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Modelling, parameter estimation, optimisation and control of transport and reaction processes in bioreactors.
ŠTUMBAUER, Václav
With the significant potential of microalgae as a major biofuel source of the future, a considerable scientific attention is attracted towards the field of biotechnology and bioprocess engineering. Nevertheless the current photobioreactor (PBR) design methods are still too empirical. With this work I would like to promote the idea of designing a production system, such as a PBR, completely \emph{in silico}, thus allowing for the in silico optimization and optimal control determination. The thesis deals with the PBR modeling and simulation. It addresses two crucial issues in the current state-of-the-art PBR modeling. The first issue relevant to the deficiency of the currently available models - the incorrect or insufficient treatment of either the transport process modeling, the reaction modeling or the coupling between these two models. A correct treatment of both the transport and the reaction phenomena is proposed in the thesis - in the form of a unified modeling framework consisting of three interconnected parts - (i) the state system, (ii) the fluid-dynamic model and (iii) optimal control determination. The proposed model structure allows prediction of the PBR performance with respect to the modelled PBR size, geometry, operating conditions or a particular microalgae strain. The proposed unified modeling approach is applied to the case of the Couette-Taylor photobioreactor (CTBR) where it is used for the optimal control solution. The PBR represents a complex multiscale problem and especially in the case of the production scale systems, the associated computational costs are paramount. This is the second crucial issue addressed in the thesis. With respect to the computational complexity, the fluid dynamics simulation is the most costly part of the PBR simulation. To model the fluid flow with the classical CFD (Computational Fluid Dynamics) methods inside a production scale PBR leads to an enormous grid size. This usually requires a parallel implementation of the solver but in the parallelization of the classical methods lies another relevant issue - that of the amount of data the individual nodes must interchange with each other. The thesis addresses the performance relevant issues by proposing and evaluation alternative approaches to the fluid flow simulation. These approaches are more suitable to the parallel implementation than the classical methods because of their rather local character in comparison to the classical methods - namely the Lattice Boltzmann Method (LBM) for fluid flow, which is the primary focus of the thesis in this regard and alternatively also the discrete random walk based method (DRW). As the outcome of the thesis I have developed and validated a new Lagrangian general modeling approach to the transport and reaction processes in PBR - a framework based on the Lattice Boltzmann method (LBM) and the model of the Photosynthetic Factory (PSF) that models correctly the transport and reaction processes and their coupling. Further I have implemented a software prototype based on the proposed modeling approach and validated this prototype on the case of the Coutte-Taylor PBR. I have also demonstrated that the modeling approach has a significant potential from the computational costs point of view by implementing and validating the software prototype on the parallel architecture of CUDA (Compute Unified Device Architecture). The current parallel implementation is approximately 20 times faster than the unparallized one and decreases thus significantly the iteration cycle of the PBR design process.
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The anarchistic ideas according to FSA/FAS
Štec, Ondřej ; Charvát, Jan (advisor) ; Jeřábek, Martin (referee)
Bachelor thesis "The anarchist ideals according to FSA/FAS" talks about diferant approaches to anarchism in czech context. It summerises the origin of the anarchist idea and attend to the evolution of anarchist groups in czech lands from its constitution in the end of 19. century. The most important part of this thesis is dissertating about anarchist group "Federace sociálních anarchistů/ Federace anarchistických skupin" about its behaviour and view of society. This thesis defines what was the specificity of FSA/FAS in comparison with other czech anarchist groups. It marks the ideal's root which conduced to the foundation of FSA/FAS and publication activity of that. The end of thesis estimates being of FSA/FAS and its legacy.
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Scream and Silence
Štefaňák, Ondřej ; NEBESKÝ, Jan (advisor) ; KORČÁK, Jakub (referee)
The work aims to examine the artistic expressionist tendency, manifested in expressionist drama and theater practice. In the first part, the author focuses on the origin and the direction of its general manifestations in arts and the historical context of expressionism. The author also attempts to highlight the features of expressionism in drama and contemplates the methods of expressionist artists in theater. In the second part, the author reflects his own experience of the production of an early expressionist text ''Spring’s Awakening''. He analyzes the text's dramaturgical potential and creates the basis for the theatrical production. He describes the creation of the staging as an audio-visual and scenic solution, where the particular focus is on the stylization of actors. Finally, the author tries to capture the essence of expressionism in a broader sense, as a peculiar way of expression for the artist.
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The journey of polka to the opera The Bartered Bride
Adams, Tereza ; FRIČOVÁ, Marie (advisor) ; GREMLICOVÁ, Dorota (referee)
This diploma thesis contains the description of the polka dance, its origin and short history. The next part belongs to the opera of Bedřich Smetana „The Bartered Bride“ from its creation through to the next presentations in the National theatre in Prague until the actual performance. In the last part there are descriptions of three chosen choreographies of polka from this opera and their analysis according to the model of Janet Adshead – Lansdale. Supplements contain biographies of Bedřich Smetana and choreographers of chosen inscenations – Otto Šanda, Pavel Šmok and Ladislava Košíková.
