
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Emotion Detection from Speech
Popková, Anna ; Fér, Radek (referee) ; Matějka, Pavel (advisor)
This Bachelor Thesis deals with research in the field of emotion recognition mainly from speech and marginally from other modalities (video and physiological data). It closely describes the topology of the systems built specifically for the subject of this work. Moreover, it describes experiments leading to optimized pre-processing, regressor training and post-processing. Data used for these research origins from evaluation AV+EC 2015. Results of fusion systems producing the most precise prediction were sent to this evaluation. The Bottle-Neck features are newly tested and combined favorably with commonly used eGeMAPS features for the recognition of arousal. For valence, two kinds of video features are used. Muli-task system (recognizing both valence and arousal) using Bottle-Neck features produces competitive results and is only 13 % relatively behind the mentioned fusion system. This is especially appealing for applications where only audio is available.
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