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Comparison of cytokine production by leukocytes from newborns of healthy and allergic mothers
Dusilová, Adéla ; Hrdý, Jiří (advisor) ; Janatková, Ivana (referee)
The increasing incidence of children suffering from allergic diseases could be caused by sensitization of immature immune system during the intrauterine development. Several important scientific papers have demonstrated the ability of cord blood cells to respond by elevated proliferation activity after stimulation by common allergens. Following these findings, present study follows the production of cytokines which play a role in the pro- and anti-allergenic tuning of the immune system. Umbilical cord blood cells were stimulated with polyclonal activators (phytohaemagglutinin) and common allergens (ovalbumin, timothy grass, birch, mite). Subsequently, cytokine production was monitored using selected methods that reflect different stages of cell activation - at the level of mRNA by quantitative real time PCR (qRT-PCR), by flow cytometry detection of the presence of intracellular cytokines in different cell subpopulations and by ELISA measurement of cytokines in CBMC culture supernatants. The results obtained point to a very weak ability of these common allergens (timothy grass, birch, mite, ovalbumin) to stimulate CBMC to produce cytokines observed by all of these methodological procedures. Although we did not observe significant differences in CBMC cytokine production (IL-2, IL-4, IL-10, IL-12,...
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Cultural support in Prague's districts
Mokrá, Jolana ; KAŠPAR, David (advisor) ; PROKOP, Petr (referee)
Diploma thesis ”Cultural support in Prague's districts” focuses on a current situation in municipal strategical management and support of culture in all Prague districts. The introduction emphasises the importance of each level of government in the management and support of culture through comparing specific cultural policies. The research is divided into two parts. First one depicts how culture is being understood on the municipal level and presents an overview of existing instruments for support and planning. The second part is focused on a few municipal districts that handle their culture policy with a particular strategy and consider it to be an irreplaceable part of a local development. The second part also examines what tactics and tools these municipal districts use for a realization of their strategic goals. In conclusion, this thesis proves why the role of the local official government is so important in the developement and implementation of culture.
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Role of Psb28 proteins in the biogenesis of the Photosystem II complex in the cyanobacterium Synechocystis sp. PCC 6803
BEČKOVÁ, Martina
The thesis focuses on the role of Psb28 proteins, namely the Psb28-1 and its homolog Psb28-2, in the biogenesis of the Photosystem II complex (PSII) in the cyanobacterium Synechocystis PCC 6803. The aims of this work were to localize the proteins within the cells, and to determine their function. A fraction of both Psb28 proteins was identified in the monomeric PSII core complexes but most proteins were found in the unassembled protein fraction associated with thylakoid membranes. Psb28-1 was mostly detected as a dimer while Psb28-2 as a monomer. Psb28-1 also differed from Psb28-2 by its higher affinity to the PSII core complex lacking CP43 antenna. Characterization of Psb28-less mutants suggested regulatory function of the proteins in PSII biogenesis in connection with chlorophyll biosynthetic pathway. Analysis of preparations isolated using FLAG-tagged versions of Psb28 proteins showed their association with Photosystem II - Photosystem I supercomplexes, especially under increased irradiance, and supported a role of Photosystem I in the PSII biogenesis.
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The Media Influence on Transformation of Democracy
Půlpánová, Barbora ; Pehe, Jiří (advisor) ; Slačálek, Ondřej (referee)
Mass media has always played the key role in democratic process. They are not only the indicator of its right functioning, but also the iniciator. From historical point of view is obvious, that technological progress and changes in the way of informing people were related to the political area. But with the increasing role of media, they changed their position from pure mediators of informations to the main actors of social and political life. The ‚old' media, such as newspapers, radio and television, are therefore partly responsible for commercialization of the public sphere and also for its depolitization. With contribution of the globalization effect on today's world, which causes besides other things the weakening of the nation state, there are only a little of the key democratic aspects left. Some find the solution in the ‚new' media represented by the Internet and social media, which are more interactive so they could get the citizens back to the game. And right in the increase of civic engagement see the theorists the potencial for creating a new democratic order based on high-level participation. KEY WORDS Mass media, Democracy, Public Sphere, Civic Engagement, Internet, Social media, Participative Democracy, Deliberative Democracy
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Evaluation of the effectiveness of teaching vocational subjects depending on physical activity before schooling.
Janošíková, Martina ; Hanušová, Marie (advisor)
The period of transition to secondary school brings substantial changes in the requirements for study obligations, autonomy, communication, expression, or separated from their families in the form of staying at the dormitory. Students enrolled in sport centers, in addition to these changes, still struggling with the responsibilities associated with sport. Because they are students first through fourth grades of secondary schools and secondary vocational schools, an age when image self-I am, what I believe in, arises in interactions with the outside world and their own experience, plays a very important role to influence the environment, educators, teachers and trainers, with which students spend most of their time in practice. This study looks at students comprehensively in terms of personal development as a multifactorial biopsychosocial unit, in terms of cognitive and motivational processes, states and properties from the perspective of social psychology dealing with the interactions, relationships and communication, psychology of individuals within the social group and its importance for the development of the individual . It monitors conditions and educational outcomes in terms of educational psychology, dealing with forming properties, opinions, attitudes and value orientation from the perspective of educational psychology.