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Folk culture in memories of people born in 20s and 30s of 20th century
Horová, Kateřina ; STAVĚLOVÁ, Daniela (advisor) ; FRIČOVÁ, Marie (referee)
This thesis is focused on memories of people born in 20s and 30s of 20th century about traditional folk culture. It is focused on observing original traditions and celebrating traditional festivities and what sense had these traditions for them and how they ment it. First part of this thesis is focused on narratives life stories. Second part is about memories of traditional customs and habits and how was changing their way of celebrating during prewar period, war and in time of socialism. Last part is dedicated to dancing. Storytellers spoke about dancing opportunities, their own dance experience and dance perofrmances in the theatre.
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Injuries caused by an animal in the pre-hospital emergency care and subsequent outpatient care
VOTRUBOVÁ, Kateřina
The topic of this bachelor thesis is the various types of injuries from animals in prehosptial emergency care and their subsequent treatment in outpatient clinics. The theoretical section includes the summary of possible predicaments, which various animals cause. It discusses anaphylactic reaction to insect stings and furthermore the poisoning by snake venom, which in the Czech Republic primarily concerns the common viper (otherwise known as the common adder). The next chapter deals with the illnesses caused by cats, specifically cat scratch disease (lymphadenopathy) and toxoplasmosis with acute childbirth. The acute condition caused by dog bites and rabies belong to the next section of the thesis. In the conclusion a brief summary of injuries is mentioned, which are a threat during the handling of domestic animals, where the thesis focuses on the polytraumatized patient, fractures and cranio-cerebral injuries. The practical section was carried out on two qualitative levels in the Central Bohemian Region in connection with the Region of the Capital City, Prague and the Southern Bohemian Region. The first of these was the verification of knowledge of paramedics about injuries caused by animals through a knowledge test, comprising of ten questions. These questionnaires were always filled in my presence. In total 40 knowledge tests were distributed, of which 38 were returned completed. The return rate was thus 95%. The second section determined the differences in outpatient care in the individual regions. It was carried out through managed interviews with outpatient medical doctors from the surgical and internal medicine departments, a traumatologist, general practitioner and paediatrician. In total, eight medical doctors were approached. Furthermore an identical questionnaire was given to all these medical doctors. The research was carried out from November 2013 to April 2014. The aim of this thesis is to ascertain the knowledge about these injuries of paramedics in the Central Bohemian Region in connection with Region of the Capital City, Prague and the Southern Bohemian Region and compare them. Furthermore, it intends to find the difference in outpatient care of patients in the same territorial units injured in this way. Three research questions were asked, specifically to the mid-level health services personnel, whether paramedics have the correct knowledge about this issue and whether their knowledge varied in the regions. Regarding the second aim, the question asked was whether outpatient care of injured patients varied in the relevant regions.
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The occurrence of domestic and wild forms of the camels in the old world and their economic use
Burgerová, Michaela ; Gardiánová, Ivana (advisor) ; Jan, Jan (referee)
Genus camel (Camelus) includes two species, the Dromedary camel (Camelus dromedarius) and Bactrian camel (Camelus bactrianus). Camels have a wide range of utilization. People in Arabia countries but also in India and Egypt are using camels as a milk and meat animal, or even as a transport and companion animals and riding animals too. The aim of this thesis was to find sources about camel keeping and using and his role of farm animal in different cultures and countries. And about conditions and requirements in captivity. The first part of thesis contains information about phylogeny and origin of the camel family, taxonomy of camels, short information about the history of domestication and using of camels. There are also described the two species in terms of species biology etc. plus a brief mention of camel hybrids and camel breeds. The second part focuses on the use of camels. The preffered and most common use is in the production of milk, other dairy products from camel milk, fermentation of milk. Another use is for meat performance, meat composition, describes products. His utilization i also useful for other parts such as hair wool and mane, they come mainly from bactrian camel (Camelus bactrianus). The last is working utilization of camels, carrying baggage. Then hobby use. The thesis describes the conditions of keeping camels in captivity, keeping camels in Somalia, requirements for the supply of water and food, housing conditions in the Czech Republic, it is particularly widespread keeping camels in zoos. In Czech Republic they are small private camel breeders. For comparison they are conditions for keeping camels in captivity in Australia.
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Microbiota of the digestive tract of bees and related insect and the influencing facftors
Hroncová, Zuzana ; Havlík, Jaroslav (advisor) ; Kalous, Lukáš (referee)
This thesis consists of six research chapters, out of which, five have already been published in research journals, 1 is in the process of submission and the last chapter presents original unpublished confidential data in the process of manuscript preparation.
The research presented in this thesis concerns the complex mechanisms of bees and wasps immune system focused on microbiota as component of immunity. As shown in the introduction, highly social and managed species of bees like honey bees and bumble bees, play key roles in natural and agricultural ecosystems worldwide. Recent losses of bees have been attributed to pesticide exposure, poor nutrition, increased parasite loads and habitat degradation. Over the past several years, governments, beekeepers, and the general public worldwide have become concerned by increased losses of honey bee colonies, calling for more research on how to keep colonies healthy. Our main aim was to explore the complex mechanisms of bees and related species gut bacterial populations, their links to insect immunity and investigate the opportunities for an intervention. Part of our aim was to characterise the bumble bee and wasp gut microbiota using 16S RNA sequencing in a search for potentially novel bacterial species.
We have tried to explain how microbiome interacts with the host and showed that major members of these communities appear to benefit the host. The simple gut communities of social bees present ideal model systems to investigate the underlying evolutionary and genetic processes of such interactions. Information based on our results may help in the design of proper probiotic supplementation strategies with respect to physiological conditions in the honey bee gut.
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