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Fish passes and their role in landscape and ecosystem management
Míka, Martin ; Horký, Pavel (advisor) ; Kuříková, Pavlína (referee)
Fishways are devices intended to restore fish migration through the transverse obstacles in fragmented flows. Basic types of fish passages can be divided into technical and close to nature. Technical as cellular or crevice between the close to nature can include, for example, a bypass channel or boulder ramps. Close to nature fishways are suitable not only for Migration wider range of species and the size of fish, but they are preferable as landscape elements in urban landscapes. Can be recommended to be close to nature when crossing streams fragmented preferred, although not necessarily their use, for example, due to space constraints at a particular location possible.
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Microbiota of the digestive tract of bees and related insect and the influencing facftors
Hroncová, Zuzana ; Havlík, Jaroslav (advisor) ; Kalous, Lukáš (referee)
This thesis consists of six research chapters, out of which, five have already been published in research journals, 1 is in the process of submission and the last chapter presents original unpublished confidential data in the process of manuscript preparation.
The research presented in this thesis concerns the complex mechanisms of bees and wasps immune system focused on microbiota as component of immunity. As shown in the introduction, highly social and managed species of bees like honey bees and bumble bees, play key roles in natural and agricultural ecosystems worldwide. Recent losses of bees have been attributed to pesticide exposure, poor nutrition, increased parasite loads and habitat degradation. Over the past several years, governments, beekeepers, and the general public worldwide have become concerned by increased losses of honey bee colonies, calling for more research on how to keep colonies healthy. Our main aim was to explore the complex mechanisms of bees and related species gut bacterial populations, their links to insect immunity and investigate the opportunities for an intervention. Part of our aim was to characterise the bumble bee and wasp gut microbiota using 16S RNA sequencing in a search for potentially novel bacterial species.
We have tried to explain how microbiome interacts with the host and showed that major members of these communities appear to benefit the host. The simple gut communities of social bees present ideal model systems to investigate the underlying evolutionary and genetic processes of such interactions. Information based on our results may help in the design of proper probiotic supplementation strategies with respect to physiological conditions in the honey bee gut.
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The role of credit rating agencies in 2008/2009 global financial crisis
Andrlíková, Petra ; Půlpán, Karel (referee) ; Teplý, Petr (advisor)
This bachelor thesis aims at the role of rating agencies in the pending economic turmoil. It explains main functions of credit rating agencies (CRAs) with the relation to the 2008/2009 global financial crisis. Furthermore, it describes the proposals and solutions to the current situation on financial markets adopted by regulatory institution in the European Union and the United States, as well as the responses of CRAs on the proposed and adopted regulation. In particular, the work suggests the level of coverage of current amendments in order to prevent such failure in the future. Moreover, the thesis discusses possible solutions and improvements of the CRA business model.
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The role of authority and form of partnership during creation of new choreographic dance piece
Radačovský, Mário ; KAZÁROVÁ, Helena (advisor) ; WIESNER, Daniel (referee)
In our dance life its very important that we meet time to time people who are so incredibly influencetial to us ether positive or negative ways. Some of them are able to influence us so much that we keep this impact which they have made on us for ever.Role of autority and partnership between these two elements is crucial.Its has impact on everyday of our working life.I ca not imagine what can happen if there is not existing somekind of form of partnership in the dance studio.To be dancer is very no simply just job .Is more ,its something truly special what not everyone can understand.The task of my work is to go back in my profesional past ,through out my own memories and profesional experiences to offer some situations when I truly felt presents of authority and partnership .Sometimes only autorithy .I this work Im trying to offer my small stories which I lived thourgh from my prospective and Im tring to analysed them through my own expierience of today.It always about something.We are creating because we want to say things.Journey is very important sometimes more then final outcome.
Now important during this journey is able to see how things are done how we comunicate them and autorities will appear .
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EU's Role in the Middle East Peace Process and Comparison with Other Significant Participants
Špiler, Jan ; Dubský, Zbyněk (advisor) ; Havlová, Radka (referee)
The topic of this thesis is the question of EU's involvement in the resolution of the Middle East conflict (in a narrower sense the conflict between Israel and the Palestinians) and the survey of common positions and differences of the EU and other significant participants (especially the USA and the USSR/Russia). The ultimate objective is to assess the contribution and role of the EU in the whole process based on its analysis. The first part of the thesis outlines the evolution of the Middle East conflict from the creation of the State of Israel up to the present (May 2008), the second part deals with the analysis of West European states' and EU's approach towards the Peace Process and the the third part contains a comparison of the EU's positions with selected key participants (the USA and the USSR/Russia).
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